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Anybody try 'Diffracted Light Contrast -- DLC' as explained in Micr   Message List  
Reply | Forward Message #33819 of 53052 |
Has anyone tried this?

Microscopy Today, Nov. 2006, has an article on DLC -- Diffracted
Light Contrast. It seems its a simple 'oblique' technique, using a
knige-edge at the filter position of the condenser to cast a
diffraction pattern on the specimen. It refers to this abstract:

Diffracted-light contrast enhancement: a re-examination of oblique
illumination.Piekos WB.
Department of Molecular, Cellular and Developmental Biology, Yale
University, New Haven, Connecticut 06520-8103, USA.
barry.piekos@...

A re-examination and modification of the very old oblique
illumination technique has resulted in a method for contrast
enhancement in microscopes, diffracted-light contrast (DLC), which
provides high-contrast, high-resolution images of unstained
biological material. The technique, which utilizes the diffracted
light from the edge of a small, opaque plate, provides shadowcast
images similar to those obtained by Nomarski DIC, anaxial
illumination, modulation contrast, or single-sideband microscopy;
however, it requires only a single additional component, which can be
added to any bright field microscope. The contrast and three-
dimensionality of the final image can be controlled by inserting
differently shaped edges. Any bright field condenser will work with
the technique and, consequently, it is a technique that may be
especially useful with relatively basic, inexpensive laboratory and
teaching microscopes although the image





Wed Dec 6, 2006 1:56 am

psneeley2003
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Message #33819 of 53052 |
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Has anyone tried this? Microscopy Today, Nov. 2006, has an article on DLC -- Diffracted Light Contrast. It seems its a simple 'oblique' technique, using a ...
Steve Neeley
psneeley2003
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Dec 6, 2006
2:08 am

See "poor man's dic" in this article: http://www.microscopy-uk.org.uk/mag/artdec03/wdonion2.html...
gc
gc426826@...
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Dec 6, 2006
4:19 am

Yes, I wondered if Pseudo-Phase was similar, but this DLC uses a diffraction edge with a de-focused condenser (similar to Pseudo-phase but there I think you...
Steve Neeley
psneeley2003
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Dec 6, 2006
5:44 am

I put a picture in the Don Williams Photo Gallery of some of the plastic inserts I've used. They are arranged so that the points are on the same plane as the...
Don Williams
edfwilli
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Dec 6, 2006
6:55 am

The patent, the paper in Microscopy Today, and the abstract, all refer to placing the edge at the field stop -- which I interpret to mean the field diaphragm....
Steve Neeley
psneeley2003
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Dec 6, 2006
6:30 pm

At lest we can't complain about the lack of images, instructions or openness of the method used in US patent #6600598 ...
Gordon Couger
gcouger
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Dec 7, 2006
8:45 am

... I have used some type of compound microscope since I was 8 - a LONG time ago. I can remember those inexpensive, at first plastics, then Edmond monoculars...
interlofer
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Dec 7, 2006
4:50 pm

It's not the improved contrast but the increase in resolution claimed in the first and second image that I find hard to explain by the simple methods he used....
Gordon Couger
gcouger
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Dec 7, 2006
6:27 pm

"....Since he had to borrow a resolution slide I don't expect he was working on increasing resolution." .... Heh! That's kinda funny. Eureka! I'll try to wade...
interlofer
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Dec 8, 2006
4:54 am

Haven't got access to Microscopy Today unfortunately, do they show images with superresolution? What exactly do they say about it? I can only assume they can...
rvanwezel
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Dec 8, 2006
12:03 pm

I briefly looked at the patent and the article, and,....Isn't this technique very similar to the animated - java tutorial (below) from the Nikon web site? Is...
rich_guy_looker
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Dec 11, 2006
1:20 am

I would bet this was well understood 100 years ago...
gc
gc426826@...
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Dec 6, 2006
7:02 pm

By putting the edge at the field stop it would be in focus int the same plane as the subject. It's not hard to understand with the illustration at...
Gordon Couger
gcouger
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Dec 6, 2006
8:55 pm

Does this "DLC" method use only one knife-edge, or two? Mention of knife-edges makes me think of the Schlieren method to image variations in the refractive...
Kevin
k9ev
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Dec 7, 2006
10:08 am

Isn't this somewhat the same as the technique where you mount a piece of copper wire diagonally in the objective?? Saw it years ago on the web, but no idea...
rvanwezel
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Dec 7, 2006
10:39 am

The wire has to be at the back focal plane of the objective. This means you have to make a saddle shaped wire that goes down into the objective and is cemented...
Don Williams
edfwilli
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Dec 7, 2006
11:56 am

... This is nonsense. The wire has to be anywhere but the back focal plane of the objective -- but where exactly I didn't know. I'll look for the original...
Don Williams
edfwilli
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Dec 7, 2006
2:46 pm

Hi all, For those interested in the schlieren imaging aspects of this technique, there is a fascinating article in "American Scientist" V94 N 1 Jan-Feb 2006...
Matt.Kitching@...
mattkitching
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Dec 11, 2006
2:11 am

Hi all, A colleague of mine, who works for a museum and has little extra funding, was about to buy some scanned docs on ebay when I pointed him to these sites:...
DJH
djhmis
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Dec 11, 2006
2:35 am

Correction: I say the back focal plane of the objective. This is nonsense. But I don't know where the wire should actually be -- I'll try to find the original...
Don Williams
edfwilli
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Dec 7, 2006
3:55 pm

Was it this one? I'm thinking of trying it... http://www.schwaben.de/home/mathias/emikro/kontrast2.html Regards, Daron ... [Non-text portions of this message...
Daron Edie
darby9992002
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Dec 7, 2006
3:16 pm

This is the method, but not the article I read. It still doesn't tell us where the wire is supposed to be -- 5mm behind the uppermost element is rather vague...
Don Williams
edfwilli
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Dec 7, 2006
4:40 pm

No sorry, never tried it, just remembered it. It seems like another 'grayfield' technique where most of the direct light (which is heavily aperture limited) is...
rvanwezel
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Dec 8, 2006
11:57 am

Daron, I am not supporting DLC there is no room in the conventional physics of light for that resolution. I am playing with it and it is an oblique method. I...
Gordon Couger
gcouger
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Dec 11, 2006
7:48 am

Hi Folks, I have obtained permission from Barry Piekos, the author of the patent and article to post this link to a pdf of the Microscopy Today article so...
Tim Wilson
ryzhomer
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Dec 11, 2006
6:56 pm

Hi all, Here is a condenser that is similar to ones I've seen before, but I can't get a definite answer on its purpose or the models for which it is designed. ...
DJH
djhmis
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Dec 11, 2006
10:40 pm

... Tim, I am only an amateur microscopist, so I won't bore you with my failures to reproduce the results. However, from a theoretical standpoint... I have...
Rik Littlefield
rjlittlefield
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Dec 13, 2006
8:17 am

Hi Rik, Thank you for your report. I'm assuming you saw no enhancement of images whatsoever. I'll pass your suggestion on to Dr. Piekos. Salutations, Tim ... ...
Tim Wilson
ryzhomer
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Dec 13, 2006
3:38 pm

Apparently, the "long-standing theory" has been shaken up, but via another method. I ran across this article regarding a new method of fluorescence 3-D light...
duanefrybarger
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Feb 7, 2007
9:19 pm
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