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Seeking dichromatic mirrors for Zeiss III RS epi-fluorescence conden   Message List  
Reply | Forward Message #50210 of 53653 |
RE: [Microscope] Re: Seeking dichromatic mirrors for Zeiss III RS epi-fluorescence condenser

Hello David,

The first surface mirrors are not easily to obtain. I have
needed them myself. The best source for them I know of is
to use the one in a Polaroid SX-70 camera. They are dirt
cheep on Ebay and optically near perfect. As for cutting
them to a circle. That is some skill. When I did glass
work ; mark the circle with a china marker with a template
on the mirror surface and do as many straight cuts as you
can then use a glass router table(stain glass hobbyist use
them for any inside glass curves needed) it is with a small
diamond wheel and gently follow the china marker line.
Someone in the group might know a better way to do a glass
circle.



Hope this helps.



Kind Regards,



Charla



_____

From: Microscope@yahoogroups.com
[mailto:Microscope@yahoogroups.com] On Behalf Of pennine56
Sent: March 13, 2009 06:36
To: Microscope@yahoogroups.com
Subject: [Microscope] Re: Seeking dichromatic mirrors for
Zeiss III RS epi-fluorescence condenser



Hello Fred and René

Many thanks for your suggestions, I'll contact them.

The Zeiss III RS head predates the filter cube design, which
in a way is good because the excitation / barrier filters
and mirrors all readily come out of different mounts.

It is the circular mirrors with first surface coatings that
have failed. Although the filters were cooked to a rough
semi-opaque finish these polished up a treat at home with
some elbow grease. Not sure what the fine abrasive is in
silver polish (alumina?) but removed this rough finish on
all the filters to give back a high polish and transmittance
without scratching the glass.

regards
David
>





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Fri Mar 13, 2009 11:27 pm

nordicwomanca
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Message #50210 of 53653 |
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Was wondering if anyone knew of a source of the dichromatic mirrors that were fitted to the Zeiss III RS epi fluorescence condenser. I checked with Zeiss UK to...
pennine56
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Mar 12, 2009
12:59 pm

Does ayone know how long MRSA can survive in a typical household evnironment devoid of people? Is much longer at temperaturess of say 40 F? Thanks, John...
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j_forster911
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Mar 12, 2009
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   Hi John, I just e-mailed you a Medscape article...covers a lot.  Hours to months the residence time for MRSA on surfaces.  But this NOT the issue so...
Charles Guevara
icecilliate123
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Mar 12, 2009
3:32 pm

Hi, Thank you. My concern is the risk of getting a MRSA infection if a person has an open wound, on enterng and living in a space tht has not been occupied in...
J. Forster
j_forster911
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Mar 12, 2009
5:01 pm

Good summary Charlie! I have been off work on illness leave for most of one year, because of a MRSA infection and it's after-effects on my left leg. In fact, I...
Steven Born
stevenbornsfv
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Mar 12, 2009
5:24 pm

The thing most overlooked is that the best defense against a harmful staff is the normal staff that is present on everyone. You can use drugs and solutions...
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alfredscherer
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Mar 12, 2009
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John & Steve, One reason MRSA is hard to kill is by the time it is a problem it is part of biofilm. The biofilm helps protect from the antibiotic so the...
Gordon Couger
gcouger
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Mar 15, 2009
6:58 pm

Moderator please note this is not a microscope question. Should it be here??????...
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Mar 12, 2009
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John, Bacteria and other things that microscopes are used to look are obviously within the bounds of the group and have been many times in the few months you...
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gcouger
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Mar 12, 2009
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Gordon, Look at the threading. I did not bitch about OT posts. I asked the original question and have been in this Group for years, not months. In the same...
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Mar 12, 2009
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John This is the sort of thing that has been computer modeled successfully for years By dominant, I'm guessing you mean the organism present, which is most ...
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Mar 13, 2009
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So, do they do a population survey of what has grown out and work back to the starting population, using known data? John...
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j_forster911
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Mar 13, 2009
1:06 am

John This is one of those types of situations, which until the seventies, and increased computer availability, was not easily analyzed The traditional method...
Steven Born
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Mar 13, 2009
5:58 am

Dar John My deepest apology to you for the mistake. I don't know why I picked your name instead of Armand squatule777@....... I am composing a post off line to...
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gcouger
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Mar 13, 2009
1:49 am

Thanks, John...
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j_forster911
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Mar 13, 2009
2:02 am

   Hi Gordon..long as you are 'up in forum'..may I ask that somewhere in files, or in the 'welcome to forum content'...could Frezz, you and the other...
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icecilliate123
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Mar 13, 2009
1:25 am

   Hi Gordon, microbes in their mattrix have been the predominate life metabolizing /terra forming ( oxygen in earth atmosphere a microbial huge terraforming...
Charles Guevara
icecilliate123
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Mar 15, 2009
7:53 pm

Dear Charles, Sorry for the delay on the reply. If your intersted in biofilms I talked to one of the aurthors of this paper. The Winogradsky plate a convenient...
Gordon Couger
gcouger
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Mar 19, 2009
4:04 am

   Thank Gordon for bringing Winogradsky into this thread on MRSA.  Tennis ball plastic containers are ideal for Winogradsky columns.  You can simply foil...
Charles Guevara
icecilliate123
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Mar 20, 2009
3:07 am

Hi Charles, This reference should allow your library to get it for you via interlibrary loan. Most papers come in 24 to 25 hours now. I thought I hand moved my...
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gcouger
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Mar 20, 2009
7:57 am

{posted on Amateur_Microscopy by Glenn Shipley. Forwarded by gordon} Hi Everyone: There is an interesting thread about MRSA (Methicillin Resistant Staph...
Gordon Couger
gcouger
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Mar 20, 2009
7:59 pm

chroma.com, omega.com and semrock.com all produce dichromatic mirrors for microscopes. Usually about $125 each for older filters such as yours. I expect they...
fred.hebard
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Mar 13, 2009
12:44 pm

... Hello Fred and René Many thanks for your suggestions, I'll contact them. The Zeiss III RS head predates the filter cube design, which in a way is good...
pennine56
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Mar 13, 2009
1:36 pm

... Hi David, What you did will work with the old-style glass filters, which yours undoubtedly are. The newer filters are interference filters, so I'm just...
fred.hebard
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Mar 13, 2009
2:28 pm

I should have said "old-style, DYED glass filters" in the previous post....
fred.hebard
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Mar 13, 2009
2:30 pm

... Hi Fred A good point. Yes, they are Schott 3 mm thick dyed glass filters in my III RS although notice from the Zeiss docs that there were interference...
pennine56
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Mar 13, 2009
2:38 pm

Hello David, The first surface mirrors are not easily to obtain. I have needed them myself. The best source for them I know of is to use the one in a...
Charla Mason
nordicwomanca
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Mar 13, 2009
11:27 pm
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