Dear Cheng,



I am quite new at running DChipSNP, and have a basic question; when I am trying
to filter genes in preparation of gene clustering analysis (for now on the NSP
250k Affy chip), I am getting the message “0 of 262264 probe sets satisfied the
filtering criteria: No probe set filtered. You may uncheck 'Tools/Array list
file/Use standardize separator' and retry
Variation across samples: 0.50 < Standard deviation / Mean < 1.50
P call % in the array used >= 20%
The expression level >= 20.00 in >= 20% samples”

Even though I changed the criteria in all sorts of ways, I still got no list of
filtered genes. Could you please help clarify what I am missing?


Note: I chose the option "using all genes, and then the "Mapping250K_Nsp snp
info.txt" for the gene list, and am not clear whether this might have triggered
the problem.

Many thanks.
Corina



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Corina J. Shtir
Research Associate
University of Southern California
Doheny Eye Institute
1450 San Pablo St.
DEI - 4803
Los Angeles, CA 90033
Office: 323.442.6486
Cellular: 714.337.1052
Email: stir@...