BACKGROUND:
I want to use the red, green and blue
(8nm-wide) Baader Astronomy filters for the
image-stacking technique from the "Microscopy Today"
magazine. This is for microscopy on
human circulatory and blood components
at magnifications of 400X to 1200X.

One of the advanced techniques,
which will only be considered after
my question below is answered is that
if the specimen is mostly red,
the "regular, non-filtered-white-light-illuminated"
image can be the "red-component" in the "RGB" image-stack.

THE PROBLEM;

FOR THE GREEN FILTER,
DO I USE A 500nm FILTER OR A 540nm FILTER??

There's conflicting information on using
the 500nm filter or the 540nm filter for the
green color (both are about 8nm-wide) .

ITEM 1:
In the January 2009 article by Dr. Jörg Piper,
he uses a 540nm filter for green.

ITEM 2:
In the September 2008 article by Dr. Jörg Piper,
he mentions a 500nm green filter in the paragraph labeled:

"Monochromatic green filters"...

"…when a Solar continuum filter (540 nm) is used;
it is STRONGLY CONTRASTED BY THE O III FILTER
(500 nm). IN THIS RESPECT, THE O III FILTER
MAY BE REGARDED AS A SUPERIOR GREEN FILTER,
combining the contrast effects of the solar
continuum filter and the H beta filter
(see table 1)…" – H beta is a 486nm filter.

Thank you so much!

Carl