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Messages 2544 - 2589 of 2747   Oldest  |  < Older  |  Newer >  |  Newest
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2544
Hello,I tried to use DchipSNP on 50k Xba 240 chip, but the software doesn't work without the cdf file, how can I get it?Thanks.Christophe PouletLaboratoire de...
Christophe Poulet
christophe_p...
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Feb 2, 2007
9:20 am
2545
Hello Christophe, Try http://www.affymetrix.com/support/technical/libraryfilesmain.affx HTH, Xavi. ________________________________ De: dchip@yahoogroups.com...
Sole Acha, Xavi
xsole2002
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Feb 2, 2007
10:22 am
2551
General array/clustering question- can I generate a heat map to compare the expression between the same cell type but different subjects, without comparing to...
M Braunstein
braunstein_m
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Feb 5, 2007
4:37 am
2552
hi cheng, in "displaying range of standardized values" i wanted to confirm that the range "-3 to +3" refers to negative values= increasing negative log ratios ...
aeonlanack
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Feb 5, 2007
6:09 pm
2553
is it log2 versus mean?...
aeonlanack
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Feb 5, 2007
6:09 pm
2554
Aeonlanack, They represent standardized values of non-logged raw expression values for each gene. See below for details. Cheng “Before clustering, the...
Cheng Li
l7501
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Feb 5, 2007
8:20 pm
2555
Hi Marc, In dchip, you can do normalization and modeling as usual (if needed use only the samples you want to cluster at an “Open group” session, since...
Cheng Li
l7501
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Feb 5, 2007
8:26 pm
2556
Dear dChip users, I am learning to use dchip for copy number, I am working onthe data of Zhao et al , Cancer research 64, 3060/ I can get the LOH and copy...
Richard Friedman
friedman@...
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Feb 6, 2007
11:44 pm
2558
Cheng and other dchip users, Upon the initial "Open Group" I got an error message to the effect, Cannot find array named 'HCC1187t' and 5 other array names are...
Richard Friedman
friedman@...
Send Email
Feb 7, 2007
5:39 pm
2559
Hi Rich, You are right about the reason. The first column of sample info file has to match exactly with the CEL file names. I probably modified some CEL file ...
Cheng Li
l7501
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Feb 7, 2007
8:40 pm
2560
Hi, When I run analysis-->genome, the analysis is completed but then it crashes when the diagram appears. How can I prevent this? Thanks, Marc ... Don't be...
M Braunstein
braunstein_m
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Feb 9, 2007
5:03 am
2561
Hi Marc and Charlotte, Thanks for letting me know the issue. This is a bug introduced in version 3/16/06+. Please use this 2/9 version to correct it: ...
Cheng Li
l7501
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Feb 9, 2007
4:45 pm
2562
Greetings, When filtering array data using analysis-->filter, in general how does one decide what limits to set for variation range, %P call, and expression...
M Braunstein
braunstein_m
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Feb 9, 2007
5:35 pm
2563
Hi, Wenbo, Thanks for letting me know the issue and providing the example files. This is a bug. Please use this 2/9 version to correct it: ...
Cheng Li
l7501
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Feb 9, 2007
6:44 pm
2564
Marc, I usually use 20% P call and 0.5 to 1.5 sd/mean to get about 500-1000 genes for clustering. Expression value magnitudes are gene dependent, so I usually ...
Cheng Li
l7501
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Feb 9, 2007
6:49 pm
2565
Hi everyone, If I could follow up on Marc's questions, if my primary interest is to get a list of genes that are changing significantly in one condition...
Fernando Silveira Mes...
mesquitafern...
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Feb 9, 2007
9:41 pm
2566
Hi, I'm using dChip to heatmap a bulk of values I obtained from uptake assay (not microarray) data, which range from single digit to 1000s values. I notice I...
virocon
Offline
Feb 10, 2007
1:21 am
2567
Jason, You may try taking log of your data (“Get external data/options/log transform…”) and then filter and cluster. If values in all rows are ...
Cheng Li
l7501
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Feb 10, 2007
11:01 pm
2570
Hi, I can't find control for 10k SNP microarrays. Where can I find this controls? Thanks Julien FERNANDEZ...
fjulienf
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Feb 13, 2007
12:39 pm
2571
Hi Julien, Ideally you want to have your own control samples processed together with tumor. Or you may try this trimmed method: ...
Cheng Li
l7501
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Feb 13, 2007
3:36 pm
2572
Dear dchip users. I am trying to reproduce the results of Zhao et al ifor copy number. During the normalize step, my job froze at HCC38t. I have the cel file ...
Richard Friedman
friedman@...
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Feb 13, 2007
10:43 pm
2573
Hi Rich, You may try either uncheck "Analysis/Normalize/View norm plot", or remove that cel/dcp file out of directory, or try this latest dchip: ...
Cheng Li
l7501
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Feb 14, 2007
12:39 am
2575
Hi Cheng, I seem to be having an issue when attempting to infer LOH using HMM (considering haplotype). In previous versions of dChip, this function would...
doctorpauln
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Feb 20, 2007
3:02 pm
2576
Dear Cheng and dChip user community, I am using the latest version dchip on the Zhao 2004 dataset and I am having trouble reading in the cel files. I get an...
Richard Friedman
friedman@...
Send Email
Feb 20, 2007
10:21 pm
2577
Hi Rich, You can check array summary file exported by dchip at "Open group". Check if the file contains the "HCC1395 june" sample. Or are only DCP files but...
Cheng Li
l7501
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Feb 20, 2007
10:31 pm
2584
Hi, I have tested the new dchip release (http://biosun1.harvard.edu/~cli/dchip2006.exe) and a problem appears when I do a linkage analysis. All steps are ok...
fjulienf
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Feb 22, 2007
1:11 pm
2585
Hi, An over question : is it normal to have a fatal ERROR when I do Merlin Linkage analysis (the stable dchip software)? AFFECTION STATISTICS ...
fjulienf
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Feb 22, 2007
1:52 pm
2586
Julien, This was a bug. Please try this 2/14 version: Http://biosun1.harvard.edu/~cli/dchip2006.exe In this version you want to toggle “Chromosome/Draw...
Cheng Li
l7501
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Feb 22, 2007
6:46 pm
2588
Ag55, Please do not use non-academic language in this group. I removed Julie’s advertising message promptly as soon as it’s posted. I have unsubscribed...
Cheng Li
l7501
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Feb 22, 2007
10:34 pm
2589
Cheng, On Feb 20, 2007, at 5:31 PM, Cheng Li wrote: > Hi Rich, > >   > > You can check array summary file exported by dchip at “Open group”. > Check...
Richard Friedman
friedman@...
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Feb 22, 2007
11:01 pm
Messages 2544 - 2589 of 2747   Oldest  |  < Older  |  Newer >  |  Newest
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