Hello (salam)

Mr Shahbaazi

Thanks a lot for your guidance about determination of testestrone. Could you
tell me if it is isocratic or gradient and how much is proportions, please?

Thanks in advance and looking forwar to hearing from you.
Minaee


Hamid Reza Shahbaazi <hshahbaazi@...> wrote:
Hi (Salaam),


Analysis of Testosterone and its Metabolites by HPLC

Condition:

Column:

150 x 4.6 mm, 3 µm

Cacellpak C18 UG120 (Shiseido)

Mobile phase:

A = water

B = CH3OH, C, CH3CN

Column compartment:

40 ºC

Injection vol.:

25 µl

Detector:

diode-array detector

wavelength 245 nm

reference 450 nm


Best,


mina minaee wrote:
Hello

I intend to determine following hormones in serum with HPLC:
Testosterone, estrogen and cortisole.
I have a HPLC with PDA and flourescence detectors, 3 pumps and some columns.
Could anyone help me with mobile phase and analysis condition?

Thank you in advance and looking forward to hearing from you.

Best wishes


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