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#2288 From: "Khristine Anderson" <resumes@...>
Date: Wed Feb 7, 2007 4:49 pm
Subject: [Job Advert] Senior Scientist/Group Leader - Analytical Development
michaelwilmarth
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Senior Scientist/Group Leader - Analytical Development

A great company in Tarrytown, NY is seeking a Senior Scientist/Group
Leader-Analytical Development.  Would you be interested in pursuing an
opportunity such as this?  If so, please review the details below. If not,
please consider referring a colleague.  Either way, thank you so much for
your time - have
a great day!

Responsibilities:

Assist the Director of Analytical Development with:

Primary Duties:
Development, optimization, and performance of characterization assays
designed to establish the primary, secondary and tertiary structure of
antibodies.
Technical writing of documents such as SOPS, investigation reports, assay
development and validation reports, and assay transfer protocols and summary
reports.
Interact with contract laboratories to establish service agreements, review
assay transfer and validation protocols, review and approve characterization
test results, and support investigation or trouble-shooting activities as
needed.  Development, optimization and performance of HPLC and spectroscopic
test methods to establish the conformational aspects and structure-function
relationships of antibodies.

Secondary Duties:
Review test results and laboratory notebooks prior to approval.  Develop
protocols for antibody characterization and comparability studies.
Preparation and participation in project management activities related to
product team meetings.  Provide backup support to Director, Analytical
Development.

Requirements:
Requires a Ph.D. in Analytical Chemistry or Protein Chemistry.
Experience in Biologicals analytical development laboratories that includes
5 or more years associated with:
Monoclonal antibodies and recombinant technology-derived protein product
development.
Development, transfer, and qualification of new analytical methods in
protein chemistry and antibody chemistry.
Demonstrated competency in the technical writing including SOPs, analytical
method development/antibody characterization reports, etc.
Performance of standard physico-chemical and conformational methods for the
characterization, and degradation pathway studies of clinical trial product
lots.
Hands on experience with the ion-exchange, hydrophobic and reversed phase
chromatographic test methods for antibodies.

Other requirements:
Knowledge of the GMPs as they relate to manufacturing and the Quality unit.
Attentive to details; strong communication and coaching skills;
team-oriented; comfortable with direction from Directors and peers.
Willing to travel if needed.  Identify and implement continuous improvement
of characterization assays.

Apply:
If you are interested in applying for one of these positions, please forward
your Word formatted resume, cover note and salary requirement to
resumes@.... Please include the specific position to
which you are applying in the subject line of your email. This will ensure
your resume is routed to the correct recruiter as quickly as possible.

We offer a referral 'bonus' program.  Here are the details:
http://www.workwondersstaffing.net/CandidateResources/Referrals.html

#2289 From: "zalghoul" <zahra_710@...>
Date: Fri Feb 9, 2007 3:25 pm
Subject: HPLC 1050
zalghoul
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Hello,
I'm working on an HPLC, Hewlett Packard 1050. I'm not being able to
detect any peak, even though I can see that the detector is reading an
absorbance signal on its screen. I'm a new user of the  instrument, so
I don't have enough experience to know how to start troubleshooting. I
would appreciate any suggestions.

#2290 From: "Khristine Anderson" <resumes@...>
Date: Thu Feb 15, 2007 9:11 pm
Subject: [Job Advert] Manager, Process Development - San Diego, CA
michaelwilmarth
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Manager, Process Development - San Diego, CA

A client of ours in San Diego, CA is seeking a Manager, Process Development.
They are a specialty biotechnology company focused on the development and
marketing of cell-based and tissue-engineered products.

Summary
This position is responsible for leading all of the activities related to
process development and improvements in the Company’s human cell culture
processes.  Candidate will design, develop and implement production
procedures that optimize manufacturing processes and meet regulatory
requirements.  Extensive time is spent preparing process development reports
and communicating to management, interacting with many different internal
departments, outside experts and consultants.

ESSENTIAL DUTIES AND RESPONSIBILITIES
The duties and responsibilities of this position include the following,
although other duties may be assigned.
Evaluate improvements for current manufacturing processes and manufacturing
procedures for new products;
Make recommendations for which process improvements should be pursued,
including those involved in scaling up current manufacturing processes, and
for which manufacturing procedures should be used for new products;
Direct the development and validation of process improvements and
manufacturing procedures for new products, which includes growing human
cells aseptically and preserving and handling the final sterile cell
product;
Oversee the training of production personnel in these process improvements
and manufacturing procedures for new products; and
Lead technical troubleshooting for problems that develop in our current
manufacturing procedures.
Supervisory Responsibilities
This position manages one or two subordinates, who perform laboratory
procedures involved in the development and improvement of manufacturing
processes.  The responsibilities include planning, assigning and directing
work; evaluating performance; rewarding and disciplining employees;
addressing complaints and resolving problems.  The responsibilities may
include interviewing and hiring employees.

Qualifications
To perform this job successfully, an individual must be able to perform each
essential duty satisfactorily.  The requirements listed below are
representative of the knowledge, skill and/or ability required.  Reasonable
accommodations may be made to enable individuals with disabilities to
perform the essential functions.
Thorough understanding of Good Manufacturing Practices (GMP) and Quality
System Regulations (QSR).  Good working knowledge of clean room practices
and aseptic processing.
Strong training skills: Able to train employees in manufacturing procedures.
Strong communication skills (written & oral): For preparing technical
reports, interacting with peers and presenting reports to upper management.
Leadership skills: Delegate, develop and motivate personnel.
Analytical skills: Able to analyze complex data and make recommendations for
actions.
Technical Skills: Operation of clean rooms; understanding Quality System
Regulations & Good Manufacturing Practices and safely in the workplace.
Planning/Organizational Skills:  Able to: take an objective or goal,
determine all of the tasks and milestones required to meet the goal and
organize the tasks with achievable timelines and within acceptable
standards.  A detail oriented person that can conceive, research, target and
control reductions in cost and product lead times on both existing and new
products.  A problem solver with the ability to manage and motivate
employees.
Implementation Skills: Able to develop broad goals and objectives and
translate them into achievable milestones and action plans.  Possess the
capability to follow through with assignments and capable of interacting
with others to do the same.  Able to administer and facilitate change.
Strong background and skills in process development and improvement.

Computer Skills: Including working knowledge and experience with Excel and
MRP a plus.
People Management Skills: Good organizational, problem solving,
communication and negotiating capabilities.  Able to adapt and administer
change.

Education and/or Experience
Minimum of a BS degree and a minimum of ten years experience in
biotechnology-related (cGMP) manufacturing process development and
improvement, which must include extensive experience in growing mammal
cells, preferably human fibroblast cells, and process validation.  A Ph.D.
in process engineering and development a strong plus.
A strong background in cell biology is critical.
Minimum of 3 years managing employees.
Demonstrated experience interacting with other departments and functions.
Experience with regulatory agency audits and knowledge of GMP compliance.

Interested candidates should submit to resumes@...:

1. Brief cover letter/note
2. Resume in Word or Txt format (not PDF please)
3. Salary requirement.  If 'open' or 'negotiable', please provide specific
details concerning your salary history.
4. Work authorization (i.e. citizen, h1b, etc)

#2291 From: "rindkursataktas" <rindkursataktas@...>
Date: Sun Feb 18, 2007 3:17 pm
Subject: mycotoxin analysis
rindkursataktas
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Dear members of the list,

i have been appointed to a new laboratory and i am expected to learn
how to detect mycotoxins in food and feed materials. firstly i want to
be aware of new trainig programs about mycotoxin analysis with
biosensors and of course new techniques to detect mycotoxins.if any of
you could help me to reach my goals i would be very glad.
thanks in advance

Rind Kursat aktas
ministry of Agric.
and rural affairs of Turkiye
Ank. provincial Control Lab.

#2292 From: "Khristine Anderson" <resumes@...>
Date: Tue Feb 13, 2007 4:23 pm
Subject: [Job Advert] Senior Analytical Scientist - Torrance, CA
michaelwilmarth
Offline Offline
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Senior Analytical Scientist - Torrance, CA

I am assisting to recruit a Sr Analytical Scientist for a great company in
Torrance, CA.  Would you be interested in an opportunity such as this? If
so, the details are below for your review. If not, please consider referring
a colleague.  Either way, thank you for your time and have a great day!

The Sr Analytical Scientist reports to the Senior Director, Quality Control.

JOB FUNCTION:

The Senior Analytical Scientist fulfills a critical role in active
pharmaceutical ingredient development and manufacturing operations.  This
individual must develop and implement analytical test methods, plan and
execute method validations studies, write validation protocols, reports, and
other technical quality documents.  This individual conducts or contributes
to investigations related to analytical methods.  This individual must also
coordinate, execute and oversee impurity profiling and peptide
characterization projects.  Additionally, this individual contributes to
advances in analytical capabilities for the Quality Control (QC) department
and to the development of all QC staff members.

ESSENTIAL FUNCTIONS:

Execute method development and validation activities, which include:
Development and optimization of new and existing analytical methods for
in-process control and QC release/stability testing
Troubleshooting and investigation of poorly performing analytical methods
Development of methods for characterization of peptides, related substances,
and degradation products
Validation of in-process control and release/stability test methods in
accordance with appropriate regulatory guidelines, including forced
degradation and photostability studies
Conduct or contribute to out of specification (OOS), validation failure, and
other QC-initiated investigations related to analytical methods
Coordinate, execute and oversee impurity profiling and peptide
characterization projects for the establishment of specifications and in
support of regulatory CMC filings
Expand analytical capabilities of the QC department
Evaluate, recommend, and implement new analytical technologies and
instrumentation for peptide analysis and characterization
Provide training and guidance to QC staff members aimed at developing
technical capabilities

ADDITIONAL RESPONSIBILITIES:

1.    Participate in GMP customer audits and regulatory agency inspections
2.    Stay abreast of new developments in technologies for analysis and
characterization of peptides
3.    Support QC release and stability testing on an as needed basis
4.    Contribute to improvement in laboratory operations to increase
efficiency and GMP compliance

EDUCATIONAL REQUIREMENTS:

PhD in Chemistry or Analytical Chemistry

EXPERIENCE & QUALIFICATION:

1.    5 or more years experience in peptide analytical chemistry
2.    Broad knowledge of modern analytical chemistry
3.    Hands-on experience with HPLC, GC, MS, LC-MS

SKILLS:

1.    Thorough understanding of peptides and their chemistry
2.    Self-motivated with the ability to work independently, set priorities,
and follow through on commitments
3.    Team player with the ability to interact well with co-workers
4.    Analytical thinker with the creativity to solve technical and
compliance problems
5.    Enthusiasm and dedication
6.    Detail oriented
7.    Excellent English skills, both written and oral
8.    Excellent communication and presentation skills.
9.    Technical writing skills
10.  Safe and proficient laboratory practice.
11.  Extensive computer skills

If interested, please respond with resume, salary requirement, relocation
considerations and work authorization.

We do have a referral 'bonus' program.  Here are the details:
http://www.workwondersstaffing.net/CandidateResources/Referrals.html

#2293 From: rind <rindkursataktas@...>
Date: Thu Feb 15, 2007 5:37 pm
Subject: mycotoxin analysis
rindkursataktas
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dear members of the list,
   i am looking for a training program for mycotoxin analysis by using
biosensors. would someone lead me an addres to solve my problem?

   Rind


---------------------------------
Now that's room service! Choose from over 150,000 hotels
in 45,000 destinations on Yahoo! Travel to find your fit.

[Non-text portions of this message have been removed]

#2294 From: "chang" <chang@...>
Date: Mon Feb 12, 2007 2:12 pm
Subject: To the Isoflavone guys....
changuinho2000
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I'm running some HPLC soybeans extracts with ETOH 70% with acetic acid
0,1%, but I got no Genistein peak, although I got a peak at gradient run at
37 minutes but with 294 UV absortions, instead of 260 nm......
     Could it be degradated or to be in malonyl form ?
     Thanks for any help....

#2295 From: "the shifflet's" <shiffty@...>
Date: Sat Feb 24, 2007 12:44 pm
Subject: Re: [SPAM] HPLC 1050
shiffty@...
Send Email Send Email
 
Hi,

   Is the system conected to an integrator or to a chromatography station?

In either case I would first check to see if the cables are connected tightly.

   Do you see any noise on the data system or is it just a flat line?

  thanks,

mike


   ----- Original Message -----
   From: zalghoul
   To: chrom-L@yahoogroups.com
   Sent: Friday, February 09, 2007 10:25 AM
   Subject: [SPAM] [chrom-L] HPLC 1050


   Hello,
   I'm working on an HPLC, Hewlett Packard 1050. I'm not being able to
   detect any peak, even though I can see that the detector is reading an
   absorbance signal on its screen. I'm a new user of the instrument, so
   I don't have enough experience to know how to start troubleshooting. I
   would appreciate any suggestions.





[Non-text portions of this message have been removed]

#2296 From: Tony_Herlt <herlt@...>
Date: Sun Feb 25, 2007 10:17 pm
Subject: article wanted
herlt@...
Send Email Send Email
 
26th February 2006

Dear colleagues,

I am trying to obtain the following article:

Title:   Analysis of peptides at low levels using a
chromatography system optimized for narrow-bore columns.

Authors: Burgoyne, Robert; Stacey, Catherine; Young, Patricia;
           Astephen, Nancy; Merion, Michael.    Waters Chromatogr.
Div.,  Millipore Corp.,  Milford,  MA,  USA.

Source:  Tech. Protein Chem.  (1989), 399-413.  Publisher:
       Academic,  San Diego, Calif

If any of you have this reference I would be grateful for a copy.

Thank you in advance,

Kind regards,


Tony Herlt

Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:  +61 (0)2 6125 9765
mobile: 0404 823 159
fax:  +61 (0)2 6125 0750
e-mail: herlt@...
----------------------

#2297 From: "Gianfranco Bruno" <gianfranco.bruno@...>
Date: Mon Feb 26, 2007 5:12 pm
Subject: 25 OH Vitamine D3
gbruno_2000
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I need the components of a simple method to determine Vitamine 25 OH
D3 in HPLC.The method is as follow:

Sample preparation

Mix briefly 500 µl serum/plasma with 50 µl Internal Standard in a
light protected reaction vial.
Add 500 µl Precipitation Reagent, vortex 20 s.
Cool down 10 min at +4 °C.
Centrifuge 5 min at 13 000 rpm.
Apply entire supernatant to sample clean up column, centrifuge 1 min
at 1500 rpm, discard effluent.
Draw 2 x 1 ml Wash Buffer 1 through the column by centrifugation (1
min at 1500 rpm), discard effluent.
Draw 75 µl Wash Buffer 2 through column by centrifugation (1 min at
1500 rpm), discard effluent.
Elute with 200 µl Elution Buffer into glass vials (1 min at 1500 rpm),
dilute with 20 µl dist. water.
Inject 50 µl eluate into the HPLC system.

What are the names of these components according to you?

Thanks

Dr Gianfranco Bruno
Italy

#2298 From: "Gianfranco Bruno" <gianfranco.bruno@...>
Date: Sun Mar 25, 2007 8:45 pm
Subject: Extraction of plasma catecholamines
gbruno_2000
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We use a method to determine plasma catecholamine in HPLC.Prior the
plasma is precipitated with a reactive A.Then exctrated with a resin
mixed to dietiletilammonium.
When I have used for raective A a perchloric acid I have seen that I
have not extraction of catecholamines but I have only Internal
standard put prior!

Any suggestions?

GB

#2299 From: "j_sadikali" <j_sadikali@...>
Date: Thu Mar 29, 2007 10:04 am
Subject: Chef test
j_sadikali
Offline Offline
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Dear Friends

Could you anyone suggest us for doing Chef test and swap test in
water,meat and fish.

Charm sciences inc is making such instrument.

If you know any equivalent inst. please let me know the details.

Thanks in advance.

Regards
Jawahar Sadikali.

#2300 From: "nfitzgerald" <neil.fitzgerald@...>
Date: Wed May 2, 2007 8:08 pm
Subject: GC software advice
nfitzgerald
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We have a HP5890 GC-FID and a Varian 3400 GC-ECD.  Both currently have
integrators.  I'd like to replace both integrators with one computer.
  We never use both instruments at the same time and I'm happy to
switch the computer from one to the other.  Does anyone have any
suggestions on good, cheap software that would work for both
instruments?  I just need something simple (display peaks and
integrate).  We have an old ADC for the Varian that I think still
works but I guess would need something similar for the HP.

Cheers.

#2301 From: Tony_Herlt <herlt@...>
Date: Wed May 2, 2007 11:29 pm
Subject: Re: GC software advice
herlt@...
Send Email Send Email
 
3rd May 2007

There is chromatography data system software called Delta 5.5 that would
probably work well with your GC instruments and is competitively priced.

The URL is: http://www.dataworx.com.au/www/index.htm .

HTH,

Tony

nfitzgerald wrote:
> We have a HP5890 GC-FID and a Varian 3400 GC-ECD.  Both currently have
> integrators.  I'd like to replace both integrators with one computer.
>  We never use both instruments at the same time and I'm happy to
> switch the computer from one to the other.  Does anyone have any
> suggestions on good, cheap software that would work for both
> instruments?  I just need something simple (display peaks and
> integrate).  We have an old ADC for the Varian that I think still
> works but I guess would need something similar for the HP.
>
> Cheers.
>
>

--
----------------------
Tony Herlt
Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:  +61 (0)2 6125 9765
mobile: 0404 823 159
fax:  +61 (0)2 6125 0750
e-mail: herlt@...
----------------------

#2302 From: "creakinator" <ckeller@...>
Date: Wed Jul 18, 2007 8:38 pm
Subject: GC/MS autotune questions
creakinator
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Problem:  We are consistently seeing peak(s) around 14-18 amu over
40 abundance in the autotune spectra on an Agilent 5973 MSD
connected to an Agilent 6890 GC.  We had a technician come out and
the only solution to the problem is to do a startup.m method that
starts at 60C, Initial Time =0, Ramp of 30 to 250 for a final time
of 10 mins, run time is 16.33 mins.  The Front Inlet parameters are
Split mode at 250C, pressure at 5.30 psi, split ratio – 23.5:1,
split flow – 35.7 ml/min, total flow 39.3 ml/min.  The tech checked
for leaks through out the entire system

The general.m method that we use for the analysis is a bit different
being in a splitless mode, start temp of 60C, Initial time 2 mins,
rate 30C, 300C for 5 mins..  Front Inlet splitless 250C, 5.30
pressure, purge flow 35.7, total flow 39.3.

We created a new method called gensplit.m where the only thing we
changed in the method was the split mode and the peaks were greatly
reduced.

The questions, other than the problem stated above, are:
1. Why are we seeing these peaks?  What could they be – water,
nitrogen, oxygen??
2. Is it really a problem to run an autotune using a different
method than what you are running the samples on?  I don't think so
as the autotune is checking the MS of the instrument.  When I
compared the startup.m to the general.m the only differences are in
the GC parameters and none in the MS parameters.
3. Any advantages or disadvantages to running split or
splitless?

Some people think that is moisture built up in the GC system and
when we do the startup.m, the moisture is burned off as the peaks
are lower if the autotune is done immediately after the startup.m
methods ends.  But could it be another contaminant?

The analysis on this GC/MS is for controlled substances, qualitative
analysis only.

Thank you for your help.

Christy Keller
San Bernardino County Sheriff's Department
Scientific Investigation Division

#2303 From: "Gianfranco Bruno" <gianfranco.bruno6@...>
Date: Thu Sep 20, 2007 3:59 pm
Subject: Condionining and ion pair
gbruno_2000
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Why a C18 Column with a mobile phase with ion pairing conditions on more time
than a C18 with a mobile phase without ion pairing?

thank you

Gianfranco

[Non-text portions of this message have been removed]

#2304 From: "Gianfranco Bruno" <gianfranco.bruno6@...>
Date: Mon Oct 1, 2007 5:11 pm
Subject: VMA and Ion pairing
gbruno_2000
Offline Offline
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Please I need this article or similar:


Thank you

Gianfranco
AU: N. Lammers, J. Zeeman, G. J. De Jong
TI: Effect of some parameters on the liquid chromatographic separation of
biogenic amines and their metabolites
SO: Journal of High Resolution Chromatography
VL: 4
NO: 9
PG: 444-447
YR: 1981
CP: Copyright © 1981 Dr. Alfred Hüthing Verlag GmbH
ON: 1521-4168
PN: 0935-6304
AD: Research Laboratories, Duphar, P.O. Box 2, 1380 AA Weesp, The Netherlands
DOI: 10.1002/jhrc.1240040904
US: http://dx.doi.org/10.1002/jhrc.1240040904
AB: The liquid chromatographic separation of some biogenic amines and their
metabolites with a reversed-phase ion-pair system has been optimized. The
effects of the nature of the stationary phase, the column temperature, the pH,
and the ion-pair reagent concentration of the mobile phase have been
investigated. The most striking result is the influence of the column
temperature on selectivity.

!

[Non-text portions of this message have been removed]

#2305 From: Khalid ghaffar <khalidghafar@...>
Date: Tue Oct 2, 2007 7:13 am
Subject: Re:Condionining and ion pair
khalidghafar
Offline Offline
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Ion-pairing reagents such as tetra-butyl-ammonium
chloride and octanesulfonic acid tend to adsorb to
reversed phase stationary phases like C-18, modifying
their chromatographic behavior. Once these reagents
have been used, it is recommended that the column be
dedicated to ion-pairing separations only.

As they are getting adsorption it needs more time for
column to be conditioned.


Khalid Ghaffar



      
________________________________________________________________________________\
____
Don't let your dream ride pass you by. Make it a reality with Yahoo! Autos.
http://autos.yahoo.com/index.html

#2306 From: Guilherme Vannucchi Portari <gv_portari@...>
Date: Thu Oct 18, 2007 6:06 pm
Subject: Articles
gv_portari
Offline Offline
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Dear Colleagues,
Can someone send me this article,

Lechner M, Rieder J. Mass spectrometric profiling of low-molecular-weight
volatile
compounds--diagnostic potential and latest applications. Curr Med Chem.
2007;14(9):987-95.

or othes that discuss about problems in injecting air into the GC / MS.
Thanks.

--------------------------------------------------------------
Guilherme Vannucchi Portari
Farmacêutico-Bioquímico
Técnico da Disciplina de Bromatologia do Curso de
Nutrição e Metabolismo - USP Ribeirão Preto
F: 55 (16) 3602-4629
------------------------------------------------------------------------------
Technician of Food Science Laboratory
Nutrition and Metabolism Faculty
University of Sao Paulo - Brazil
---------------------------------------------------------------
E-mail: gv_portari@...
---------------------------------------------------------------


       Abra sua conta no Yahoo! Mail, o único sem limite de espaço para
armazenamento!
http://br.mail.yahoo.com/

[Non-text portions of this message have been removed]

#2307 From: "noziraf" <y.farizon@...>
Date: Mon Oct 22, 2007 9:37 am
Subject: Lactic acid analyse
noziraf
Offline Offline
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Did someone have experience for quantifying low concentration of
lactic acid in rumen juice (10 µg/ml)with HPLC/UV or GC/FID system ?
Thanks.

#2308 From: Tony_Herlt <herlt@...>
Date: Mon Dec 3, 2007 11:47 pm
Subject: basic alumina HPLC column
herlt@...
Send Email Send Email
 
4th December 2007

Dear colleagues,

Would any of you know of a commercial source for an HPLC column packed
with basic alumina (pH >= 9), particle size 10 micron or below.  I would
be interested in 4.6mm and 20mm internal diameters.

Thank you for your time.

Kind regards,

Tony

----------------------
Tony Herlt
Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:  +61 (0)2 6125 8339
mobile: 0404 823 159
fax:  +61 (0)2 6125 0750
e-mail: herlt@...
----------------------

#2309 From: Guilherme Vannucchi Portari <gv_portari@...>
Date: Tue Jan 15, 2008 1:29 am
Subject: article request
gv_portari
Offline Offline
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Dear colleagues,
can somebody send me the article below?


Ng HT, Chen CY, Hsieh HM, Leung WY, Ou MC, Tang WL. Amniotic fluid
lecithin/sphingomyelin ratio in high risk pregnancy.Proc Natl Sci Counc Repub
China B. 1988 Oct;12(4):247-51.

Thanks!


--------------------------------------------------------------
Guilherme Vannucchi Portari
------------------------------------------------------------------------------
Food Science Laboratory
Nutrition and Metabolism Faculty
University of Sao Paulo - Brazil
---------------------------------------------------------------
E-mail: gv_portari@...
---------------------------------------------------------------


       Abra sua conta no Yahoo! Mail, o único sem limite de espaço para
armazenamento!
http://br.mail.yahoo.com/

[Non-text portions of this message have been removed]

#2310 From: "nfitzgerald" <neil.fitzgerald@...>
Date: Mon Mar 3, 2008 4:36 pm
Subject: PCB ECD problem
nfitzgerald
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Hi all,
I'm hoping someone could help me.  We have started some experiments
with PCBs.  We extracted control and PCB spiked sediment samples by
soxhlet (hexane/ acetone).  We can see the PCBs in the spike by GCMS
but not ECD.  The ECD gave peaks for an SRM and for the test mixture
but for these samples (Hudson river sediment). I have to turn up the
ECD attenuation and get just a couple of really tiny peaks.  I find it
difficult to believe the ECD is less sensitive than the MS.  We tried
adding some small pieces of clean copper to see it was a sulfur
problem but no improvement.

Any ideas or suggestions?

Thanks

#2311 From: Guilherme Vannucchi Portari <gv_portari@...>
Date: Mon Mar 3, 2008 7:33 pm
Subject: off topic
gv_portari
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Dear Colleagues,
Excuse me to use the forum for this help. I'm needing an old article about
glutathione dosage. If somebody can send me I will thank a lot.

Jozef Sedlak and Raymond H. Lindsay. Estimation of total, protein-bound, and
nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical
Biochemistry. Volume 25, 1968, Pages 192-205.

--------------------------------------------------------------
Guilherme Vannucchi Portari
------------------------------------------------------------------------------
Technician of Food Science Laboratory
Nutrition and Metabolism Faculty
University of Sao Paulo - Brazil
---------------------------------------------------------------
E-mail: gv_portari@...
---------------------------------------------------------------


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#2312 From: Tony_Herlt <herlt@...>
Date: Tue Mar 11, 2008 6:40 am
Subject: CrestPak C18 HPLC columns
herlt@...
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11th March 2008

Dear colleagues,

Would any of you have information on uS Crestpak C18-S HPLC columns (u
represents the Greek letter, mu), specifically, manufacturer, carbon
load, monomeric/polymeric functionality etc.

Thank you for your time,

Kind regards,

Tony Herlt.
----------------------
Tony Herlt
Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:  +61 (0)2 6125 8339
mobile: 0404 823 159
fax:  +61 (0)2 6125 0750
e-mail: herlt@...
----------------------

#2313 From: "noziraf" <y.farizon@...>
Date: Mon Mar 31, 2008 2:45 pm
Subject: peroxydation of linoleic acid
noziraf
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Anyone have simple method for preparation of hydroperoxy linoleic acid
(13-HPOD and 9-HPOD) and hydroxy linoleic acid (13-HOD and 9-HOD) with
linoleic acid ?
Anyone have simple method for analysing these coumpounds in RPLC/UV or
adsorption LC/UV with vegetable oil samples?

#2314 From: "nfitzgerald" <neil.fitzgerald@...>
Date: Thu Jul 24, 2008 6:49 pm
Subject: GCMS noise
nfitzgerald
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Hi,
I'm having some noise issues that I'm hoping someone can help me with.
  I have a HP5890 GC and HP5970 MS.  I recently fixed the injector
heater and replaced the column.  It seemed to work OK for a while but
I have a high background (about 1E6).  I tried reinstalling the column
a couple of times but now can't get any peaks at for a test mixture
(0.03% C14, C15, C16)not even for the solvent, just lots of noisy
spikes.  The water level is a little high about 40%.  I'm running out
of patience with it.  Any advice?

Cheers,

#2315 From: Tony Herlt <herlt@...>
Date: Wed Aug 27, 2008 12:44 pm
Subject: HPLC of 1,2-dialkylcyclohexanes
herlt@...
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27th August 2008

Dear colleagues,

We are trying to separate the cis/trans isomers of
1,2-dialkylcyclohexanes by HPLC, the aim ultimately being to obtain up
to 100 milligrams of each isomer.  Slight separation is achieved on a
Cronusil Triple-E C18 column with acetonitrile (see attached pdf) but
this is insufficient to consider a preparative separation.  Preparative
GC might work but we no longer have the facility here.  Any suggestions
on alternative HPLC columns are very welcome.  Thank you for your time.

Kind regards,

Tony Herlt.

--
----------------------
Tony Herlt
Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:     +61 (0)2 6125 8339
mobile: 0404 823 159
fax:     +61 (0)2 6125 0750
e-mail: herlt@...
----------------------



[Non-text portions of this message have been removed]

#2316 From: "nfitzgerald" <neil.fitzgerald@...>
Date: Wed Sep 10, 2008 2:44 pm
Subject: GCMS noise follow up
nfitzgerald
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Thanks for everyone's help with my GCMS noise problem.  I thought it
might be good to post a follow up.  After replacing the column,
injector heater, liner, and injector seal, it seems that i managed to
put a crack in the column.  Therefore, very little gas was getting to
the detector hence the noisy signal.

Again, thanks to all for your advice.

#2317 From: Tony_Herlt <herlt@...>
Date: Thu Nov 12, 2009 11:15 pm
Subject: C14 calibration cell for Beckman 171 Radioisotope Detector
herlt@...
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Dear colleagues,

A colleague has obtained a second-hand Beckman 171 radioisotope detector
   and needs to calibrate it for C14 but does not have the calibration
cell.

Would any of you know where we might be able to obtain or borrow one?

Thank you in advance for any help,

Tony Herlt.
----------------------
Tony Herlt
Research School of Chemistry
Building 35, Australian National University campus
Science Road, Acton
Canberra, ACT 2601
Australia

phone:  +61 (0)2 6125 8339
mobile: 0404 823 159
fax:  +61 (0)2 6125 0750
e-mail: herlt@...
----------------------

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