Hello Anne, We have tested dozens of readers from all over the world, and have found that the most expensive instruments are rarely the "best." My ...
3204
Cameron, Gregg
gdc2000
Dec 1, 2003 6:14 pm
Group, We have developed a system that allows us to wash tips with very good results (i.e. no carryover). We work with a lot of protein and find that the tips...
3205
Michael Ouellette
norakbio
Dec 1, 2003 8:34 pm
Gregg, We use Sigmacote (organopolysiloxane) quite often when working with peptides that are prone to non-specific binding to labware surfaces. We have seen a...
3206
lrig-discussion@yahoo...
Dec 1, 2003 9:20 pm
The Laboratory Robotics Interest Group Discussion Mail List is for comments or questions about Laboratory Robotics and Automation. You receive messages...
3207
pwfphd
Dec 1, 2003 10:28 pm
Hi Everyone, I am interested in the Tecan Power Washer 384 for cell-based assays to be used either as a stand alone instrument in a BioSafety Hood or on the...
3208
pwfphd
Dec 1, 2003 10:51 pm
Hi Everyone, I am interested in comments related to plate readers. We are looking for something that is very versatile, since it will be part of a shared core...
3209
e_matthews
Dec 2, 2003 12:39 am
Paul, BMG has a new HTS reader, The PHERAstar. I'll keep the advertisement short: It has four PMTs--two used for fluorescence intensity, two optimized for...
3210
Fabrice Turlais
fabrice_turlais
Dec 2, 2003 3:13 pm
Paul, Obviously depending in the reader some technologies might not be possible so you're goingto have to either make a compromise or go for a reader which ...
3211
Ole Sindt
murielfan2
Dec 2, 2003 3:13 pm
... Paul, we use both washers and I can recommend the Elx-405. It's way cheaper than the PW384 and we experienced several errors with the PW384 (especially...
3212
Fabrice Turlais
fabrice_turlais
Dec 2, 2003 3:13 pm
Paul, For cell based assays I would recommend the Biotek washer. The main advantage I found are the angled dispensing pins which direct the liquid to the side...
3213
G. S. Hudson
gshudson@...
Dec 2, 2003 5:21 pm
Hi Paul, I spent most of a day evaluating the Berthold fluorometer at a trade show several months ago. We read several different models of our Fluorescence...
3214
B M
baovmai
Dec 2, 2003 5:21 pm
Can someone help me troubleshoot? I have an Agilent 1100 HPLC equipped with an ELSD and DAD. I am experiencing intermittent negative peaks in my UV traces....
3215
Stevens, Joni
stevens081760
Dec 2, 2003 8:59 pm
Hello B M; If you think the culprit is air in the detector (out gassing), disconnect the outlet line from the UV detector if its attached to the ELSD and...
3216
Stevens, Joni
stevens081760
Dec 2, 2003 9:00 pm
Hello B M; One trouble shooting suggestion if you haven't done this yet is to stop the flow within the system and monitor the baseline on the UV. If you still...
3217
Kevin K. Olsen
olsenkevink
Dec 2, 2003 9:01 pm
The most common reason for negative peaks is that the UV absorbance of your sample is less than that of your mobile phase. Have you examined the spectra of...
3218
Michael_Shanler@...
Dec 2, 2003 9:04 pm
I think the new Ultra's have both top and bottom reading. -Mike Fabrice Turlais <fabrice.turlais@cambridgeant To: lrig-discussion@yahoogroups.com ...
3219
Mike.Cianci@...
Dec 3, 2003 2:35 am
Thanks Mike for the clarification. Bottom read fluorescence IS available on the new Ultra Evolution. Sincerely, Mike ... From: Michael_Shanler@......
3220
Kim Bidsted
kimbidsted
Dec 3, 2003 2:50 pm
Look at Molecular Devices, the Gemini EM is a very good reader, with a very to use software, that gives opportunity for excel data handling or to set up your...
3221
Chris Reichard
reichard_c
Dec 4, 2003 11:31 pm
I need a polyethylene 96 well UV transparent microtiter plate (for use in a spectrophotometer). Anyone know if they exist and who makes them? Thanks [Non-text...
3222
puma4me2003
Dec 5, 2003 4:37 pm
Dear Chris, don't know whether these are made from PE, but there are at least two vendors: - Greiner Bio-one UVStar ##655801, 781801 - Corning UV Microplates...
3223
Fred Spike
fred@...
Dec 5, 2003 4:37 pm
I am not aware of any PP plates that are UV transparent. Nunc, Greiner and Corning may offer plates made of other polymers that are UV transparent. We stock a...
3224
Kevin Oldenburg
matricalkevin
Dec 7, 2003 6:01 pm
Chris, We don't make a polyethylene 96 well plate but we do make a quartz bottom plate. Cost is in the $25/plate range. Cheers, Kevin ... From: Chris Reichard...
3225
pedsforth
Dec 8, 2003 2:41 pm
... use in a ... Try Thermo, part number 2205...
3226
prabakarang76
andy_zaayenga
Dec 8, 2003 2:42 pm
Hi everyone, I am Prabhakaran. I am not a serious researcher like you folks. I am medicine resident doing part time research. Hence I am running into truouble...
3227
Kevin K. Olsen
olsenkevink
Dec 8, 2003 5:18 pm
Dear Dr. Prabhakaran, While at the last LRIG vendor exhibition, I was very impressed by the Covaris E-Series sample preparation units. They work on a...
3228
joerg.dreessen@...
jdreessen2
Dec 8, 2003 5:18 pm
Dear Chris, Some years ago we developed quite acceptable UV transmitting (>340nm) 96MTPs with Polyfiltronics (now Whatman) for Fura-2 stained cellular calcium...
3229
Tom McCloud
Tom@...
Dec 8, 2003 6:43 pm
Hello, Prabakarang, I cannot claim to have experience in doing specifically what you ask about, but I have done a lot of grinding. As I see the problem, you...
3230
pwfphd
Dec 8, 2003 8:04 pm
Hi Everyone, I am interested in any comments on two thermal plate sealers: Velocity11's PlateLoc Thermal Plate Sealer Versus ABgene's ALPS 300™ automated...
3231
David Burden
david.burden@...
Dec 8, 2003 8:46 pm
Hi Prabhakaran: I think you have several options, depending on your throughput. If you are doing a limited number of samples, then a micro tissue grinder...
3232
louiseannecooper
louiseanneco...
Dec 9, 2003 1:37 pm
Hi all I am currently trying to find some filters that can be attached to the supply tubing for the ELX50 automated plate washer. Does anyone have any ideas? ...
