Ion-pairing reagents such as tetra-butyl-ammonium
chloride and octanesulfonic acid tend to adsorb to
reversed phase stationary phases like C-18, modifying
their chromatographic behavior. Once these reagents
have been used, it is recommended that the column be
dedicated to ion-pairing separations only.

As they are getting adsorption it needs more time for
column to be conditioned.


Khalid Ghaffar




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