We have recently been donated an perkin elmer liquid chromatograph with this modules: 1. Pe nelson 900 series interface 2. Pe nelson 600 series link 3. uv /vis...
At 10:08 28/07/03 +0100, you wrote: Hi Jonathan I have done ion Chromatography using Dionex Instrument and found to be really good both for anion and cation....
Emmanuel Samuel
emmanuel.samuel@...
Aug 1, 2003 12:56 pm
1651
Dear all Can anyone tell me about a "standardized procedure for the extraction and quantifictaion of soyasaponins in soybeans and foods by using High...
My institute plans to buy a Maldi-Tof mass spectrometer, to be used in research work involving proteomics and drug development. Can any member of the group...
Dear All, Can someone advise me on how to improve yields when purifying natural products via HPLC/UV? Any tips or even a listing of the factors involved would...
If you are trying to purify a single component from a natural product, then resolution is everything. Extending the gradient time (shallower gradient) until...
Dear all Can anyone tell me "if a mobile phase of 0.1% TFA in water and 0.1% TFA in Acetonitrile can harm a C-18 ODS column?"I require these solvents to run...
Dear all: The retention time of a standard is changeble when i use HPLC-FLD.For example:the same standard's retention time is not stable(the first injection is...
Normal use of 0.1 % TFA mobile phases should not harm your HPLC systems. I would not store the column or the HPLC system with the 0.1 % TFA. Prior to storage...
The Shifflet's
shiffty@...
Aug 12, 2003 2:34 am
1658
Hi, How long are you conditioning your column. It would seem that the column is not completely conditioned prior to your first injection. Depending on your...
The Shifflet's
shiffty@...
Aug 12, 2003 2:55 am
1659
Are you injecting some blanks before your standards? As The Shifflet's said, it seems that your column is not well conditioned, maybe the solvent was finished ...
Valentina Lonzarich
valentina13@...
Aug 13, 2003 8:11 am
1660
hello everibody: I am looking for chromatographic methods in order to analize substances used as chemical weapon, can you help me? ...
janneth ruiz
jannetheruiza@...
Aug 13, 2003 10:52 am
1661
I'm a biotech recruiter specializing in chromatography. Please feel free to send your resume, forward my information for anyone looking for a new position....
We were conversing moments ago, & as I cautioned, my battery expired in mid word. I tried calling back on another 'phone, but your line busy. I'll be home in...
martinrisk@...
Aug 15, 2003 9:16 pm
1663
Hi, I'm student of chemistry in Slovakia, and I need some articles from Journal of Separation Science (which is here absolutely unavialable). Does anyone has...
Dear All, Our customer needs Headspace sampler for GC Chrompack CP 9001. Could you please anybody, inform what brand is compatible with this GC system. Thanks...
Hello for all I am looking for the method of analysis for Thrombokinase activity, by clotting time. Is it possible to someone send this method for me (FAX or...
Hi folks. I have a general question about separation techniques for small molecules. I want to separate two small molecules that differ only by 50 Da. The...
27th August 2003 Dear colleagues, Would any of you know how to configure an HP1090 instrument with a manual injector when running ChemStation 7.2. The...
Tony Herlt
herlt@...
Aug 27, 2003 10:10 am
1668
i would say big difference of 50Da based on what you said on HPLC. CE also will probably work. why don't you just try a gradient run on reverse phase HPLC c18...
Ziqiang Wang
ziqiang@...
Aug 27, 2003 1:08 pm
1669
This all depends on their structures. It is easy enough to separate two compounds that differ by a propyl group, 42 Da, by reversed phase. However, since you...
Hi, if there are no differences in polarity and charge, neither CZE, nor RPLC will help you. If there is a difference in size only, GPC or FFF (field flow...
Milan Minárik
m_minarik@...
Aug 29, 2003 3:36 pm
1671
i would think CZE will at least show separation since he said the polarity, etc. are similar but the MW is different, is it true that CZE is based on m/z...
Ziqiang Wang
ziqiang@...
Aug 30, 2003 3:19 am
1672
What are the molecules? Hydrophobicity is very important in HPLC but so is molecular shape and position of moities on a molecule.
Mike
-----Original...
The Shifflet's
shiffty@...
Aug 31, 2003 12:20 pm
1673
Did someone just recently win the nobel prize for this? Could you give me his name? Also, I'm thinking of doing a talk on this and am wondering if there are ...
... For all your Nobel needs, try www.nobel.se Perhaps you are thinking of John Fenn and Koichi Tanaka who won part of the prize (it's shared among 3 people)...
We have a a Varian Saturn 2000 GCMS. At the moment we are experiencing major problems. We are currently changing filaments every 4 weeks or so, sometimes after...
A few years ago I set up another good chromatography resource online. It is located at http://ChromForum.com If your questions go unanswered here, you might...
dear all: These are my chromatogram picture.What's the matter on earth? The first one is the mixed standard ; The second one is the first injection of one...
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