Dave, ... Thanks very much Dave for this explanation. Some members may consider it to be nit-picking, but I believe it's very important that we all understand...
... Do you know what they mean by this? Do they simply discard the low 2 bits? Or apply a nonlinear coding method? Or lossless compression so that 12 bits...
The pdf document available from the link below might be useful to some group members. It looks very helpful and I shall give it a go when next I spot a diatom....
... First, let me say that I am speaking specifically of the Nikon D70/D70s. The way that I read the documentation, they are applying a nonlinear compression....
Dear Keith, Your explanation of the "smoothing" of the image, having in mind that in most cases it is used to make portraits which generally benefit from less...
... It is also true of other Nikon models. The D2x for example uses the same form of compression. However, there are some variations. The D1 (Nikon's first...
My Ortholux has a press-in metal ring that I assume is meant to hold the glass that prevents one from spilling one's drink on the mirror. Mine has no such...
Scott, All the glass from the Leitz scopes of that era are same size. Finding junker on ebay may be the best deal. If I remember right the glass is thinner...
I recently had some glass circles cut to fit a Watson stereo stand. They were cut for me by a local glass merchant here in the UK. I had a couple of clear...
Hi, all, This is really addressed to Reichert owners in the UK. I recently bought a second-hand wooden microscope box that was locked but had no key. After...
Dan, If I ever saw a case for stacking this is one. Half a dozen exposures is about all you'd need, but 12 wouldn't hurt. Don W ... -- Dr E D F Williams ...
Don, I stacked the same frames, one with Helicon and one with CombineZM and put them in my photos. I have a new camera that is supposed to be delivered today...
Scott, I'd be happy to make this piece for you since I make parts for microscopes and cameras routinely. Or, if you'd like, just contact me off line and I'll...
It appears from the illustration that I'm looking at (below) - which describes the illumination concept known as Total Internal Reflection Fluorescence...
... http://tech.ph.groups.yahoo.com/group/Microscope/photos/browse/d6bb Dan, thanks for posting out these stacked results. There are a couple of interesting...
Some weeks back, somebody posted that "spirochettes" and the organisms to detect Lyme Disease are mostly (or only?) viewable through darkfield - illumination....
Hi Rik and Erik I am not sure I understand what I am looking for. I posted two new stacks from the same photographs. I didn't crop this time and they should...
... organisms ... darkfield ... for ... (1) Even a blank slide will work. The tiniest amount of dust shows up in darkfield. How you set it up depends on your...
There are a number of ways to get TIR to work. Ely Silk has the simplest http://www.sas.org/E-Bulletin/2002-07-19/features3/body.html. But I believe it only...
In my limited experience, using dedicated DF condensers, the key issue is correct vertical alignment of the components in the optical axis. Once that is...
... Dan, I'm now looking at images named HeliconFocusStack and NewCombineZMStack, in their "Large" sizes. In HeliconFocusStack, which is 800x687 total pixels,...
you could use FastStone Image Viewer to flick the two images David ... From: Rik Littlefield To: Microscope@yahoogroups.com Sent: Wednesday, May 02, 2007 7:29...
The resolution tables I previously uploaded to the "Files" section of the group have been revised and now contain: 1. Resolution by aperture with typical...
Hi: I use Darkfield illumination to visualize structures that are smaller than the limit of resolution of the microscope. There are published accounts of how...
Confusion here. TIFF is a file format. Tagged Image File Format. JPEG is an image format. CCITT group 4 is an image format. Sloppy documentation by a lot...
I think talking about compression methods and raw format may be talking about apples and oranges. The beauty of raw out of the camera is that the raw file...
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