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#1052 From: Sam Droege <sdroege@...>
Date: Thu Apr 15, 2010 3:29 pm
Subject: New video on gluing bee specimens to insect pins
sam_droege
Send Email Send Email
 

All:

Below is the link to our latest video...in this how to glue small (and large) bee specimens to pins.  

http://www.youtube.com/user/swdroege#p/a/u/0/9KfLCmYOKtA

You can also look at our other videos on various topics related to the survey of bees and the handling of the resulting specimens on our Youtube channel:

http://www.youtube.com/user/swdroege

Feel free to pass around or embed in websites.

Thanks

sam

                                               
                                             
Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

Poets and Scientists Find Boxelder Bugs
Useful for Both Metaphor and Experiment


Crush a boxelder bug.
After the little snap
a tiny liquid drop
the color of honey comes
out on your thumb.
The boxelder bug does not
hear this sound.
The red racing stripes on
his black back, like decorated
running shoes, finally don't
run anywhere, anymore.
You, on the other hand, had done
what your life prepared you for:
kill something useless and innocent,
and try to find some beauty in it.
            Bill Holm




P Bees are not optional.

#1053 From: Eric Mader <eric@...>
Date: Thu Apr 15, 2010 7:07 pm
Subject: Re: Adobe/Bank nesting bees and building nests for such
eric@...
Send Email Send Email
 

Interesting topic, Sam!

 

I was talking with Matthew Shepherd here in the office and we had one thought to add to this discussion.

 

What do folks think is the potential for these structures to become a population sink in their local landscape as they age and potentially become infested with chalkbrood spores and pollen mites?

 

I know from my days managing alfalfa leafcutter bees that parasites and diseases build up incredibly fast any time nesting substrates are repeatedly re-used by solitary bees.

 

On the one hand I think a certain level of pathogens and nest parasitism can be interesting from an educational standpoint, and can really engage people in the complexities of bee life. On the other hand, I have seen old nest blocks that are so contaminated that no new eggs deposited in them survive. Such a scenario seems likely to discourage rather than engage the general public if these types of walls are on formal display.

 

For large scale leafcutter and mason beekeeping most people have moved to loose-cell management or elaborate nest phase out systems to maintain bee health. With fixed substrates, arranged in these beautiful and artistic configurations, that seems like a challenge.

 

Anyone have thoughts on this?

 

Cheers!

 

-Eric



On Wed, Apr 14, 2010 at 10:21 AM, Sam Droege <sdroege@...> wrote:
 


All:

Today, Shelley Small passed on the following link regarding "Insect Walls."  These essentially being elaborate and artistic structures for hole nesting species.

http://greayer.com/studiog/?p=4529

Very nicely done and some neat designs for Nature Centers to contemplate.

I e-spoke to the author, Rochelle Greayer, about the fact that some species readily nest in the walls made of adobe or earthen plasters.  In my strawbale/adobe house house I have Anthophora abrupta (hundreds), Anthophora plumipes (introduced and unfortunately increasing), Ptilothrix bombiformis (small numbers), Melitoma taurea (small numbers), and numerous Osmia, Megachile, Chrysidid, micro-hymenoptera hangers on nesting.  

She would be interested in putting up a bit more about bees that live in adobe structures, earthen walls, or, better yet, structures of earth that people have made for these species.  So, if you have any examples, stories, or observations from around the world please share (you can post to me or to the list...I think it is of general enough interest that it would be good to have archived).

Thanks, as always.

sam


                                               
Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

I rose from marsh mud

I rose from marsh mud,
algae, equisetum, willows,
sweet green, noisy
birds and frogs


to see her wed in the rich
rich silence of the church,
the little white slave-girl
in her diamond fronds.


In aisle and arch
the satin secret collects.
United for life to serve
silver. Possessed.


      -Lorine Niedecker





P Bees are not optional.



--
----------------------------------------------------------------------
Eric Mader
National Pollinator Outreach Coordinator
The Xerces Society for Invertebrate Conservation
Tel: 503-232-6639 Fax: 503-233-6794
Email: eric@...
Skype: eric_mader_xerces_society

Assistant Professor of Extension
University of Minnesota - Department of Entomology
Email: made0002@...

The Xerces Society is an international nonprofit organization that protects wildlife through the conservation of invertebrates and their habitat. Our Pollinator Conservation Program works to support the sustainability and profitability of farms while protecting pollinator insects. To join the Society, make a contribution, or read about our work, please visit www.xerces.org.

Find all the information you need to conserve pollinator habitat at:
http://www.xerces.org/pollinator-resource-center/
----------------------------------------------------------------------

#1054 From: Sam Droege <sdroege@...>
Date: Thu Apr 15, 2010 7:32 pm
Subject: Re: Adobe/Bank nesting bees and building nests for such
sam_droege
Send Email Send Email
 

Hi Eric:

Good point.  It could be an issue, but it may vary by the species groups.

For example, Anthophora abrupta nests in naturally large aggregations, thus they must have some sort of mechanism to fend off parasites and other problems that come with communal living (perhaps they have new tunnels each year?).  However, nobody seems to manage for this species (though I could see the possibilities of doing so).  On the other hand Megachile and Osmia under most circumstances (but perhaps there are exceptions) are not normally aggregating species...thus the impacts on the populations of living at artificially high densities could be great and you have pointed out the problems that commercial folks have.  

I would imagine that there are others on the list who can speak to this in more detail than I can.

Vince Tepedino told me about some of Frank Parker's early work in Egypt and John Ascher told me of Jerry Rozen's work there too with S.M. Kamel on large aggregations of bees nesting in Egyptian adobe structures.  Check out:

http://www.pollinatorparadise.com/Egypt.htm

sam

                                               
Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov


Franklin Hyde, Who caroused in the Dirt and was corrected by His Uncle

His Uncle came on Franklin Hyde
Carousing in the Dirt.
He Shook him hard from Side to Side
And
Hit him till it Hurt,


Exclaiming, with a Final Thud,
'Take that! Abandoned Boy!
For Playing with Disgusting Mud
As though it were a Toy!'


Moral
From Franklin Hyde's adventure, learn
To pass your Leisure Time
In Cleanly Merriment, and turn
From Mud and Ooze and Slime
And every form of Nastiness-
But, on the other Hand,
Children in ordinary Dress
May always play with Sand.


    - Hilaire Belloc
P Bees are not optional.


From: Eric Mader <eric@...>
To: beemonitoring@yahoogroups.com
Cc: Sam Droege <sdroege@...>, rochellegreayer@...
Date: 04/15/2010 03:08 PM
Subject: Re: [beemonitoring] Adobe/Bank nesting bees and building nests for         such
Sent by: beemonitoring@yahoogroups.com





 

Interesting topic, Sam!

 

I was talking with Matthew Shepherd here in the office and we had one thought to add to this discussion.

 

What do folks think is the potential for these structures to become a population sink in their local landscape as they age and potentially become infested with chalkbrood spores and pollen mites?

 

I know from my days managing alfalfa leafcutter bees that parasites and diseases build up incredibly fast any time nesting substrates are repeatedly re-used by solitary bees.

 

On the one hand I think a certain level of pathogens and nest parasitism can be interesting from an educational standpoint, and can really engage people in the complexities of bee life. On the other hand, I have seen old nest blocks that are so contaminated that no new eggs deposited in them survive. Such a scenario seems likely to discourage rather than engage the general public if these types of walls are on formal display.

 

For large scale leafcutter and mason beekeeping most people have moved to loose-cell management or elaborate nest phase out systems to maintain bee health. With fixed substrates, arranged in these beautiful and artistic configurations, that seems like a challenge.

 

Anyone have thoughts on this?

 

Cheers!

 

-Eric



On Wed, Apr 14, 2010 at 10:21 AM, Sam Droege <sdroege@...> wrote:
 


All:


Today, Shelley Small passed on the following link regarding "Insect Walls."  These essentially being elaborate and artistic structures for hole nesting species.


http://greayer.com/studiog/?p=4529

Very nicely done and some neat designs for Nature Centers to contemplate.


I e-spoke to the author, Rochelle Greayer, about the fact that some species readily nest in the walls made of adobe or earthen plasters.  In my strawbale/adobe house house I have Anthophora abrupta (hundreds), Anthophora plumipes (introduced and unfortunately increasing), Ptilothrix bombiformis (small numbers), Melitoma taurea (small numbers), and numerous Osmia, Megachile, Chrysidid, micro-hymenoptera hangers on nesting.  


She would be interested in putting up a bit more about bees that live in adobe structures, earthen walls, or, better yet, structures of earth that people have made for these species.  So, if you have any examples, stories, or observations from around the world please share (you can post to me or to the list...I think it is of general enough interest that it would be good to have archived).


Thanks, as always.


sam


                                               
Sam Droege  
sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705

Http://www.pwrc.usgs.gov

I rose from marsh mud


I rose from marsh mud,
algae, equisetum, willows,
sweet green, noisy
birds and frogs


to see her wed in the rich
rich silence of the church,
the little white slave-girl
in her diamond fronds.


In aisle and arch
the satin secret collects.
United for life to serve
silver. Possessed.


      -Lorine Niedecker






P
Bees are not optional.



--
----------------------------------------------------------------------
Eric Mader
National Pollinator Outreach Coordinator
The Xerces Society for Invertebrate Conservation
Tel: 503-232-6639 Fax: 503-233-6794
Email:
eric@...
Skype: eric_mader_xerces_society

Assistant Professor of Extension
University of Minnesota - Department of Entomology
Email:
made0002@...

The Xerces Society is an international nonprofit organization that protects wildlife through the conservation of invertebrates and their habitat. Our Pollinator Conservation Program works to support the sustainability and profitability of farms while protecting pollinator insects. To join the Society, make a contribution, or read about our work, please visit
www.xerces.org.

Find all the information you need to conserve pollinator habitat at:

http://www.xerces.org/pollinator-resource-center/
----------------------------------------------------------------------




#1055 From: Laurence Packer <laurencepacker@...>
Date: Thu Apr 15, 2010 8:05 pm
Subject: Re: Adobe/Bank nesting bees and building nests for such
laurencepacker@...
Send Email Send Email
 
Howdy

While I know of no long term study of such walls, ground-nesting bees have been known to nest at great density in the same patch of dirt for maybe a century.  Though even for them population crashes, apparently due to the build up of macro-organisms such as cuckoo bees and velvet ants, have been recorded.  The best paper I am aware of is:

Knerer, Gerd. “Periodizität und Strategie der Schmarotzer einer
sozialen Schmalbiene, Evylaeus malachurus (K.) (Apoidea: Halictidae).”
Zoologische Anzeiger 190 (1973): 41–63.

but is in German.  I had someone read it into a tape recorder for me in 1976, but the technology wouldn't work any more even if I hadn't lost the tape!

cheers

laurence


From: Sam Droege <sdroege@...>
To: Eric Mader <eric@...>
Cc: beemonitoring@yahoogroups.com; rochellegreayer@...
Sent: Thu, April 15, 2010 3:32:24 PM
Subject: Re: [beemonitoring] Adobe/Bank nesting bees and building nests for such

 


Hi Eric:

Good point.  It could be an issue, but it may vary by the species groups.

For example, Anthophora abrupta nests in naturally large aggregations, thus they must have some sort of mechanism to fend off parasites and other problems that come with communal living (perhaps they have new tunnels each year?).  However, nobody seems to manage for this species (though I could see the possibilities of doing so).  On the other hand Megachile and Osmia under most circumstances (but perhaps there are exceptions) are not normally aggregating species...thus the impacts on the populations of living at artificially high densities could be great and you have pointed out the problems that commercial folks have.  

I would imagine that there are others on the list who can speak to this in more detail than I can.

Vince Tepedino told me about some of Frank Parker's early work in Egypt and John Ascher told me of Jerry Rozen's work there too with S.M. Kamel on large aggregations of bees nesting in Egyptian adobe structures.  Check out:

http://www.pollinat orparadise. com/Egypt. htm

sam

                                               
Sam Droege  sdroege@usgs. gov                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc. usgs.gov



Franklin Hyde, Who caroused in the Dirt and was corrected by His Uncle

His Uncle came on Franklin Hyde
Carousing in the Dirt.
He Shook him hard from Side to Side
And
Hit him till it Hurt,


Exclaiming, with a Final Thud,
'Take that! Abandoned Boy!
For Playing with Disgusting Mud
As though it were a Toy!'


Moral
From Franklin Hyde's adventure, learn
To pass your Leisure Time
In Cleanly Merriment, and turn
From Mud and Ooze and Slime
And every form of Nastiness-
But, on the other Hand,
Children in ordinary Dress
May always play with Sand.


    - Hilaire Belloc
P Bees are not optional.


From: Eric Mader <eric@xerces. org>
To: beemonitoring@ yahoogroups. com
Cc: Sam Droege <sdroege@usgs. gov>, rochellegreayer@ gmail.com
Date: 04/15/2010 03:08 PM
Subject: Re: [beemonitoring] Adobe/Bank nesting bees and building nests for         such
Sent by: beemonitoring@ yahoogroups. com





 

Interesting topic, Sam!

 

I was talking with Matthew Shepherd here in the office and we had one thought to add to this discussion.

 

What do folks think is the potential for these structures to become a population sink in their local landscape as they age and potentially become infested with chalkbrood spores and pollen mites?

 

I know from my days managing alfalfa leafcutter bees that parasites and diseases build up incredibly fast any time nesting substrates are repeatedly re-used by solitary bees.

 

On the one hand I think a certain level of pathogens and nest parasitism can be interesting from an educational standpoint, and can really engage people in the complexities of bee life. On the other hand, I have seen old nest blocks that are so contaminated that no new eggs deposited in them survive. Such a scenario seems likely to discourage rather than engage the general public if these types of walls are on formal display.

 

For large scale leafcutter and mason beekeeping most people have moved to loose-cell management or elaborate nest phase out systems to maintain bee health. With fixed substrates, arranged in these beautiful and artistic configurations, that seems like a challenge.

 

Anyone have thoughts on this?

 

Cheers!

 

-Eric



On Wed, Apr 14, 2010 at 10:21 AM, Sam Droege <sdroege@usgs. gov> wrote:
 


All:


Today, Shelley Small passed on the following link regarding "Insect Walls."  These essentially being elaborate and artistic structures for hole nesting species.


http://greayer. com/studiog/ ?p=4529

Very nicely done and some neat designs for Nature Centers to contemplate.


I e-spoke to the author, Rochelle Greayer, about the fact that some species readily nest in the walls made of adobe or earthen plasters.  In my strawbale/adobe house house I have Anthophora abrupta (hundreds), Anthophora plumipes (introduced and unfortunately increasing), Ptilothrix bombiformis (small numbers), Melitoma taurea (small numbers), and numerous Osmia, Megachile, Chrysidid, micro-hymenoptera hangers on nesting.  


She would be interested in putting up a bit more about bees that live in adobe structures, earthen walls, or, better yet, structures of earth that people have made for these species.  So, if you have any examples, stories, or observations from around the world please share (you can post to me or to the list...I think it is of general enough interest that it would be good to have archived).


Thanks, as always.


sam


                                               
Sam Droege  
sdroege@usgs. gov                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705

Http://www.pwrc. usgs.gov

I rose from marsh mud


I rose from marsh mud,
algae, equisetum, willows,
sweet green, noisy
birds and frogs


to see her wed in the rich
rich silence of the church,
the little white slave-girl
in her diamond fronds.


In aisle and arch
the satin secret collects.
United for life to serve
silver. Possessed.


      -Lorine Niedecker






P
Bees are not optional.



--
------------ --------- --------- --------- --------- --------- --------- ----
Eric Mader
National Pollinator Outreach Coordinator
The Xerces Society for Invertebrate Conservation
Tel: 503-232-6639 Fax: 503-233-6794
Email:
eric@xerces. org
Skype: eric_mader_xerces_ society

Assistant Professor of Extension
University of Minnesota - Department of Entomology
Email:
made0002@umn. edu

The Xerces Society is an international nonprofit organization that protects wildlife through the conservation of invertebrates and their habitat. Our Pollinator Conservation Program works to support the sustainability and profitability of farms while protecting pollinator insects. To join the Society, make a contribution, or read about our work, please visit
www.xerces.org.

Find all the information you need to conserve pollinator habitat at:

http://www.xerces. org/pollinator- resource- center/
------------ --------- --------- --------- --------- --------- --------- ----




__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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#1056 From: "Cane, Jim" <Jim.Cane@...>
Date: Fri Apr 16, 2010 3:07 pm
Subject: RE: aggregating Megachile and nest site longevities
Jim.Cane@...
Send Email Send Email
 

Folks- in fact Osmia and Megachile do aggregate on occasions, but typically the ground-nesting species (or any for cavity nesters, if available holes are also aggregated).  Attached is a paper reporting a case of the former. 

 

As to longevity of nesting aggregations, the longest yet known is ˝ century, with a summary in this other paper of mine on alkali bees (which rounds up both published and validated pers comm. sources).

 

In the event that the attachments don’t post, Sam and I will work out a way for you to get them.

 

jim

 

===============================

James H. Cane

USDA-ARS Bee Biology and Systematics Lab

Utah State University, Logan, UT 84322 USA

tel: 435-797-3879   FAX: 435-797-0461

email: Jim.Cane@... 

http://www.ars.usda.gov/npa/logan/beelab

http://www.biology.usu.edu/people/facultyinfo.asp?username=jcane

Gardening for Native Bees: http://extension.usu.edu/files/publications/factsheet/plants-pollinators09.pdf

 

"The obscure takes time to see,

but the obvious takes longer"
Edward R. Murrow

 


2 of 2 File(s)


#1057 From: Sam Droege <sdroege@...>
Date: Fri Apr 16, 2010 9:12 pm
Subject: BIML updates - Lasioglossum, Megachile, Melitoma, Colletes, Triepeolus, Melissodes, Andrena
sam_droege
Send Email Send Email
 

USGS Native Bee Inventory and Monitoring Laboratory Update


New State Records

Lasioglossum reticulatum – Lindsey Fenner from Savannah River Plant
Melitoma taurea – Expanding its range northward into Pennsylvania collected by Alex Surcica near Chambersburg
Triepeolus atlanticus – Lindsey Fenner from Savannah River Plant

Colletes Guide Updates

Male - C. brimleyi vs C. distinctus

C. brimleyi - Malar space about half to two-thirds as long as the width of the base of the mandibles - Hairs basad of the white apical hair band on T3 are dark colored - The long plumose hairs that line the rim of S3 are relatively uniform in height varying little across the rim except decreasing somewhat in the center - Pits on T2 mostly not touching

C. distinctus - Malar space as long or a bit longer than the width of the base of the mandibles - Hairs basad of the white apical hair band on T3 are light colored - The long plumose hairs that line the rim of S3 vary DRAMATICALLY in height across the rim, increasing from the edges to a point and then decreasing to a minute, to near absent, size in the center, thus the overall pattern looks like two large lateral triangles of hair emerging from the rim - Pits on T2 mostly touching

Melissodes Guide Updates - Mike Arduser send a note (and specimens) that M. tuckeri had been recorded from IL and thus we now include it in the guide.

Female - M. coloradensis, M. tuckeri, M. vernoniae

M. coloradensis - UNIQUE in that the wings are distinctly and unmistakably DARK OR INFUSCATED - The wide depressed rim of T2 is extensively, densely, and unmistakably invaded in the central portion by pitting, at In direct comparison, a  much LARGER bee

M. tuckeri - Wings clear, without any coloration - The wide depressed rim of T2 is extensively, densely, and unmistakably invaded in the central portion by pitting - T4 with a distinct white, latitudinal hair band behind which, or basally, is a band of black hairs that is clearly visible even when T3 overlaps T4 extensively

M. vernoniae - Wings clear, without any coloration - The wide depressed rim of T2 is essentially WITHOUT PITS, at most there are a few obscure, difficult to see pits on the far sides - The central portion of T4 almost entirely covered with white hairs with black hairs present only scattered along the very basal edge and therefore often covered by the rim of T3 and not visible
       

Andrena Guide Updates

Female - A. asteris and A. placata vs A. duplicata

A. asteris and A. placata - Mesepisturnum unpitted - Scopal hairs so dense as to completely hide the tibial integument from view - S2-4 in addition to the long hairs along the rim, with short but clearly visible hairs throughout the segment

A. duplicata - Mesepisturnum with distinct pits - Integument clearly visible beneath the scopal hairs on hind tibia - Hairs on S2-4 almost entirely restricted to scattered long hairs along the rim - In comparison, clypeus somewhat flattened in the center, and unpitted center line of clypeus wider

Megachile Guide Updates

Female - M. melanophaea, M. mucida, M. gemula

M. gemula - The last tooth or what is really just the rearmost portion of the inner edge of the mandible between the corner and the last sinus or gap is broadly truncate or flattened, not toothlike at all, in distance almost as wide as that of the sinus preceding it - Most of T2 with pale hairs, but dark brown hairs usually line the rim - Hairs on the gena or cheek nearest the mandible distinctly pale and there are some pale hairs lining the outside edges of the front coxae - Generally northern and Appalachian in distribution

M. melanophaea - The last or rearmost tooth of the mandible comes to a rounded point, but is definitely not flattened or truncate - T2 hair entirely pale - Hairs on the cheek or gena and on the front coxae entirely dark brown - Unlike the other two species this species almost always has bright orange hairs present on S2-5, but note that in some individuals these hairs are restricted and in others they may be entirely dark like the other species - Northern in distribution.

M. mucida - The last or rearmost tooth of the mandible comes to a rounded point, but is definitely not flattened or truncate - Most of T2 with pale hairs, but dark hairs usually line the rim and usually expand laterally for for wider patches of dark brown hairs - Hairs on the cheek or gena and on the front coxae entirely dark brown - Southern in distribution

Male - M. gemula, M. melanophaea, M. mucida

M. gemula - T7, which is a small segment and only made visible by turning the specimen upside down, has no projecting central spine, but there may be some small bumps present - T6 with small triangular angles projecting from the rim just on either side of the central notch, note that this is the true rim of T6 do not mistake the very prominent flange that sits above it and hides it, you will have to turn the specimen over to see it - Front basitarsi almost entirely dark brown to black, there may be some yellow along the edge - Generally northern and Appalachian in distribution

M. melanophaea - T7, which is a small segment and only made visible by turning the specimen upside down, has a prominent and clearly projecting central spine - T6 with very long, almost spine-like projecting finger like lobes projecting from the rim just on either side of the central notch, note that this is the true rim of T6 do not mistake the very prominent flange that sits above it and hides it, you will have to turn the specimen over to see it - Front basitarsi almost entirely yellow - Northern in distribution

M. mucida - T7, which is a small segment and only made visible by turning the specimen upside down, has a raised broad, blunt, triangular mound projecting from the center - T6 with small triangular angles projecting from the rim just on either side of the central notch, note that this is the true rim of T6 do not mistake the very prominent flange that sits above it and hides it, you will have to turn the specimen over to see it - Front basitarsi largely yellow - Southern in distribution

Note on Andrena duplicata in the Smokies

Adriean Mayor, curator at Great Smokies National Park send in the following regarding some recent collections of the rarely seen A. duplicate:

“An interesting sidebar with regard to the Helianthus angustifolius in Cades
Cove is that this is apparently a relict population of a coastal plain
species. I did notice that LaBerge did have a few additional records for A.
duplicata, and mentions several species of Helianthus as floral sources. It
was collected at the same time as other Fall species; A. accepta, A.
aliciae, and A. simplex. “

sam

                                             
Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

Two Views on the Same Subject

Hui Tzu said to Chuang Tzu:  "I have a big stinktree in my garden.   The trunk is so bent and knotty that nobody can get a good straight plank out of it.
The branches are so crooked you can't cut them up in any way that makes sense.  There it stands beside the road and no carpenter will even look at it.  
Such is your teaching, Chuang - big and useless."
Chuang Tzu replied: "Have you ever watched the wildcat crouching, watching its prey?   This way it leaps, and that way,
high and low, and at last - it lands in the trap.  Have you ever seen the yak?   It is great as a thundercloud, standing in his might.
Big?  Sure.  But, he can't catch mice!  So for your big tree.  No use?   Then plant it in the wasteland - in emptiness.  Walk idly around it and rest under
it's shadow.  No axe or saw prepares its end.  No one will ever cut it down.   Useless?  You should worry!.
-  Chuang Tzu, The Useless Tree, circa 200 B.C..  


If trees could scream, would we be so cavalier about cutting them down?
We might, if they screamed all the time, for no good reason.
      -   Jack Handey


#1058 From: Sam Droege <sdroege@...>
Date: Sat Apr 17, 2010 8:43 pm
Subject: July 30-August 2 Acadia National Park Hymenoptera BioBlitz
sam_droege
Send Email Send Email
 

All:

I will be up at Acadia helping with the BioBlitz and want to encourage you to come and here is why.  

1.  What an opportunity to learn first hand about all the facets of Hymenoptera that you have only read about in books and on listservs like this.
2.  We will have all sorts of scopes, nets, traps, lights, gizmos, and processing tools so if you are new to the field, here is a way to ramp up your projects by working with us and stealing our ideas, we are friendly and will be glad to show you how.
3.  As an entomologist you rarely get an opportunity to support your National Parks....here is your chance.
4.  You will never again have such a cheap and fun vacation on the Maine Coast in your life.

sam droege



Announcement is below and a there is a link to their web site and the registration forms.

2010 BioBlitz - Hymenoptera


The National Park Service, US Geological Survey, Maine Forest Service, Maine Entomological Society, University of Maine, and Acadia Partners for Science and Learning are pleased to sponsor the 8th annual bioblitz at Acadia National Park on July 30, 31, August 1 and 2. This year, we will be targeting one of the largest orders of insects - sawflies, wasps, bees, and ants.  The event is open to professional entomologists, amateur naturalists, and other interested persons.

As in the past, the event will be based at the park’s Schoodic Education and Research Center and collecting will be focused in the Schoodic section of the park.  Lodging at the Schoodic Education and Research Center will be provided to participants at no charge; however space is limited, and will is available on a first-registered, first-served basis.  Participants will only need to pay a small registration fee and food costs - meals will be provided by Acadia Partners for Science and Learning.

The event will begin with dinner on Friday evening followed by presentations about ongoing research or emerging issues of interest to the entomological community. Saturday morning will feature a workshop on collecting and identifying the Hymenoptera. The official bioBlitz will commence around noon and continue 24 hours till noon on Sunday. The remainder of Sunday and Monday morning will be focused with sorting, pinning, and identifying collected specimens.

Lead taxonomists for the event will be Sam Droege from the US Geological Survey and Eleanor Groden and Frank Drummond from the University of Maine.

To register for the blitz, please complete the attached form. The final date for registration is June 18, 2010. Return registration forms to: June Devisfruto, Acadia National Park,Schoodic Education and Research Center, P.O Box 570, Winter Harbor, ME 04693

Information and the registration form are also posted at the park’s web site: http://www.nps.gov/acad/naturescience/bioblitz.htm

For more information, please contact David Manski at Acadia National Park (david_manski@... or 207/288-8720).


#1059 From: Sam Droege <sdroege@...>
Date: Thu Apr 22, 2010 11:02 am
Subject: July Bee Species ID Course Now Open
sam_droege
Send Email Send Email
 

All:

Below is an announcement for such a course which will be held at the National Conservation Training Center in West Virginia on the week of July 5th (I know bad timing, but that's the only slot we could get for the nice lab).

You can read about the details in the announcement, but one thing to point out is that unlike in the past we will be emphasizing the details of identifying the trickier groups of species and will not cover the basics.  We are going to assume that you have seen our online identification materials, have access to a dissecting microscope at home or the office, and have been learning and practicing on known specimens of bees (which we are happy to send you).  You will learn far more about bee identification if you have taken these steps and already know your way around guides, bees, their basic body parts and have struggled with the keys.  Our job is to help end those struggles not simply start them!


Let me know right away if you plan to attend as it is strictly a first come/first serve basis, classes have always filled with long waiting lists and we make no judgements or adjustments to who attends  based on your past experience, level of education,  or current needs.



 
Training
Announcement  
 
 

 

 
Native Bee Identification, Ecology, Research and Monitoring
 

 
Course Dates: July 5-10, 2008
Course Location: National Conservation Training Center, Shepherdstown, WV (http://training.fws.gov/ )
Course Leaders: Jason Gibbs, Alana Taylor, Sam Droege
Course Length: 5 days/36 hours
 
Course Description:  
The primary goal of this course is to provide participants the tools necessary to identify bees to species. To do that instructor ratios will be kept at 1 instructor to 8 participants. We will assume that students already have access to microscopes, will have read up on the basic literature on bee genera we have provided, and will have already practiced keying out bees to the genus level. We will emphasize learning to use online guides, how to identify tricky characters within groups such as Osmia, Lasioglossum, Andrena, Nomada and will be showing these characters to the class on projecting microscopes. A large collection of Eastern North American bees will be available for your use; surplus specimens will be available free for your personal collection.
 
In conjunction with learning identification skills there will be a daily lectures on bee natural history, monitoring, and research techniques. We will be going out in the field throughout the week to set traps and net bees so that participants can see the entire spectrum of field to microscope work. We encourage participants to be bring a net, we will provide you with traps and processing equipment. You are also encouraged to bring your own specimens with you and we can help with identification, as time permits. Laptops, microscopes, will be provided.
 
Who Should Attend: Federal, state, county and municipal agencies, private consulting firms, citizen volunteers, neighborhood associations, environmental organizations, and teachers, performing native pollinator assessments or monitoring programs; with a desire to improve their identification skills.
 
How to apply: To register, email Sam Droege ( sdroege@... ). First come, first serve
 
Cost: Tuition is waived for FWS employees; for the remainder tuition is $250 U.S. Food is available at the training center and lodging is available in nearby Shepherdstown.
 
Questions: Please contact Sam Droege, sdroege@..., USGS Patuxent Wildlife Research Center
 Thanks

Sam Droege

Jason Gibbs

Alana Taylor


Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705

Http://www.pwrc.usgs.gov


this is the garden:colours come and go,

this is the garden:colours come and go,
frail azures fluttering from night's outer wing
strong silent greens silently lingering,
absolute lights like baths of golden snow.
This is the garden:pursed lips do blow
upon cool flutes within wide glooms,and sing
(of harps celestial to the quivering string)
invisible faces hauntingly and slow.


This is the garden. Time shall surely reap
and on Death's blade lie many a flower curled,
in other lands where other songs be sung;
yet stand They here enraptured,as among
the slow deep trees perpetual of sleep
some silver-fingered fountain steals the world.

    - by: e.e. cummings




P Bees are not optional.

#1060 From: <abennett@...>
Date: Mon Apr 26, 2010 8:48 pm
Subject: shipping insect samples
abennett@...
Send Email Send Email
 
All:

Does anyone have experience shipping insect samples in 70% alcohol? I have
contacted the US postal service for shipping rules and regulations but have
gotten a different answer on how to ship these samples each time.

Does anyone know how insect samples in alcohol should be packaged and shipped?

Thanks
Ashley

#1061 From: Doug Yanega <dyanega@...>
Date: Mon Apr 26, 2010 9:23 pm
Subject: Re: shipping insect samples
dyanega@...
Send Email Send Email
 
This following message contains some valuable information, as do the attached files. Web searches on "49 CFR 173.4" can supplement this, but note that some online material is already outdated.

PLEASE NOTE that this only applies to purely domestic mailings; if specimens are sent to or from any other countries, an entirely different additional set of regulations come into play. Most of which are too bizarre for words.

Subject: [NHCOLL-L:4576] More dangerous goods updates
Date: Wed, 28 Oct 2009 09:32:15 -0500
From: "Bentley, Andrew Charles" <abentley@...>
To: <NHCOLL-L@...>
Reply-To: abentley@...
Sender: owner-nhcoll-l@...

Hi all

It has just come to my attention that DOT has amended their 49 CFR 173.4 regulations in two major ways that have implications for our shipping policies:

1.      They have added section 173.4b "De Minimus Exceptions" (latest e-CFR attached) which now excludes very small quantities of hazardous materials (in our case under 1ml of ethanol) from the regulations.  The implications of this are that if you can keep tissues, for instance, to under 1ml of fluid (and under 100ml total per package) then there are NO dangerous goods requirements for the package.  This has limited application but is something to keep in mind.
2.      They have amended 173.4 to now follow IATA labeling criteria (latest e-CFR attached).  You are now required to use the red hatched label (as per IATA) for DOT (domestic) packages in place of the "This package conforms to 49 CFR 173.4".  However, it appears as if USPS has yet to adopt these changes (may just be that they are yet to update their Publication 52).  I have emailed my contacts at USPS for clarification and will let you know as soon as I hear.  Until then, we should continue to use "This package conforms to 49 CFR 173.4".  However, if you are sending packages domestically using FedEx or UPS then you are required to use the new labeling protocols.
 
As an aside, I recently received confirmation from my IATA contacts that we have been successful in our bid to have verbiage inserted into the IATA dangerous good manual (Section 2.7) exempting specimen packages from the regulations if packed according to 2.7.  The exact verbiage is yet to be drafted but will be along the lines of:
"Axxx                Museum specimens, such as specimens of mammals, birds, amphibians and reptiles, fishes, insects and other invertebrates containing small quantities of UN 1170, UN 1987 or UN 1219 are not subject to these Instructions provided the following packing and marking requirements are met:
Specimens are:
1.       Wrapped in paper towel and/or cheesecloth moistened with alcohol or an alcohol solution and then placed in a plastic bag that is heat-sealed. Any free liquid in the bag must not exceed 30 mL; or
2.       Placed in vials or other rigid containers with no more than 30 mL of alcohol or an alcohol solution;
a)      The prepared specimens are then placed in a plastic bag that is then heat-sealed;
b)      The bagged specimens are then placed inside another plastic bag with absorbent material then heat sealed;
c)      The finished bag is then placed in a strong outer packaging with suitable cushioning material;
d)      The completed package is marked "scientific research specimens, not restricted special provision Axxx applies".
The words "not restricted" and the special provision Axxx must be provided on the air waybill when an air waybill is issued.
This will only come into effect in January 2011 but will ensure that, together with the DOT and USPS letters of interpretation that we have already received (attached), all packages, whether domestic or international, will no longer fall into the category of dangerous goods.  It will also allow 30ml of free liquid per RIGID internal package (insects and other fragile specimens can be sent in free liquid) and will also allow 95% ethanol (again, up to 30ml free liquid per RIGID internal package). 

This has far reaching implications - no training necessary (you can self train to pack), previously inaccessible countries will now be accessible, international mailing by USPS to select countries (based on the USPS International Mailing Manual), no labeling requirements, 30ml of free liquid per internal package, 95% ethanol acceptable etc.  It is my hope that this will ease the burden on the small to medium sized museums as well as the larger institutions both within the US and internationally (as most countries follow IATA with some exceptions).  This has been a long and arduous process of negotiation, started with our Dangerous Goods roundtable held in Oklahoma, but the light at the end of the tunnel is now blazing!!!

Any questions please let me know.

Andy Bentley
Ichthyology Collection Manager/Specify Usability Lead
University of Kansas
Natural History Museum & Biodiversity Research Center
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA

I can give some pertinent information in a nutshell:

*IF* your shipment does NOT fall within the de minimus exceptions, then ONLY a person certified to pack and ship hazardous materials is legally allowed to send ANY volume of ethanol above the "100ml total" amount via domestic mail services. Part of the training and certification for packing and shipping hazardous materials involves the proper labeling protocols. Anyone who packs and ships specimens in ethanol themselves, without the proper labels, runs a small but very serious risk - the penalties for violating these regulations are potentially devastating. It has been years since I've shipped any specimens myself, accordingly - my institution has a shipping and receiving department, and THEY get to do the actual shipping. That's *their* job, and I could lose *mine* if I didn't comply.

It isn't surprising that a USPS employee won't know about these regulations, as they are certainly not part of the average USPS employee's daily routine, and require some pretty specialized knowledge just to FIND them.

I will also point out that propylene glycol will work as a substitute for ethanol (at temperatures above freezing, it is equal to or better than ethanol for DNA preservation). PG is a food additive, non-toxic and perfectly legal to transport, even on an airplane, as long as it's below the "number of fluid ounces" limit - I believe it's 3 ounces for carry-on, 16 for checked. It's not that easy to obtain, at least not food-grade PG (there are antifreezes based on PG, but they contain other chemicals), but an industrial-sized container will last a very long time. We invested in one such drum of PG, and it has, subsequently, greatly facilitated various foreign collecting trips that involved malaise and YPT samples. PG is, not surprisingly, solvent in ethanol, so transferring samples into EtOH after shipping is trivial.

Sincerely,

#1062 From: Laurence Packer <laurencepacker@...>
Date: Mon Apr 26, 2010 10:49 pm
Subject: Re: shipping insect samples
laurencepacker@...
Send Email Send Email
 
Note that propylene glycol has been found to be not so good for DNA sequencing material storage - at least not long term.
I know of no experiments looking at ethanol storage for a long time, then pg for brief mailing and then back into ethanol asap at the point of receipt.
It might be worth experimenting on that.

laurence



From: Doug Yanega <dyanega@...>
To: beemonitoring@yahoogroups.com
Sent: Mon, April 26, 2010 5:23:51 PM
Subject: Re: [beemonitoring] shipping insect samples

 

This following message contains some valuable information, as do the attached files. Web searches on "49 CFR 173.4" can supplement this, but note that some online material is already outdated.

PLEASE NOTE that this only applies to purely domestic mailings; if specimens are sent to or from any other countries, an entirely different additional set of regulations come into play. Most of which are too bizarre for words.

Subject: [NHCOLL-L:4576] More dangerous goods updates
Date: Wed, 28 Oct 2009 09:32:15 -0500
From: "Bentley, Andrew Charles" <abentley@ku. edu>
To: <NHCOLL-L@lists. yale.edu>
Reply-To: abentley@ku. edu
Sender: owner-nhcoll- l@.... edu

Hi all

It has just come to my attention that DOT has amended their 49 CFR 173.4 regulations in two major ways that have implications for our shipping policies:

1.       They have added section 173.4b "De Minimus Exceptions" (latest e-CFR attached) which now excludes very small quantities of hazardous materials (in our case under 1ml of ethanol) from the regulations.  The implications of this are that if you can keep tissues, for instance, to under 1ml of fluid (and under 100ml total per package) then there are NO dangerous goods requirements for the package.  This has limited application but is something to keep in mind.
2.       They have amended 173.4 to now follow IATA labeling criteria (latest e-CFR attached).  You are now required to use the red hatched label (as per IATA) for DOT (domestic) packages in place of the "This package conforms to 49 CFR 173.4".  However, it appears as if USPS has yet to adopt these changes (may just be that they are yet to update their Publication 52).  I have emailed my contacts at USPS for clarification and will let you know as soon as I hear.  Until then, we should continue to use "This package conforms to 49 CFR 173.4".  However, if you are sending packages domestically using FedEx or UPS then you are required to use the new labeling protocols.
 
As an aside, I recently received confirmation from my IATA contacts that we have been successful in our bid to have verbiage inserted into the IATA dangerous good manual (Section 2.7) exempting specimen packages from the regulations if packed according to 2.7.  The exact verbiage is yet to be drafted but will be along the lines of:
"Axxx                Museum specimens, such as specimens of mammals, birds, amphibians and reptiles, fishes, insects and other invertebrates containing small quantities of UN 1170, UN 1987 or UN 1219 are not subject to these Instructions provided the following packing and marking requirements are met:
Specimens are:
1.       Wrapped in paper towel and/or cheesecloth moistened with alcohol or an alcohol solution and then placed in a plastic bag that is heat-sealed. Any free liquid in the bag must not exceed 30 mL; or
2.       Placed in vials or other rigid containers with no more than 30 mL of alcohol or an alcohol solution;
a)      The prepared specimens are then placed in a plastic bag that is then heat-sealed;
b)      The bagged specimens are then placed inside another plastic bag with absorbent material then heat sealed;
c)      The finished bag is then placed in a strong outer packaging with suitable cushioning material;
d)      The completed package is marked "scientific research specimens, not restricted special provision Axxx applies".
The words "not restricted" and the special provision Axxx must be provided on the air waybill when an air waybill is issued.
This will only come into effect in January 2011 but will ensure that, together with the DOT and USPS letters of interpretation that we have already received (attached), all packages, whether domestic or international, will no longer fall into the category of dangerous goods.  It will also allow 30ml of free liquid per RIGID internal package (insects and other fragile specimens can be sent in free liquid) and will also allow 95% ethanol (again, up to 30ml free liquid per RIGID internal package). 

This has far reaching implications - no training necessary (you can self train to pack), previously inaccessible countries will now be accessible, international mailing by USPS to select countries (based on the USPS International Mailing Manual), no labeling requirements, 30ml of free liquid per internal package, 95% ethanol acceptable etc.  It is my hope that this will ease the burden on the small to medium sized museums as well as the larger institutions both within the US and internationally (as most countries follow IATA with some exceptions).  This has been a long and arduous process of negotiation, started with our Dangerous Goods roundtable held in Oklahoma, but the light at the end of the tunnel is now blazing!!!

Any questions please let me know.

Andy Bentley
Ichthyology Collection Manager/Specify Usability Lead
University of Kansas
Natural History Museum & Biodiversity Research Center
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA

I can give some pertinent information in a nutshell:

*IF* your shipment does NOT fall within the de minimus exceptions, then ONLY a person certified to pack and ship hazardous materials is legally allowed to send ANY volume of ethanol above the "100ml total" amount via domestic mail services. Part of the training and certification for packing and shipping hazardous materials involves the proper labeling protocols. Anyone who packs and ships specimens in ethanol themselves, without the proper labels, runs a small but very serious risk - the penalties for violating these regulations are potentially devastating. It has been years since I've shipped any specimens myself, accordingly - my institution has a shipping and receiving department, and THEY get to do the actual shipping. That's *their* job, and I could lose *mine* if I didn't comply.

It isn't surprising that a USPS employee won't know about these regulations, as they are certainly not part of the average USPS employee's daily routine, and require some pretty specialized knowledge just to FIND them.

I will also point out that propylene glycol will work as a substitute for ethanol (at temperatures above freezing, it is equal to or better than ethanol for DNA preservation) . PG is a food additive, non-toxic and perfectly legal to transport, even on an airplane, as long as it's below the "number of fluid ounces" limit - I believe it's 3 ounces for carry-on, 16 for checked. It's not that easy to obtain, at least not food-grade PG (there are antifreezes based on PG, but they contain other chemicals), but an industrial-sized container will last a very long time. We invested in one such drum of PG, and it has, subsequently, greatly facilitated various foreign collecting trips that involved malaise and YPT samples. PG is, not surprisingly, solvent in ethanol, so transferring samples into EtOH after shipping is trivial.

Sincerely,


#1063 From: Doug Yanega <dyanega@...>
Date: Mon Apr 26, 2010 11:20 pm
Subject: Re: shipping insect samples
dyanega@...
Send Email Send Email
 
Laurence wrote:

>Note that propylene glycol has been found to be not so good for DNA
>sequencing material storage - at least not long term.

Is there a published source for this? The original study was in
Invertebrate Systematics 19: 99-104 (2005), and compared various
preservatives over 6 weeks, with propylene glycol demonstrably
superior to ethanol at temperatures above freezing. This study also
cited a honeybee DNA project (Texas J. Science 55: 159-168, 2003) as
having similarly good results with PG in aerial pitfall traps. Any
contrary experimental data, or data on storage in PG exceeding six
weeks, would certainly be noteworthy. We don't use PG as a storage
medium, and it would be good to know if there is explicit reason NOT
to do so, should the question arise.

Peace,
--

Doug Yanega        Dept. of Entomology         Entomology Research Museum
Univ. of California, Riverside, CA 92521-0314        skype: dyanega
phone: (951) 827-4315 (standard disclaimer: opinions are mine, not UCR's)
               http://cache.ucr.edu/~heraty/yanega.html
    "There are some enterprises in which a careful disorderliness
          is the true method" - Herman Melville, Moby Dick, Chap. 82

#1064 From: Laurence Packer <laurencepacker@...>
Date: Mon Apr 26, 2010 11:30 pm
Subject: Re: shipping insect samples
laurencepacker@...
Send Email Send Email
 
Bryan Danforth tested some of my samples - paired tests with one bee in ethanol and one in pg at the same moment in time - mostly very small bees, mostly poor results with pg.
The barcoders have told me that pg does not work very well, and their methods are usually fairly good at getting that dna fragment.  But, not published, just stories.

laurence


From: Doug Yanega <dyanega@...>
To: beemonitoring@yahoogroups.com
Sent: Mon, April 26, 2010 7:20:25 PM
Subject: Re: [beemonitoring] shipping insect samples

 

Laurence wrote:

>Note that propylene glycol has been found to be not so good for DNA
>sequencing material storage - at least not long term.

Is there a published source for this? The original study was in
Invertebrate Systematics 19: 99-104 (2005), and compared various
preservatives over 6 weeks, with propylene glycol demonstrably
superior to ethanol at temperatures above freezing. This study also
cited a honeybee DNA project (Texas J. Science 55: 159-168, 2003) as
having similarly good results with PG in aerial pitfall traps. Any
contrary experimental data, or data on storage in PG exceeding six
weeks, would certainly be noteworthy. We don't use PG as a storage
medium, and it would be good to know if there is explicit reason NOT
to do so, should the question arise.

Peace,
--

Doug Yanega Dept. of Entomology Entomology Research Museum
Univ. of California, Riverside, CA 92521-0314 skype: dyanega
phone: (951) 827-4315 (standard disclaimer: opinions are mine, not UCR's)
http://cache. ucr.edu/~ heraty/yanega. html
"There are some enterprises in which a careful disorderliness
is the true method" - Herman Melville, Moby Dick, Chap. 82



#1065 From: "Weber, Don" <Don.Weber@...>
Date: Tue Apr 27, 2010 1:38 am
Subject: RE: shipping insect samples
Don.Weber@...
Send Email Send Email
 

For gut content DNA we had equivalent results with room temp EtOH and PG, and best results with -20C EtOH as fixative, to quantify prey marker after approx. one week of storage, see http://www.insectscience.org/9.41/ref/table1.html

However this is somewhat different than long-term storage of a whole specimen.

 

Regarding shipment, preserving in ethanol then draining the excess alcohol for an overnight shipment results in de minimus solvent and specimens fine w/r/t DNA (no citation, just several verbal reports).

 

Don

 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

 


From: beemonitoring@yahoogroups.com [mailto:beemonitoring@yahoogroups.com] On Behalf Of Laurence Packer
Sent: Monday, April 26, 2010 19:30
To: Doug Yanega; beemonitoring@yahoogroups.com
Subject: Re: [beemonitoring] shipping insect samples

 

 

Bryan Danforth tested some of my samples - paired tests with one bee in ethanol and one in pg at the same moment in time - mostly very small bees, mostly poor results with pg.
The barcoders have told me that pg does not work very well, and their methods are usually fairly good at getting that dna fragment.  But, not published, just stories.

laurence

 


From: Doug Yanega <dyanega@ucr.edu>
To: beemonitoring@yahoogroups.com
Sent: Mon, April 26, 2010 7:20:25 PM
Subject: Re: [beemonitoring] shipping insect samples

 

Laurence wrote:

>Note that propylene glycol has been found to be not so good for DNA
>sequencing material storage - at least not long term.

Is there a published source for this? The original study was in
Invertebrate Systematics 19: 99-104 (2005), and compared various
preservatives over 6 weeks, with propylene glycol demonstrably
superior to ethanol at temperatures above freezing. This study also
cited a honeybee DNA project (Texas J. Science 55: 159-168, 2003) as
having similarly good results with PG in aerial pitfall traps. Any
contrary experimental data, or data on storage in PG exceeding six
weeks, would certainly be noteworthy. We don't use PG as a storage
medium, and it would be good to know if there is explicit reason NOT
to do so, should the question arise.

Peace,
--

Doug Yanega Dept. of Entomology Entomology Research Museum
Univ. of California, Riverside, CA 92521-0314 skype: dyanega
phone: (951) 827-4315 (standard disclaimer: opinions are mine, not UCR's)
http://cache. ucr.edu/~ heraty/yanega. html
"There are some enterprises in which a careful disorderliness
is the true method" - Herman Melville, Moby Dick, Chap. 82

 


#1066 From: "Greenstone, Matt" <Matt.Greenstone@...>
Date: Tue Apr 27, 2010 4:46 pm
Subject: RE: shipping insect samples
Matt.Greenstone@...
Send Email Send Email
 

The latest wrinkle I’ve seen on this is DNAlater, a commercial compound developed for preserving RNA in specimens. It may also be safer to ship than EtOH. This comes from discussions with systematist colleaguess needing to move animals internationally.

 

Matt

 


From: beemonitoring@yahoogroups.com [mailto:beemonitoring@yahoogroups.com] On Behalf Of Laurence Packer
Sent: Monday, April 26, 2010 6:50 PM
To: Doug Yanega; beemonitoring@yahoogroups.com
Subject: Re: [beemonitoring] shipping insect samples

 

 

Note that propylene glycol has been found to be not so good for DNA sequencing material storage - at least not long term.
I know of no experiments looking at ethanol storage for a long time, then pg for brief mailing and then back into ethanol asap at the point of receipt.
It might be worth experimenting on that.

laurence

 

 


From: Doug Yanega <dyanega@ucr.edu>
To: beemonitoring@yahoogroups.com
Sent: Mon, April 26, 2010 5:23:51 PM
Subject: Re: [beemonitoring] shipping insect samples

 

This following message contains some valuable information, as do the attached files. Web searches on "49 CFR 173.4" can supplement this, but note that some online material is already outdated.

 

PLEASE NOTE that this only applies to purely domestic mailings; if specimens are sent to or from any other countries, an entirely different additional set of regulations come into play. Most of which are too bizarre for words.

 

Subject: [NHCOLL-L:4576] More dangerous goods updates

Date: Wed, 28 Oct 2009 09:32:15 -0500

From: "Bentley, Andrew Charles" <abentley@ku. edu>

To: <NHCOLL-L@lists. yale.edu>

Reply-To: abentley@ku. edu

Sender: owner-nhcoll- l@.... edu

 

Hi all

 

It has just come to my attention that DOT has amended their 49 CFR 173.4 regulations in two major ways that have implications for our shipping policies:

 

1.       They have added section 173.4b "De Minimus Exceptions" (latest e-CFR attached) which now excludes very small quantities of hazardous materials (in our case under 1ml of ethanol) from the regulations.  The implications of this are that if you can keep tissues, for instance, to under 1ml of fluid (and under 100ml total per package) then there are NO dangerous goods requirements for the package.  This has limited application but is something to keep in mind.

2.       They have amended 173.4 to now follow IATA labeling criteria (latest e-CFR attached).  You are now required to use the red hatched label (as per IATA) for DOT (domestic) packages in place of the "This package conforms to 49 CFR 173.4".  However, it appears as if USPS has yet to adopt these changes (may just be that they are yet to update their Publication 52).  I have emailed my contacts at USPS for clarification and will let you know as soon as I hear.  Until then, we should continue to use "This package conforms to 49 CFR 173.4".  However, if you are sending packages domestically using FedEx or UPS then you are required to use the new labeling protocols.

 

As an aside, I recently received confirmation from my IATA contacts that we have been successful in our bid to have verbiage inserted into the IATA dangerous good manual (Section 2.7) exempting specimen packages from the regulations if packed according to 2.7.  The exact verbiage is yet to be drafted but will be along the lines of:

"Axxx                Museum specimens, such as specimens of mammals, birds, amphibians and reptiles, fishes, insects and other invertebrates containing small quantities of UN 1170, UN 1987 or UN 1219 are not subject to these Instructions provided the following packing and marking requirements are met:

Specimens are:

1.       Wrapped in paper towel and/or cheesecloth moistened with alcohol or an alcohol solution and then placed in a plastic bag that is heat-sealed. Any free liquid in the bag must not exceed 30 mL; or

2.       Placed in vials or other rigid containers with no more than 30 mL of alcohol or an alcohol solution;

a)      The prepared specimens are then placed in a plastic bag that is then heat-sealed;

b)      The bagged specimens are then placed inside another plastic bag with absorbent material then heat sealed;

c)      The finished bag is then placed in a strong outer packaging with suitable cushioning material;

d)      The completed package is marked "scientific research specimens, not restricted special provision Axxx applies".

The words "not restricted" and the special provision Axxx must be provided on the air waybill when an air waybill is issued.

This will only come into effect in January 2011 but will ensure that, together with the DOT and USPS letters of interpretation that we have already received (attached), all packages, whether domestic or international, will no longer fall into the category of dangerous goods.  It will also allow 30ml of free liquid per RIGID internal package (insects and other fragile specimens can be sent in free liquid) and will also allow 95% ethanol (again, up to 30ml free liquid per RIGID internal package). 

 

This has far reaching implications - no training necessary (you can self train to pack), previously inaccessible countries will now be accessible, international mailing by USPS to select countries (based on the USPS International Mailing Manual), no labeling requirements, 30ml of free liquid per internal package, 95% ethanol acceptable etc.  It is my hope that this will ease the burden on the small to medium sized museums as well as the larger institutions both within the US and internationally (as most countries follow IATA with some exceptions).  This has been a long and arduous process of negotiation, started with our Dangerous Goods roundtable held in Oklahoma, but the light at the end of the tunnel is now blazing!!!

 

Any questions please let me know.

 

Andy Bentley
Ichthyology Collection Manager/Specify Usability Lead
University of Kansas
Natural History Museum & Biodiversity Research Center
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561

USA

 

I can give some pertinent information in a nutshell:

 

*IF* your shipment does NOT fall within the de minimus exceptions, then ONLY a person certified to pack and ship hazardous materials is legally allowed to send ANY volume of ethanol above the "100ml total" amount via domestic mail services. Part of the training and certification for packing and shipping hazardous materials involves the proper labeling protocols. Anyone who packs and ships specimens in ethanol themselves, without the proper labels, runs a small but very serious risk - the penalties for violating these regulations are potentially devastating. It has been years since I've shipped any specimens myself, accordingly - my institution has a shipping and receiving department, and THEY get to do the actual shipping. That's *their* job, and I could lose *mine* if I didn't comply.

 

It isn't surprising that a USPS employee won't know about these regulations, as they are certainly not part of the average USPS employee's daily routine, and require some pretty specialized knowledge just to FIND them.

 

I will also point out that propylene glycol will work as a substitute for ethanol (at temperatures above freezing, it is equal to or better than ethanol for DNA preservation) . PG is a food additive, non-toxic and perfectly legal to transport, even on an airplane, as long as it's below the "number of fluid ounces" limit - I believe it's 3 ounces for carry-on, 16 for checked. It's not that easy to obtain, at least not food-grade PG (there are antifreezes based on PG, but they contain other chemicals), but an industrial-sized container will last a very long time. We invested in one such drum of PG, and it has, subsequently, greatly facilitated various foreign collecting trips that involved malaise and YPT samples. PG is, not surprisingly, solvent in ethanol, so transferring samples into EtOH after shipping is trivial.

 

Sincerely,

 


#1067 From: H <HIkerd@...>
Date: Tue Apr 27, 2010 4:49 pm
Subject: Thoughts on bees, bowls and Xeric landscapes....
hawki7
Send Email Send Email
 
All-

Sorry about the quality of these pictures but I think you can get the idea. Here is a bit of background.

In the prior 3 days close to 10 bowl lines of 30 bowls were run [10 blue, 10 white, 10 yellow]. During this time blue bowls exhibited a paucity of catch, almost an order of magnitude less bee specimens.

Now, please view this piece of antadotal evidence [photo link below] with the knowledge that I was trying to set out bee bowls in an environment that in which there would be NO CATCH. Also note, I couldn't find a flower within a kilometer radius [as far as I walked around].

http://picasaweb.google.com/hikerd/Bee_bowls?feat=directlink

Enjoy,
H


HW Ikerd
Hikerd@...
435-227-5711 (Google Voice)
435-797-2425(work)


#1068 From: Charley Eiseman <ceiseman@...>
Date: Tue Apr 27, 2010 4:56 pm
Subject: Anthophora abrupta nest sites
charleyeiseman
Send Email Send Email
 
Hello all,

I'm hoping to visit and photograph some Anthophora abrupta nests this spring, and I was wondering if anyone knows of any colonies in the northeast.  I live in Massachusetts, and at this point the closest spot I know of where I can count on seeing them is in Maryland.

Thanks in advance for any leads!

Charley Eiseman

#1069 From: Sam Droege <sdroege@...>
Date: Tue Apr 27, 2010 5:03 pm
Subject: Re: Thoughts on bees, bowls and Xeric landscapes....
sam_droege
Send Email Send Email
 

Harold:

Very interesting.  So basically you put out 300 bowls (yes?) and what was your total bee catch across those bowls?

I will be running a similar experiment this summer with my niece (who needs a project for her program).  We have gotten permission from the Redskins to use their monster parking lots (not medians, no trees, nothing alive, just asphalt) and will be running bowls along the perimeter and then into the center of the lots towards the stadium to see what the catch differential is with distance from possible habitat.  The idea, like yours, to see what proportion of bees might simply be floating or foraging over long distances.

sam

Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

John Ruskin Considers the Nature of Water, Circa 1842
        A found poem from Ruskin's Modern Painters

Now the fact is
that there is hardly
a roadside pond or pool
which has not as much
landscape in it as above it.
It is not the dull,
muddy, brown thing
we suppose it to be;
it has a heart like ourselves,
and in the bottom of that
there are the boughs
of the tall trees, and the
blades of the shaking grass,
and all manner of hues,
of variable, pleasant light
out of the sky; nay,
the ugly gutter that stagnates
over the drain bars,
in the heart of the foul city,
is not altogether base;
down in that, if you will look
deep enough, you may see
the dark, serious blue
of far-off sky, and the passing
of pure clouds.
   - Ralph Black
P Bees are not optional.


From: H <HIkerd@...>
To: beemonitoring@yahoogroups.com
Date: 04/27/2010 12:51 PM
Subject: [beemonitoring] Thoughts on bees, bowls and Xeric landscapes....
Sent by: beemonitoring@yahoogroups.com





 

All-

Sorry about the quality of these pictures but I think you can get the idea. Here is a bit of background.

In the prior 3 days close to 10 bowl lines of 30 bowls were run [10 blue, 10 white, 10 yellow]. During this time blue bowls exhibited a paucity of catch, almost an order of magnitude less bee specimens.

Now, please view this piece of antadotal evidence [photo link below] with the knowledge that I was trying to set out bee bowls in an environment that in which there would be NO CATCH. Also note, I couldn't find a flower within a kilometer radius [as far as I walked around].

http://picasaweb.google.com/hikerd/Bee_bowls?feat=directlink

Enjoy,
H


HW Ikerd

Hikerd@...
435-227-5711 (Google Voice)
435-797-2425(work)




#1070 From: Doug Yanega <dyanega@...>
Date: Tue Apr 27, 2010 6:03 pm
Subject: Re: Thoughts on bees, bowls and Xeric landscapes....
dyanega@...
Send Email Send Email
 
If you want another spot with nothing in bloom, just try the Bristol
Dry Lake salt pan near Amboy, CA (~ 34 29 30 N, 115 44 43 W) - I
think at some points it can be almost 10 km from plants on one side
of the pan to the other, with a minimum distance of maybe 5 km (i.e.,
you can draw a circle 5 km in diameter and not have a single plant
within that circle). Still, I'm sure you could catch bees in traps
there. Where was your spot, Harold?

Peace,
--

Doug Yanega        Dept. of Entomology         Entomology Research Museum
Univ. of California, Riverside, CA 92521-0314        skype: dyanega
phone: (951) 827-4315 (standard disclaimer: opinions are mine, not UCR's)
               http://cache.ucr.edu/~heraty/yanega.html
    "There are some enterprises in which a careful disorderliness
          is the true method" - Herman Melville, Moby Dick, Chap. 82

#1071 From: H <HIkerd@...>
Date: Tue Apr 27, 2010 6:12 pm
Subject: Re: Thoughts on bees, bowls and Xeric landscapes....
hawki7
Send Email Send Email
 
Sam-

We haven't gotten everything pinned up yet, sorry. Also, I only ran 5 bowls of each color on the salt flats near the Texas-New Mexico border. Yes, there was a tangible salt crust over the sand.

And as to your study.. GREAT. I've always had this kicking around in the back of my head and just saw an opportunity when taking Terry to the Airport in El Paso. Here are two other thoughts that I may or may not have time for...

One unlike the bees from the previous days the bees in the photos [from the salt flat] were without viable pollen. In most cases the prior days bees could be seen to be caring a bright orange pollen even before bending down and picking up the bowls[assumed to be from Sophora]. Thus I would like to run bowlines in which I took high quality pictures of the bees and potential pollen loads...BEFORE losing this information during strain and transfer to the whirlpaks. Finally, the temporal aspect of the bowls... number the bowls and take pictures of the contents every 30 min. along a trap line for every bowl. 

Happy spring,
H


HW Ikerd
Hikerd@...
435-227-5711 (Google Voice)
435-797-2425(work)



On Tue, Apr 27, 2010 at 11:03 AM, Sam Droege <sdroege@...> wrote:
 


Harold:

Very interesting.  So basically you put out 300 bowls (yes?) and what was your total bee catch across those bowls?

I will be running a similar experiment this summer with my niece (who needs a project for her program).  We have gotten permission from the Redskins to use their monster parking lots (not medians, no trees, nothing alive, just asphalt) and will be running bowls along the perimeter and then into the center of the lots towards the stadium to see what the catch differential is with distance from possible habitat.  The idea, like yours, to see what proportion of bees might simply be floating or foraging over long distances.

sam

Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

John Ruskin Considers the Nature of Water, Circa 1842
        A found poem from Ruskin's Modern Painters

Now the fact is
that there is hardly
a roadside pond or pool
which has not as much
landscape in it as above it.
It is not the dull,
muddy, brown thing
we suppose it to be;
it has a heart like ourselves,
and in the bottom of that
there are the boughs
of the tall trees, and the
blades of the shaking grass,
and all manner of hues,
of variable, pleasant light
out of the sky; nay,
the ugly gutter that stagnates
over the drain bars,
in the heart of the foul city,
is not altogether base;
down in that, if you will look
deep enough, you may see
the dark, serious blue
of far-off sky, and the passing
of pure clouds.
   - Ralph Black
P Bees are not optional.


From: H <HIkerd@...>
To: beemonitoring@yahoogroups.com
Date: 04/27/2010 12:51 PM
Subject: [beemonitoring] Thoughts on bees, bowls and Xeric landscapes....
Sent by: beemonitoring@yahoogroups.com





 

All-

Sorry about the quality of these pictures but I think you can get the idea. Here is a bit of background.

In the prior 3 days close to 10 bowl lines of 30 bowls were run [10 blue, 10 white, 10 yellow]. During this time blue bowls exhibited a paucity of catch, almost an order of magnitude less bee specimens.

Now, please view this piece of antadotal evidence [photo link below] with the knowledge that I was trying to set out bee bowls in an environment that in which there would be NO CATCH. Also note, I couldn't find a flower within a kilometer radius [as far as I walked around].

http://picasaweb.google.com/hikerd/Bee_bowls?feat=directlink

Enjoy,
H


HW Ikerd

Hikerd@...
435-227-5711 (Google Voice)
435-797-2425(work)





#1072 From: Sam Droege <sdroege@...>
Date: Wed Apr 28, 2010 7:36 pm
Subject: What is the Phenological Start of the Bee Season in Western Alpine areas?
sam_droege
Send Email Send Email
 

All:

As part of a large National Park Service Project we will be surveying alpine areas from the Southern Rockies and Sierra's up into Alaska.  Since the bee season varies in alpine systems so greatly among years and localities we are trying to slice out a relatively similar phenological window to do surveys.  We are thinking that it would be useful to use some set of early season flowers as a flag to when surveys should begin.

So are questions are:

1.  Does this make sense?
2.  What set of flowers could be used that would encompass that geographic range and capture about the same window?
3.  Do you have alternative suggestions or see potential problems?

Thank you.

sam

Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov
   


Velocity Meadows

I can say now that nothing was possible
But leaving the house and standing in front of it, staring
As long as I could into the valley. I knew that a train,
Trailing a scarf of smoke, would arrive, that soon it would rain.
A frieze of clouds lowered a shadow over the town,
And a driving wind flattened the meadows that swept
Beyond the olive trees and banks of hollyhock and rose.
The air smelled sweet, and a girl was waving a stick
At some crows so far away they seemed like flies.
Her mother, wearing a cape and shawl, shielded her eyes.
I wondered from what, since there was no sun. Then someone
Appeared and said, "Look at those clouds forming a wall, those crows
Falling out of the sky, those fields, pale green, green-yellow,
Rolling away, and that girl and her mother, waving goodbye."
In a moment the sky was stained with a reddish haze,
And the person beside me was running away. It was dusk,
The lights of the town were coming on, and I saw, dimly at first,
Close to the graveyard bound by rows of cypress bending down,

The girl and her mother, next to each other,
Smoking, grinding their heels into the ground.


     - Mark Strand
               






P Bees are not optional.

#1073 From: David Inouye <inouye@...>
Date: Wed Apr 28, 2010 7:58 pm
Subject: Re: What is the Phenological Start of the Bee Season in Western Alpine areas?
dwinouye
Send Email Send Email
 
At 03:36 PM 4/28/2010, Sam Droege wrote:
 
All:

As part of a large National Park Service Project we will be surveying alpine areas from the Southern Rockies and Sierra's up into Alaska.  Since the bee season varies in alpine systems so greatly among years and localities we are trying to slice out a relatively similar phenological window to do surveys.  We are thinking that it would be useful to use some set of early season flowers as a flag to when surveys should begin.

So are questions are:

1.  Does this make sense?

Yes

2.  What set of flowers could be used that would encompass that geographic range and capture about the same window?


Mertensia oblongifolia (Nutt.) G. Don or another Mertensia species


Maybe Tetraneuris (Hymenoxys) grandiflora (Torr. & A. Gray ex A. Gray) K.F. Parker, although this is more mid-season than early. Easy to spot when it's in bloom.


3.  Do you have alternative suggestions or see potential problems?

I have data since 1973 on flowering phenology at 9,500 feet in Colorado.  But that's sub-alpine.

If you want samples from the area around Crested Butte, CO, I or others at the Rocky Mtn. Biological Lab could do some sampling. 

David


Thank you.

sam

Sam Droege  sdroege@...                     
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov
  

#1074 From: "Cheryl Fimbel" <cfimbel@...>
Date: Thu Apr 29, 2010 12:21 am
Subject: RE: What is the Phenological Start of the Bee Season in Western Alpine areas?
cfimbel@...
Send Email Send Email
 

Sam,

Snowpack varies widely among years, so yes, your approach makes sense.

Classic early season alpine and subalpine flowers are the pasqueflowers (refers to Passover and Easter, as they bloom about that time of year if not buried).  I have seen their blooms poking up through snow.  The eastern pasqueflower, Pulsatilla patens, occurs in the Rocky mountain chain.  The white pasqueflower, Pulsatilla occidentalis, replaces it further west in the Sierras and north.

 

Cheryl Fimbel

The Nature Conservancy

Olympia, WA

 

From: beemonitoring@yahoogroups.com [mailto:beemonitoring@yahoogroups.com] On Behalf Of Sam Droege
Sent: Wednesday, April 28, 2010 12:37 PM
To: beemonitoring@yahoogroups.com
Subject: [beemonitoring] What is the Phenological Start of the Bee Season in Western Alpine areas?

 

 


All:

As part of a large National Park Service Project we will be surveying alpine areas from the Southern Rockies and Sierra's up into Alaska.  Since the bee season varies in alpine systems so greatly among years and localities we are trying to slice out a relatively similar phenological window to do surveys.  We are thinking that it would be useful to use some set of early season flowers as a flag to when surveys should begin.

So are questions are:

1.  Does this make sense?
2.  What set of flowers could be used that would encompass that geographic range and capture about the same window?
3.  Do you have alternative suggestions or see potential problems?

Thank you.

sam

Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov
   



Velocity Meadows

I can say now that nothing was possible
But leaving the house and standing in front of it, staring
As long as I could into the valley. I knew that a train,
Trailing a scarf of smoke, would arrive, that soon it would rain.
A frieze of clouds lowered a shadow over the town,
And a driving wind flattened the meadows that swept
Beyond the olive trees and banks of hollyhock and rose.
The air smelled sweet, and a girl was waving a stick
At some crows so far away they seemed like flies.
Her mother, wearing a cape and shawl, shielded her eyes.
I wondered from what, since there was no sun. Then someone
Appeared and said, "Look at those clouds forming a wall, those crows
Falling out of the sky, those fields, pale green, green-yellow,
Rolling away, and that girl and her mother, waving goodbye."
In a moment the sky was stained with a reddish haze,
And the person beside me was running away. It was dusk,
The lights of the town were coming on, and I saw, dimly at first,
Close to the graveyard bound by rows of cypress bending down,

The girl and her mother, next to each other,
Smoking, grinding their heels into the ground.


     - Mark Strand
               






P Bees are not optional.


#1075 From: "John S. Ascher" <ascher@...>
Date: Thu Apr 29, 2010 2:37 am
Subject: RE: What is the Phenological Start of the Bee Season in Western Alpine areas?
limon_cocha
Send Email Send Email
 
Sam and Cheryl,

In my experience, conspicuous bee activity begins precisely when the
earliest native willows start blooming. This can happen when there are
still large patches of snow on the ground. In the mountains, it can be
warm enough at midday for bee flight (ca. 55 deg. in the sun) even when
night and average temps are still low. I've found Andrena and other bees
in considerable abundance early in spring at high alpine passes where
willow is in bloom, even though it still appeared "too early" for general
flowering and bee activity at substantially lower elevations.

Thus I recommend starting sampling on the first day with warm enough air
temps for bee flight (ca. 55 deg?) within the blooming season of Salix at
the elevation of interest (ask local botanists). Snow will likely still be
present. If you wait until spring has fully arrived and there is general
flowering you may miss Salix specialists, and in particular protandrous
males of the earliest-emerging Andrena species.

Good luck!

John


> Sam,
>
> Snowpack varies widely among years, so yes, your approach makes sense.
>
> Classic early season alpine and subalpine flowers are the pasqueflowers
> (refers to Passover and Easter, as they bloom about that time of year if
> not
> buried).  I have seen their blooms poking up through snow.  The eastern
> pasqueflower, Pulsatilla patens, occurs in the Rocky mountain chain.  The
> white pasqueflower, Pulsatilla occidentalis, replaces it further west in
> the
> Sierras and north.
>
>
>
> Cheryl Fimbel
>
> The Nature Conservancy
>
> Olympia, WA
>
>
>
> From: beemonitoring@yahoogroups.com [mailto:beemonitoring@yahoogroups.com]
> On Behalf Of Sam Droege
> Sent: Wednesday, April 28, 2010 12:37 PM
> To: beemonitoring@yahoogroups.com
> Subject: [beemonitoring] What is the Phenological Start of the Bee Season
> in
> Western Alpine areas?
>
>
>
>
>
>
> All:
>
> As part of a large National Park Service Project we will be surveying
> alpine
> areas from the Southern Rockies and Sierra's up into Alaska.  Since the
> bee
> season varies in alpine systems so greatly among years and localities we
> are
> trying to slice out a relatively similar phenological window to do
> surveys.
> We are thinking that it would be useful to use some set of early season
> flowers as a flag to when surveys should begin.
>
> So are questions are:
>
> 1.  Does this make sense?
> 2.  What set of flowers could be used that would encompass that geographic
> range and capture about the same window?
> 3.  Do you have alternative suggestions or see potential problems?
>
> Thank you.
>
> sam
>
> Sam Droege  sdroege@...
> w 301-497-5840 h 301-390-7759 fax 301-497-5624
> USGS Patuxent Wildlife Research Center
> BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
>  <Http://www.pwrc.usgs.gov/> Http://www.pwrc.usgs.gov
>
>
>
>
> Velocity Meadows
>
> I can say now that nothing was possible
> But leaving the house and standing in front of it, staring
> As long as I could into the valley. I knew that a train,
> Trailing a scarf of smoke, would arrive, that soon it would rain.
> A frieze of clouds lowered a shadow over the town,
> And a driving wind flattened the meadows that swept
> Beyond the olive trees and banks of hollyhock and rose.
> The air smelled sweet, and a girl was waving a stick
> At some crows so far away they seemed like flies.
> Her mother, wearing a cape and shawl, shielded her eyes.
> I wondered from what, since there was no sun. Then someone
> Appeared and said, "Look at those clouds forming a wall, those crows
> Falling out of the sky, those fields, pale green, green-yellow,
> Rolling away, and that girl and her mother, waving goodbye."
> In a moment the sky was stained with a reddish haze,
> And the person beside me was running away. It was dusk,
> The lights of the town were coming on, and I saw, dimly at first,
> Close to the graveyard bound by rows of cypress bending down,
> The girl and her mother, next to each other,
> Smoking, grinding their heels into the ground.
>
>      - Mark Strand
>
>
>
>
>
>
> P Bees are not optional.
>
>
>
>


--
John S. Ascher, Ph.D.
Bee Database Project Manager
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West @ 79th St.
New York, NY 10024-5192
work phone: 212-496-3447
mobile phone: 917-407-0378

#1076 From: H <HIkerd@...>
Date: Thu Apr 29, 2010 3:13 am
Subject: Re: What is the Phenological Start of the Bee Season in Western Alpine areas?
hawki7
Send Email Send Email
 
Sam-I agree with every thing that Jon just said. I will also say that fickle weather has really changed my concept of when bees will fly. This is especially true of Alpine locations - I have found bee activity around 5pm after a freak snow storm that same morning follow by a general warming trend up into the later afternoon. Only by happenstance did I have my net and was able to collect. I think that above 8000ft. all bets are off. If it is sunny and anywhere above 50 there will be something flying. Even at low temps but with sun bees can be found sunning themselves on rocks or just hanging out in flowers. With bowls, I would expect that as soon as there is snow free ground, it would be the time to start.

On another subject - heavily forested locations have also caused me to re-evaluate what I think of as collectible areas[just sub-alpine]. I've worked in very heavily canopied lodgepole pine forests where the sunlight was dappled at best YET one tree coming down opened the way for a patch of sun follow by a few herbaceous plants [ I'm thinking specifically about Senecio in this case]. Not great collecting by any means of the imagination but after sitting for lunch I counted about 10 visitations in 50 minutes. I only collected two of the osmia [the only genera seen visiting] so there had to be at lest 3 different individuals...  really makes me rethink the flight distance for the bees visiting/trap-lining such isolated plants.

Anyway - all the best,
H


HW Ikerd
Hikerd@...
435-227-5711 (Google Voice)
435-797-2425(work)



On Wed, Apr 28, 2010 at 8:37 PM, John S. Ascher <ascher@...> wrote:
 


Sam and Cheryl,

In my experience, conspicuous bee activity begins precisely when the
earliest native willows start blooming. This can happen when there are
still large patches of snow on the ground. In the mountains, it can be
warm enough at midday for bee flight (ca. 55 deg. in the sun) even when
night and average temps are still low. I've found Andrena and other bees
in considerable abundance early in spring at high alpine passes where
willow is in bloom, even though it still appeared "too early" for general
flowering and bee activity at substantially lower elevations.

Thus I recommend starting sampling on the first day with warm enough air
temps for bee flight (ca. 55 deg?) within the blooming season of Salix at
the elevation of interest (ask local botanists). Snow will likely still be
present. If you wait until spring has fully arrived and there is general
flowering you may miss Salix specialists, and in particular protandrous
males of the earliest-emerging Andrena species.

Good luck!

John



> Sam,
>
> Snowpack varies widely among years, so yes, your approach makes sense.
>
> Classic early season alpine and subalpine flowers are the pasqueflowers
> (refers to Passover and Easter, as they bloom about that time of year if
> not
> buried). I have seen their blooms poking up through snow. The eastern
> pasqueflower, Pulsatilla patens, occurs in the Rocky mountain chain. The
> white pasqueflower, Pulsatilla occidentalis, replaces it further west in
> the
> Sierras and north.
>
>
>
> Cheryl Fimbel
>
> The Nature Conservancy
>
> Olympia, WA
>
>
>
> From: beemonitoring@yahoogroups.com [mailto:beemonitoring@yahoogroups.com]
> On Behalf Of Sam Droege
> Sent: Wednesday, April 28, 2010 12:37 PM
> To: beemonitoring@yahoogroups.com
> Subject: [beemonitoring] What is the Phenological Start of the Bee Season
> in
> Western Alpine areas?
>
>
>
>
>
>
> All:
>
> As part of a large National Park Service Project we will be surveying
> alpine
> areas from the Southern Rockies and Sierra's up into Alaska. Since the
> bee
> season varies in alpine systems so greatly among years and localities we
> are
> trying to slice out a relatively similar phenological window to do
> surveys.
> We are thinking that it would be useful to use some set of early season
> flowers as a flag to when surveys should begin.
>
> So are questions are:
>
> 1. Does this make sense?
> 2. What set of flowers could be used that would encompass that geographic
> range and capture about the same window?
> 3. Do you have alternative suggestions or see potential problems?
>
> Thank you.
>
> sam
>
> Sam Droege sdroege@...
> w 301-497-5840 h 301-390-7759 fax 301-497-5624
> USGS Patuxent Wildlife Research Center
> BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD 20705
> <Http://www.pwrc.usgs.gov/> Http://www.pwrc.usgs.gov

>
>
>
>
> Velocity Meadows
>
> I can say now that nothing was possible
> But leaving the house and standing in front of it, staring
> As long as I could into the valley. I knew that a train,
> Trailing a scarf of smoke, would arrive, that soon it would rain.
> A frieze of clouds lowered a shadow over the town,
> And a driving wind flattened the meadows that swept
> Beyond the olive trees and banks of hollyhock and rose.
> The air smelled sweet, and a girl was waving a stick
> At some crows so far away they seemed like flies.
> Her mother, wearing a cape and shawl, shielded her eyes.
> I wondered from what, since there was no sun. Then someone
> Appeared and said, "Look at those clouds forming a wall, those crows
> Falling out of the sky, those fields, pale green, green-yellow,
> Rolling away, and that girl and her mother, waving goodbye."
> In a moment the sky was stained with a reddish haze,
> And the person beside me was running away. It was dusk,
> The lights of the town were coming on, and I saw, dimly at first,
> Close to the graveyard bound by rows of cypress bending down,
> The girl and her mother, next to each other,
> Smoking, grinding their heels into the ground.
>
> - Mark Strand
>
>
>
>
>
>
> P Bees are not optional.
>
>
>
>

--
John S. Ascher, Ph.D.
Bee Database Project Manager
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West @ 79th St.
New York, NY 10024-5192
work phone: 212-496-3447
mobile phone: 917-407-0378



#1077 From: David Inouye <inouye@...>
Date: Thu Apr 29, 2010 3:28 am
Subject: Re: What is the Phenological Start of the Bee Season in Western Alpine areas?
dwinouye
Send Email Send Email
 
Salix is a good idea.  There is an alpine species, but it's inconspicuous.  Also widespread throughout the West ( http://plants.usda.gov/java/nameSearch?keywordquery=salix+nivalis&mode=sciname )

It's pretty common to see Bombus queens on Salix shrubs at lower altitudes, maybe on the alpine species too.


On Wed, Apr 28, 2010 at 8:37 PM, John S. Ascher <ascher@...> wrote:
 


Sam and Cheryl,

In my experience, conspicuous bee activity begins precisely when the
earliest native willows start blooming. This can happen when there are
still large patches of snow on the ground. In the mountains, it can be
warm enough at midday for bee flight (ca. 55 deg. in the sun) even when
night and average temps are still low. I've found Andrena and other bees
in considerable abundance early in spring at high alpine passes where
willow is in bloom, even though it still appeared "too early" for general
flowering and bee activity at substantially lower elevations.

Thus I recommend starting sampling on the first day with warm enough air
temps for bee flight (ca. 55 deg?) within the blooming season of Salix at
the elevation of interest (ask local botanists). Snow will likely still be
present. If you wait until spring has fully arrived and there is general
flowering you may miss Salix specialists, and in particular protandrous
males of the earliest-emerging Andrena species.

Good luck!

John


> Sam,
>
> Snowpack varies widely among years, so yes, your approach makes sense.
>
> Classic early season alpine and subalpine flowers are the pasqueflowers
> (refers to Passover and Easter, as they bloom about that time of year if
> not
> buried). I have seen their blooms poking up through snow. The eastern
> pasqueflower, Pulsatilla patens, occurs in the Rocky mountain chain. The
> white pasqueflower, Pulsatilla occidentalis, replaces it further west in
> the
> Sierras and north.
>
>
>
> Cheryl Fimbel
>
> The Nature Conservancy
>
> Olympia, WA
>
>
>
> From: beemonitoring@yahoogroups.com [ mailto:beemonitoring@yahoogroups.com]
> On Behalf Of Sam Droege
> Sent: Wednesday, April 28, 2010 12:37 PM
> To: beemonitoring@yahoogroups.com
> Subject: [beemonitoring] What is the Phenological Start of the Bee Season
> in
> Western Alpine areas?
>
>
>
>
>
>
> All:
>
> As part of a large National Park Service Project we will be surveying
> alpine
> areas from the Southern Rockies and Sierra's up into Alaska. Since the
> bee
> season varies in alpine systems so greatly among years and localities we
> are
> trying to slice out a relatively similar phenological window to do
> surveys.
> We are thinking that it would be useful to use some set of early season
> flowers as a flag to when surveys should begin.
>
> So are questions are:
>
> 1. Does this make sense?
> 2. What set of flowers could be used that would encompass that geographic
> range and capture about the same window?
> 3. Do you have alternative suggestions or see potential problems?
>
> Thank you.
>
> sam
>
> Sam Droege sdroege@...
> w 301-497-5840 h 301-390-7759 fax 301-497-5624
> USGS Patuxent Wildlife Research Center
> BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD 20705
> <Http://www.pwrc.usgs.gov/> Http://www.pwrc.usgs.gov

>
>
>
>
> Velocity Meadows
>
> I can say now that nothing was possible
> But leaving the house and standing in front of it, staring
> As long as I could into the valley. I knew that a train,
> Trailing a scarf of smoke, would arrive, that soon it would rain.
> A frieze of clouds lowered a shadow over the town,
> And a driving wind flattened the meadows that swept
> Beyond the olive trees and banks of hollyhock and rose.
> The air smelled sweet, and a girl was waving a stick
> At some crows so far away they seemed like flies.
> Her mother, wearing a cape and shawl, shielded her eyes.
> I wondered from what, since there was no sun. Then someone
> Appeared and said, "Look at those clouds forming a wall, those crows
> Falling out of the sky, those fields, pale green, green-yellow,
> Rolling away, and that girl and her mother, waving goodbye."
> In a moment the sky was stained with a reddish haze,
> And the person beside me was running away. It was dusk,
> The lights of the town were coming on, and I saw, dimly at first,
> Close to the graveyard bound by rows of cypress bending down,
> The girl and her mother, next to each other,
> Smoking, grinding their heels into the ground.
>
> - Mark Strand
>
>
>
>
>
>
> P Bees are not optional.
>
>
>
>

--
John S. Ascher, Ph.D.
Bee Database Project Manager
Division of Invertebrate Zoology
American Museum of Natural History
Central Park West @ 79th St.
New York, NY 10024-5192
work phone: 212-496-3447
mobile phone: 917-407-0378



#1078 From: Sam Droege <sdroege@...>
Date: Fri Apr 30, 2010 1:24 pm
Subject: New BIML mappable image of bee morphology template - Ptilothrix bombiformis
sam_droege
Send Email Send Email
 

All:

Joshua Thomas (a BIML intern) has taken on of Graham Snodgrass' magnificent photos of Ptilothrix bombiformis and created a mappable diagram of that bee's morphology.

http://pick14.pick.uga.edu/cricket/Test/Bee%20Image%20Map.html

We plan to use this as a template for doing a whole series of mapped drawings (and landscapes) to help folks learn their bee id.

Josh is putting together a word document on how to set up diagrams like this for those interested in doing this themselves and we will send that out to everyone.

sam

                                               
Sam Droege  sdroege@...                      
w 301-497-5840 h 301-390-7759 fax 301-497-5624
USGS Patuxent Wildlife Research Center
BARC-EAST, BLDG 308, RM 124 10300 Balt. Ave., Beltsville, MD  20705
Http://www.pwrc.usgs.gov

My Name

 One night when the lawn was a golden green
and the marbled moonlit trees rose like fresh memorials
in the scented air, and the whole countryside pulsed
with the chirr and murmur of insects, I lay in the grass
feeling the great distances open above me, and wondered
what I would become -- and where I would find myself --
and though I barely existed, I felt for an instant
that the vast star-clustered sky was mine, and I heard
my name as if for the first time, heard it the way
one hears the wind or the rain, but faint and far off
as though it belonged not to me but to the silence
from which it had come and to which it would go.


       -- Mark Strand



P Bees are not optional.

#1079 From: "Cane, Jim" <Jim.Cane@...>
Date: Tue May 11, 2010 6:53 pm
Subject: John Free's great crop pollination book now on-line
Jim.Cane@...
Send Email Send Email
 

Folks- The masterful book "Insect Pollination of Crops" has been
scanned and is on-line, searchable by crop, for all to use.  However, you might not
locate it without the following guidance from Barbara Herren.  For those
of you not familiar with the book, you will be amazed by the depth and
detail of coverage for even the most obscure crops.
===================================

It's at:
www.internationalpollinatorsinitiative.org.  

Once on this site, go to "Pollination Information Management System" link in the upper right.
From there, go to the second question,

# What is the current understanding of managing the pollination of a
particular crop?

You can then select a chapter, or enter a crop (common and scientific
names) in the search box to find a specific chapter.
==================================

We realise that there were some transcription errors in the conversion
over to a digital format, so my assistant, Dolores Boland, is reading the
entire book and making corrections to the pdfs, but this will take time to
complete. In the meantime, it is quite useable, even as is.

best,

Barbara
Herren, Barbara (AGPM) [
mailto:Barbara.Herren@...]

 

===============================

James H. Cane

USDA-ARS Bee Biology and Systematics Lab

Utah State University, Logan, UT 84322 USA

tel: 435-797-3879   FAX: 435-797-0461

email: Jim.Cane@... 

http://www.ars.usda.gov/npa/logan/beelab

http://www.biology.usu.edu/people/facultyinfo.asp?username=jcane

Gardening for Native Bees: http://extension.usu.edu/files/publications/factsheet/plants-pollinators09.pdf

 

"The obscure takes time to see,

but the obvious takes longer"
Edward R. Murrow

 


#1080 From: "Alex Surcica" <aps15@...>
Date: Thu May 13, 2010 2:55 pm
Subject: Height-adjustable Bee Bowl Traps
aps15@...
Send Email Send Email
 

Hello everybody,

 

I’m in the middle of a research project through which I monitor the bee populations on several farms located in the south-central PA. Last year, on several occasions I had high bowl casualties, mostly resulted from vehicle run over. In one instance I had more than 50% of the bee bowl traps run over—in part due to little communication and cooperation. One solution to mitigate this is to set the bowls in areas with less foot or vehicle traffic, which unfortunately are usually covered with vegetation. And while in the Spring the vegetation is relatively short and sparse, as the season progresses the bowls will be less visible for both bees and researchers. That said, after consulting with Sam, I came up with a relatively cheap and easy-to-install contraption that allows one to set bowls at whatever height level the vegetation might be. For the PowerPoint presentation, follow http://www.slideshare.net/sdroege/height-adjustable-bee-bowltraps. Let me know if you have questions or suggestions.

 

Thank you,

Alex

 

Slide1.JPG

Slide2.JPGSlide3.JPGSlide4.JPGSlide5.JPGSlide6.JPGSlide7.JPGSlide8.JPGSlide9.JPGSlide10.JPG

Slide11.JPG
     

Alex Surcică, Ag. Eng. (MS)

Pennsylvania State University – Coop. Ext.

181 Franklin Farm Lane, Chambersburg, Pennsylvania 17202

Phone: (717) 263-9226; Fax: (717) 263-9228; Email: Alex.Surcica@...

 

 


#1081 From: Azucena_Ponce@...
Date: Thu May 13, 2010 4:08 pm
Subject: Azucena 'Susi' Ponce is out of the office.
Azucena_Ponce@...
Send Email Send Email
 

I will be out of the office starting 05/13/2010 and will not return until 05/18/2010.


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