Hi,What I find is that when I change the film speed to a faster one say 50 asa to100 asa the file size gets bigger, but the image is not quite so good. David ...
... That's not unusual. What's happening is that higher ISO adds noise, but does not add image info. So your file gets bigger -- in order to represent the...
Thanks for your help Charlie, but I'm now having an even odder time with this program (Olympus background subtraction). I've uninstalled it and Java,...
Hi, What you really need is a simple way to achieve background correction in Photoshop or a similar image editor which allows you to use layers. There is an...
Hi Ray, What if you download Open Office from Sun Microsystems might work then. David ... From: leitzscope To: Microscope@yahoogroups.com Sent: Tuesday,...
Still looking for microtome knives? I have lots, sharp, ready to go and a lot of other Histology,EM,Microscopy and lab equipment. List available. Markus Simon...
I would like to know, what would be the diameters and focal lengths of 2 plano-convex lenses to make a high NA darkfield condenser? I don't know what the rules...
Why do you want to do this? eBay is crawling with good dark-field condensers so it's not necessary to waste your time and money trying to make one. One is...
Don: Thank you for your response, and I'm not being sarcastic. I want a darkfield made of magnesium fluoride glass, capable of passing radiation as narrow as...
I would like to know, what would be the diameters and focal lengths of 2 plano-convex lenses to make a high NA darkfield condenser? I don't know what the rules...
Ron: (1) Modifying the condenser will not work, because I need magnesium fluoride glass to pass radiation at 200-250 nm to activate the fluorescent stain dyes...
... Exactly ! I am sure if you photograph the same subject twenty or even fifty times, the later images will normally be superior to your first attempts. David...
Rich, No matter how many times I look at "Table 2", I see the top of the table stating : "Number of Reflecting Surfaces". The two systems you have stated are :...
Ron: The Cardioidal "cardoid" and "Parabolic" refer to "polished and donut-shaped circular mirrors" in the condenser. Aspheric and spheric refer to individual...
Hi Rich, Just thinking, why does the UV need to be at a low angle and not just a normal transmitted light UV setup? The barrier filter will block all the UV...
Hello Charlie, Do you know how many pixels you need for wide-field-microscopes such a Leitz Otrhoplan with a field of 28mm? Just add a factor of 1.6x ?? I use...
... The whole concept is explained here :- http://www.microscopyu.com/tutorials/java/digitalimaging/pixelcalculator/index.html It will tell you the required...
Hello David Thank you for your reply. I will have a look at pixelcalculator-link. BTW: The factor of my relay-lens is 0,32x. A relaylens 0,24x should be better...
Gordy: There are many, many specimens that emit visible radiation when deep or vaccuum wavelength radiation is applied (in addition to the use of fluorsecent...
... Hi, When you are talking about what is colloquially called "vaccuum UV," that is usually considered to be below 200nm in wavelength, down to about 180nm....
Don: Realistically speaking, I'd like to be able to use the ethanol- based fluorescein dye, which excites at 214nm, and is more suitable to deuterium vapor...
Hi again Rich, I can't really help on suggesting any optical engineers, and the questions about the Zeiss stereo microscopes are a bit out of my area so I'll...
Kevin & Gordy: I understand the concept of using an old-fashioned barrier filter above the objective in a non-epifluoresecent radiation setup, realizing that...
Hello Fred, About a year or so ago I had prepared a spreadsheet (drawing upon the link David sent to you along with other references) in order to calculate and...
... Just as a matter of interest, that would be extremely low for a projection eyepiece but a possible value for a camera-lens/eyepiece combination. For...
Hi David, Fred asked about a very wide 28mm field size to be recorded onto a DSLR sensor with a diagonal of 31.7mm. If his total relay magnification were 1.2X...
Hey Everybody, What is the best way to view opaque objects under a light microscope? Would a good flashlight that produces a 135 lumens work in viewing opaque...
Does anybody know how to preserve protists or other micro organisms that are found in water? When I am traveling, I would like to preserve water samples that...
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