Hamid Reza Shahbaazi <hshahbaazi@...> wrote:Hi (Salaam),


Analysis of Testosterone and its Metabolites by HPLC

Condition:

Column:

150 x 4.6 mm, 3 µm

Cacellpak C18 UG120 (Shiseido)

Mobile phase:

A = water

B = CH3OH, C, CH3CN

Column compartment:

40 ºC

Injection vol.:

25 µl

Detector:

diode-array detector

wavelength 245 nm

reference 450 nm


Best,


mina minaee wrote:
Hello

I intend to determine following hormones in serum with HPLC:
Testosterone, estrogen and cortisole.
I have a HPLC with PDA and flourescence detectors, 3 pumps and some columns.
Could anyone help me with mobile phase and analysis condition?

Thank you in advance and looking forward to hearing from you.

Best wishes


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