What's your optimized conditions for organophosphorous pesticide with megabore column (JW DB5 0.53 mm i.D and 15 m length )and this specific instrument CPG...
hi guys I'm a french student in bachelor of chemical engineering and I carry out some analysis on amino acids I have to identify two amino acids, glycine and...
Hi, I hope someone can help me with this problem. I've been trying to set up a method to analyse PAH's on HPLC. Now that ain't the problem really, I've got a...
Dear members of this list, I need information in order to get Internet address to buy scientific book apart from AMAZON. I need an used book which was...
DRDN
tequila2@...
Jul 9, 2003 9:11 am
1635
Hi, I have two questions: 1. Are you trying to find low levels of PCBs? 2. what are your chromatographic conditions. mike ... From: jevdende...
The Shifflet's
shiffty@...
Jul 10, 2003 9:56 am
1636
Hi, Could you elaborate on the HPLC system. I am curious about the problems with a blank injection of solvents. Thanks, mike ... From: nfitzgerald...
The Shifflet's
shiffty@...
Jul 10, 2003 10:02 am
1637
Dear all, I am new to HPLC. I did the worst thing with the brand new HPLC column (Bondapak C18) by forgot to check the amount of the mobile phase and it all...
Dear Kimmikar, Disconnect the column from the detector end and raise the column so that the disconnected end is higher than the injector side. Flow 100%...
Tony Herlt
herlt@...
Jul 11, 2003 11:39 pm
1639
Dear Kimmikar A good way to get rid of the air bubbles is to wash your column with 100% organic mobile phase. If there is a inorganic salt in the mobile phase...
Hi everyone on the Chrom-L group! My name is Bob Albrecht. I am formerly of LC Resources and Biotage, Inc. My specialty is software and instrumentation for...
Dear all, I am doing a quantitative analysis of Vitamin E and Vitamin E acetate. The retention time of them in ACN:Water (96:4) are 13 and 16 minutes. I would...
5 Suggestions: 1. Add more acetonitrile or switch to a different solvent. 2. Buy a shorter or less retentive column 3. Raise the flow rate 4. Raise the column...
Dear colleagues, we are looking into replacing our current TEA (Thermal Energy Analyzer) detector. I am aware that there are very limited sources for this type...
Diego Zurbriggen
Diego_Zurbriggen@...
Jul 25, 2003 6:26 pm
1644
We have noticed something curious about our DAD and don't know whether this is normal or we are programming it incorrectly. We choose the baseline, apex and...
Dear chrom-L members, I am searching for papers and sources of informaation on modification to chromatography theory to account for permanent adsorption of...
Hi everybody I would like to know if anybody knows how to measure the area of the peaks from the chromatograms and mass spectra. Thanks for any replies, Lilia...
Dear All We have some money to purchase some ion chromatography kit (both anion and cation with autosamplers) and have been in contact with Dionex and Metrohm....
Dear ALL I am planning to analyse some endogenous compounds in human plasma, would anybody know how to prepare plasma blank (without endogenous compounds) ? ...
We have recently been donated an perkin elmer liquid chromatograph with this modules: 1. Pe nelson 900 series interface 2. Pe nelson 600 series link 3. uv /vis...
At 10:08 28/07/03 +0100, you wrote: Hi Jonathan I have done ion Chromatography using Dionex Instrument and found to be really good both for anion and cation....
Emmanuel Samuel
emmanuel.samuel@...
Aug 1, 2003 12:56 pm
1651
Dear all Can anyone tell me about a "standardized procedure for the extraction and quantifictaion of soyasaponins in soybeans and foods by using High...
My institute plans to buy a Maldi-Tof mass spectrometer, to be used in research work involving proteomics and drug development. Can any member of the group...
Dear All, Can someone advise me on how to improve yields when purifying natural products via HPLC/UV? Any tips or even a listing of the factors involved would...
If you are trying to purify a single component from a natural product, then resolution is everything. Extending the gradient time (shallower gradient) until...
Dear all Can anyone tell me "if a mobile phase of 0.1% TFA in water and 0.1% TFA in Acetonitrile can harm a C-18 ODS column?"I require these solvents to run...
Dear all: The retention time of a standard is changeble when i use HPLC-FLD.For example:the same standard's retention time is not stable(the first injection is...
Normal use of 0.1 % TFA mobile phases should not harm your HPLC systems. I would not store the column or the HPLC system with the 0.1 % TFA. Prior to storage...
The Shifflet's
shiffty@...
Aug 12, 2003 2:34 am
1658
Hi, How long are you conditioning your column. It would seem that the column is not completely conditioned prior to your first injection. Depending on your...
The Shifflet's
shiffty@...
Aug 12, 2003 2:55 am
1659
Are you injecting some blanks before your standards? As The Shifflet's said, it seems that your column is not well conditioned, maybe the solvent was finished ...
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