Thanks, Steve. Has anyone ever put a vacuum block inside a temperature controlled chamber? I am looking to try 70 C. We will probably not vary the ...
Michael_Shanler@...
Nov 1, 2006 4:05 pm
5398
Hello I would be happy if I could stay as an "alias"! I've been given the task of reducing costs in the area of tip usage. One suggestion is to wash / rinse...
Mike, 70C is on the high side. 40C can be achieved in large containments. Heat loss becomes a major issue above 40. It is an interesting problem. I suggest...
Fillers, Steve
sfillers@...
Nov 1, 2006 6:09 pm
5400
Hello, I'm new to this group. We have a Qiagen BioRobot 3000 that has hardly been used, because the software and setup to run it is not very user friendly. I...
I've written programs to wash the Biomek 2000 tips that we used to check our methods. Typically, I aspirated and dispensed water and then aspirated and...
Dear Mr. Forster: I don't use a QIAGEN BioRobot, but your discussion of the issues you are contemplating caught my interest. At the beginning, you mentioned...
Hello, In response to your post on how to possibly reduce the cost of tips, are you familiar with the Plasma tip cleaning process? This process allows you to...
Ted: Thanks for the reply. I'm hoping to use the TempliPhi reaction to help replace the way we do things now. Currently we do plasmid preps on the Autogen,...
Here is one from left field.... If I was to "semi-automate" a torque capping / uncapping of roller bottles, how would this best be done? One of our groups...
Michael_Shanler@...
Nov 2, 2006 2:49 am
5406
We're having trouble getting the integrated barcode reader on our Cytomat to read barcodes on plates with the lid on. If we put the barcode on the lid it works...
Hi Hankjam, we use a 384-well wash station on our Biomek NX for plasmid preparation and sequence reaction set-up & cleanups. We've had no problems regarding...
Hello Dr. Forster, I am the Service Manager for your account at Qiagen. We at Qiagen would definitely be intersted in helping you use your BR3000 again. With a...
Hi, I have used the Sciclone ALH tip washing station and found it to be very effective. The washer has a built in sonicator which when tested using fluoroscein...
Hello, Certainly and it is a common solution to tip washing. One thing you would need to post though is a description of what you are trying to wash off the...
I am looking for a thin seal, clear or opaque, that is penetrable with disposable tips. Can anyone suggest a seal? Rebecca Turincio Automation and Process...
Rebecca Turincio
rturincio@...
Nov 3, 2006 6:55 pm
5412
Hello Rebecca, Nunc offers several penetrable seals: 1) Clear vinyl with a pre-cut, self-sealing "X" over each well, item 236701 2) Clear polyethylene, ...
Hello Steve, Because reading through lids can sometime cause issues with barcode scanning, Nunc offers a lid with side "cut-outs" that permit direct read of...
I tried several search engines and my results may make your searching for automation solutions easier. I searched for liquid handler On Google (the search box...
Dear Steve, we have seen this problem in the field as well. It is usually because the lid cuts across the centre of the label - suggestion, if possible place...
Joni, what happens if you enter the plate with 96 tips and then withdraw - do you pick up the plate? In other words, would a regular plate with maybe 10 µl...
Hello Olaf; When we use the EZ-Pierce we do hold the plate down, because there's a chance you'll pick up the plate especially if the plate is light. They do...
Hello All, I am looking for information on 24- and 48-well microtiter plates. I am interested in finding both a 24- and a 48-well microtiter plate that will...
John Bradshaw
JBradshaw@...
Nov 9, 2006 6:09 am
5421
Please note, this is NOT a solution to the original problem. I wanted to add that on our Biomek FX we have successfully used Glad Press and Seal as a...
Hi, I have a STARlet (same thing, smaller deck space) for use for ELISA-based sample normalization and serial dilutions... AFA ease of use, I'd have to say...
John I have used both 96 and 48 well "Uniplates" from Whatman for high volume work on the Sciclone i1000. They have a 650µl 96 well plate (p/n 7701-3651)...
Hi, We are looking for a consultant to work with our small company to develop custom cherry picking scripts and standard dilution scripts with the Biomek 2000....
Dear all, I am trying to find a way to validate a serial dilution protocol, covering a bit less than 5 logs (1 to 10-5) in 100 % DMSO. Fluorescence seems to...
Hi Keith, I have worked with Polara to schedule a platform containing different devices such as Beckman dispenser, plate filtration system, incubators, barcode...
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