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#5505 From: "Ian Yates" <iyates@...>
Date: Mon Jan 22, 2007 6:21 pm
Subject: RE: Bar coding Master Plates and Daughterplates: guidelines?
iwyates
Send Email Send Email
 
Hi Hankjam,



I wasn't sure if you wanted just the labeler or a workstation to do
print-and-apply. If it's the latter then one option is our Print-And-Apply
Workstation (A BenchCel and VCode). This is used by a number of organizations to
prepare labeled plates for screening. A 4-stack BenchCel would give you capacity
for approx 150 plates. You could also add liquid handling to the system if you
wanted to.



If you're looking for a stand-alone instrument for benchtop use or to integrate
into an existing platform than the VCode itself would do the job.



If you'd like more info try our website ( www.velocity11.com
<http://www.velocity11.com/>  ).



Cheers,



Ian.



Ian Yates - Director of Applications

Velocity11 - At the speed of Discovery®

Email: iyates@... <mailto:iyates@...>

Office: +1 650 846 6657

Cell: +1 650 269 5747

www.velocity11.com <http://www.velocity11.com/>





________________________________

From: lrig-discussion@yahoogroups.com [mailto:lrig-discussion@yahoogroups.com]
On Behalf Of Andrew Johnston
Sent: Saturday, January 20, 2007 2:03 AM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Bar coding Master Plates and Daughterplates:
guidelines?



Hello

Rather than re invent the wheel for the nth time I thought I would ask this
group for recommendations for Bar coding Master Plates and Daughterplates (if
that's the correct usage) for a small scale screen such as:

80 96 well plates
Two concentrations: 1 and 10 µM FAC
Stock conc: 40 x FAC
Kinases: +200
Screening format: 384 well plates

If this has been discussed before I'm sorry. Looked through the archive and it
was taking some time to go through it!

Best wishes

Yours

Hankjam


---------------------------------
New Yahoo! Mail is the ultimate force in competitive emailing. Find out more at
the Yahoo! Mail Championships. Plus: play games and win prizes.

[Non-text portions of this message have been removed]





[Non-text portions of this message have been removed]

#5506 From: "Fillers, Steve" <sfillers@...>
Date: Mon Jan 22, 2007 7:39 pm
Subject: RE: Bar coding Master Plates and Daughterplates: guidelines?
sfillers@...
Send Email Send Email
 
Simple rules for barcodes.

1. Barcodes are de-coded by machines
2. The printed code must be readable by your machine
3. The all codes sequences must be unique
4. The codes and associated information are accessible from your database

That is it.

Recommendations for ease of operations and highest quality control:
1. Buy bar-coded microplates
2. Buy pre-printed barcodes
Note: if you are spilling enough to compromise your barcode, your samples are
likely to be compromised as well

I often agree with Neil.  His observation on label content is very true.
"in every company I have worked in this has been a source of lively discussion"

I would just add that human readable information introduces error.  If you do
not even presume to know what it says, you cannot be wrong.

Cheers!
Steve




________________________________

From: lrig-discussion@yahoogroups.com [mailto:lrig-discussion@yahoogroups.com]
On Behalf Of Andrew Johnston
Sent: Saturday, January 20, 2007 5:03 AM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Bar coding Master Plates and Daughterplates:
guidelines?



Hello

Rather than re invent the wheel for the nth time I thought I would ask this
group for recommendations for Bar coding Master Plates and Daughterplates (if
that's the correct usage) for a small scale screen such as:

80 96 well plates
Two concentrations: 1 and 10 µM FAC
Stock conc: 40 x FAC
Kinases: +200
Screening format: 384 well plates

If this has been discussed before I'm sorry. Looked through the archive and it
was taking some time to go through it!

Best wishes

Yours

Hankjam


---------------------------------
New Yahoo! Mail is the ultimate force in competitive emailing. Find out more at
the Yahoo! Mail Championships. Plus: play games and win prizes.

[Non-text portions of this message have been removed]






[Non-text portions of this message have been removed]

#5507 From: "stevenkrotzer" <steven_krotzer@...>
Date: Mon Jan 22, 2007 7:57 pm
Subject: Deep well plate lids
stevenkrotzer
Send Email Send Email
 
I am looking for a sterile 2ml, 96-well PP assay block with an "easily
removable" lid (removable by a Biomek FX liquid handler).

I am currently using a Corning Costar #3960 96-well, 2ml,PP assay
blocks along with Corning Costar universal lids.  However, this
combination is only ~70% reliable, as the lids are
occasionally difficult to remove due to plate to plate
variations/tolerances. This causes the de-lidding to fail (the entire
plate is picked up instead of just the lid).

Does anyone know of a vendor (or combination of vendors)  that will
work in this case?  I was hoping to get some samples of various lids
so that I might try them on some of the "worst case" assay blocks I
find.


Thank you in advance,
Steve

#5508 From: "Jim Batchelor" <jim.batchelor@...>
Date: Mon Jan 22, 2007 9:03 pm
Subject: RE: Deep well plate lids
jbatchelor51
Send Email Send Email
 
I use Innovative Microplates (Now seahorse labware) universal PS lids
for everything.

If you need PP lids, then...well most of them seem awfully soft.  So
gripping the lid and not squeezing the thing onto the plate is dicey.

The Innovative ones are square so they fit every plate.

I've not had any problem with our gripper taking the lids off.

jim
James D. Batchelor, Ph.D.
Caliper Life Sciences
Hopkinton, MA 01748
jim.batchelor@...
508-497-3341


________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of stevenkrotzer
Sent: Monday, January 22, 2007 2:57 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Deep well plate lids



I am looking for a sterile 2ml, 96-well PP assay block with an "easily
removable" lid (removable by a Biomek FX liquid handler).

I am currently using a Corning Costar #3960 96-well, 2ml,PP assay
blocks along with Corning Costar universal lids. However, this
combination is only ~70% reliable, as the lids are
occasionally difficult to remove due to plate to plate
variations/tolerances. This causes the de-lidding to fail (the entire
plate is picked up instead of just the lid).

Does anyone know of a vendor (or combination of vendors) that will
work in this case? I was hoping to get some samples of various lids
so that I might try them on some of the "worst case" assay blocks I
find.

Thank you in advance,
Steve




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#5509 From: "Tom Wright, T-US" <tom.wright@...>
Date: Mon Jan 22, 2007 9:25 pm
Subject: RE: Tecan PW384 containers
tom.wright@...
Send Email Send Email
 
Hi Bei Fengao ~ Tecan offers larger-capacity waste bottles for our
microplate washers, but since the bottle is all you need at this point
it may be simpler to order one directly from Fisher Scientific.   The
20-liter version of Nalgene's heavy-duty vacuum-rated carboy is
Nalgene's part number 2226-0050, available from Fisher as part number
02-960-15.   Fisher's link is
https://www1.fishersci.com/Coupon?gid=48170&cid=1328



Best of luck,

~~~ Tom ~~~

Tom Wright

Custom Applications Product Manager

_____________________________________________

nTECANn   U.S.

4022 Stirrup Creek Road, Suite # 310, Durham, NC, USA 27703

Phone: 001-(919)-361-5200, ext. 530

Fax: 001-(919)-361-5201

http://www.tecan.com

_________________________________________________________________

This message is intended for the addressee(s) only. It may contain
privileged and confidential information. Any disclosure, copying or
distribution of this message is prohibited and may be unlawful. If you
are not the intended recipient, please destroy this message and notify
us immediately. Thank you for your cooperation.



________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of bei_fengao
Sent: Monday, January 22, 2007 9:38 AM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Tecan PW384 containers



Hello, i am doing an automated assay using a PW384 plate washer from
Tecan that requires large volumes of wash buffer.I run out very
quickly and I wanted to know if anyone knew where I could get larger
(than 5 Liter) heavy duty vacuum bottles. I currently have 5L Nalgene
bottles. Thank you in advance.





[Non-text portions of this message have been removed]

#5510 From: "bum94587" <bum94587@...>
Date: Mon Jan 22, 2007 11:48 pm
Subject: Re: Bar coding Master Plates and Daughterplates: guidelines?
bum94587
Send Email Send Email
 
> Rather than re invent the wheel for the nth time I thought I
would ...

great idea!

There are pretty good general guideline on barcodes, including this
link from computype:

http://www.computype.com/pdfs/LabAutomation_2003.pdf
... and of course, there are short courses:
http://labautomation.org/LA/LA07/shortcourses.php

but, if you're still interested in a very simple, crude rules from
"just another guy in the lab", here's what i would say:

1. Do not use cheap printers/labels - (please, no avery labels!)
professional quality label printers like zebra TLP 3244 are quite
affordable, and so is buying pre-printed labels.

2. Do not make your barcode length variable. - i.e. set fixed number
characters for bc-id... like P00009,P000012, etc ... do not go P1,
P2, P12, P1234. (I think this is rather obivous, but I have seen
some people do this ... this is really a bad idea, unless you know
what you're doing)

3. On the "Hotly debated"- straight-id vs. meaningful id vs. id +
info.", I think the debate is a good thing - provided people don't
end up killing each other... but the tendency is that most
scientists will argue for more info. on the plate (either within-the
unique id or as an added 'alias')... in your case, many might argue
that the "mother" plates' id should be something like "M00001", and
it's daughter "D00001-01 to D00001-10", etc... this is no big deal
for small set of plates, but rather problematic for larger sets of
plates in the long run...especially if you are also going to have a
relational database to track the relationship - in that case, this
is flat-out bad design,no room for debate here. All tracking ought
to be done at the database level, and not by the labels. Now, as for
putting EXTRA info. besides a barcode to help scientiest identify
the plates easily depends a largely on your environment (proudctionn
vs. R&D), and hence a debate is good.

hope this helps!

rob pak
Codexis Inc.

--- In lrig-discussion@yahoogroups.com, Andrew Johnston
<hankjam@...> wrote:
>
> Hello
>
> Rather than re invent the wheel for the nth time I thought I would
ask this group for recommendations for Bar coding Master Plates and
Daughterplates (if that's the correct usage) for a small scale
screen such as:
>
> 80 96 well plates
> Two concentrations: 1 and 10 µM FAC
> Stock conc: 40 x FAC
> Kinases: +200
> Screening format: 384 well plates
>
> If this has been discussed before I'm sorry. Looked through the
archive and it was taking some time to go through it!
>
> Best wishes
>
> Yours
>
> Hankjam
>
>
> ---------------------------------
>  New Yahoo! Mail is the ultimate force in competitive emailing.
Find out more at the Yahoo! Mail Championships. Plus: play games and
win prizes.
>
> [Non-text portions of this message have been removed]
>

#5511 From: "jbio1" <jbio1@...>
Date: Tue Jan 23, 2007 1:04 am
Subject: Re: Tip washing: can it be done?
jbio1
Send Email Send Email
 
Hankjam,

What about eliminating the tips and washing all together out of one
or more steps in the process?  Our ECHO systems may be a good option
to consider.

You can take a closer look at www.labcyte.com and contact us offline
if you'd like more info.

Regards,
Justin

--- In lrig-discussion@yahoogroups.com, "atopp2" <atopp@...> wrote:
>
> --- In lrig-discussion@yahoogroups.com, "hankjam" <hankjam@> wrote:
> >
> > Hello
> >
> > I would be happy if I could stay as an "alias"!
> >
> > I've been given the task of reducing costs in the area of tip
usage.
> >
> > One suggestion is to wash / rinse tips. The idea does not thrill
me! I
> > was wondering if anyone on this DG has any experience with the
> > following and cares to share them, I would be grateful.
> >
> > Biomek 2000
> > Sciclone ALH3000
> > Platemate
> > Mosquito
> >
> > Many thanks for your time.
> >
> > Hankjam
> >
>
> Hi Hankjam
>
> we use different pipettors were we reuse tips. We normally pipette
> DMSO or protein solutions and need to be sure to have no crosstalk,
> blocked tips and good CV. This is not the easiest task.
> Here some lessons we learned:
> 1) Avoid washing into the chimney of the washing position. This is
not
> a real washing only a dilution. Then check that enough solvent
comes
> through the chimney to wash the tip also from the outside without
> going inside while aspirating.
> 2) To avoid crosstalk we have checked the washing with Rhodamin,
> Tartrazin and Fluoresceine for remaining absorbtion resp.
> fluorescence. No signals with each of the dyes helps avoiding
> crosstalk and show accurate washing. We also implement a "PAMPERS"
> step after washing with blowout lowe rthe zero point of the
pippettor
> to get rid of the liquid inside the tip.
> 3) blocked tips: precipitation could block your tip and therefore
no
> compound transfered afterwards (or if it is a hit compound you will
> have hits over the next 100 plates). Therefore we check the plates
> manually if something is inside (easy for us because we pipette
into
> dry plates). I know sites were they check this with a CCD camera
for
> each plate)
> 4) good CV: wash the tips before making the starting CV. Then you
know
> the settings for "used" tips.
> 5) with some plastic tips you could have difficulties with sticking
> proteins. Sometimes saturation with BSA could help sometimes you
have
> to run with the uses protein and reach saturation and sometimes you
> have no chance and you have to change tips after each pipetting
steps.
>
> Hope this could help and show the not trivial washing of tips.
>
> Best regards
>
> Andreas
>

#5512 From: "Kevin K. Olsen" <olsenk@...>
Date: Tue Jan 23, 2007 1:50 pm
Subject: Bar coding Master Plates and Daughterplates
olsenkevink
Send Email Send Email
 
Steve Fillers made some excellent points when he wrote that:


1. Barcodes are de-coded by machines
2. The printed code must be readable by your machine
3. The all codes sequences must be unique
4. The codes and associated information are accessible from your database


If there is one item I would like to add to this list it would be to
purchase a bar code verifier.


A verifier is a special bar code reader that gives the label a grade
based on the ANSI standard for that particular code.


The verifier will evaluate things like the edge contrast beween the bar
and space characters, the size of the "quiet zone" on the ends of the
bars, and whether your bar characters are completely dark or if there
are "holes" in them.


For a few hundred dollars a verifier can save you lots of time and trouble.


Many scientists (who should know better) make the mistake of thinking
that they can simply scan the bar code with a reader and "test" the
label.  All that this "test" has demonstrated that this particular
label, with this particular scanner, read with this particular operator
was able to read the label on this particular day.


The ordinary scanner can't tell you if the label quality is good or bad
and if it is bad there is no guarantee that the next label printed will
be readable.  (Please don't ask me how I know this.)


Your bar code equipment supplier will be able to set you up with a good
verifier.  If you work in an FDA regulated facility there are already
excellent label verifiers available.


If your supplier says that you don't need a verifier, find another supplier.

Have fun,


Kevin Olsen
Instrumentation Specialist
Chemistry and Biochemistry Support Staff
Montclair State Univertsity
Montclair, NJ, 07043
973-655-4076



[Non-text portions of this message have been removed]

#5513 From: "Fonzie" <jaiver.alfonso@...>
Date: Tue Jan 23, 2007 5:16 pm
Subject: Re: Tecan PW384 containers
fonziekiss
Send Email Send Email
 
I use the 20L Nalgenes for all my washers. they work great and you
can even tandem link them for high throughput operations.


--- In lrig-discussion@yahoogroups.com, "Tom Wright, T-US"
<tom.wright@...> wrote:
>
> Hi Bei Fengao ~ Tecan offers larger-capacity waste bottles for our
> microplate washers, but since the bottle is all you need at this
point
> it may be simpler to order one directly from Fisher Scientific.
The
> 20-liter version of Nalgene's heavy-duty vacuum-rated carboy is
> Nalgene's part number 2226-0050, available from Fisher as part
number
> 02-960-15.   Fisher's link is
> https://www1.fishersci.com/Coupon?gid=48170&cid=1328
>
>
>
> Best of luck,
>
> ~~~ Tom ~~~
>
> Tom Wright
>
> Custom Applications Product Manager
>
> _____________________________________________
>
> nTECANn   U.S.
>
> 4022 Stirrup Creek Road, Suite # 310, Durham, NC, USA 27703
>
> Phone: 001-(919)-361-5200, ext. 530
>
> Fax: 001-(919)-361-5201
>
> http://www.tecan.com
>
> _________________________________________________________________
>
> This message is intended for the addressee(s) only. It may contain
> privileged and confidential information. Any disclosure, copying or
> distribution of this message is prohibited and may be unlawful. If
you
> are not the intended recipient, please destroy this message and
notify
> us immediately. Thank you for your cooperation.
>
>
>
> ________________________________
>
> From: lrig-discussion@yahoogroups.com
> [mailto:lrig-discussion@yahoogroups.com] On Behalf Of bei_fengao
> Sent: Monday, January 22, 2007 9:38 AM
> To: lrig-discussion@yahoogroups.com
> Subject: [lrig-discussion] Tecan PW384 containers
>
>
>
> Hello, i am doing an automated assay using a PW384 plate washer
from
> Tecan that requires large volumes of wash buffer.I run out very
> quickly and I wanted to know if anyone knew where I could get
larger
> (than 5 Liter) heavy duty vacuum bottles. I currently have 5L
Nalgene
> bottles. Thank you in advance.
>
>
>
>
>
> [Non-text portions of this message have been removed]
>

#5514 From: martin wang <pri_martin@...>
Date: Tue Jan 23, 2007 5:10 pm
Subject: Re: Bar coding Master Plates and Daughterplates
pri_martin
Send Email Send Email
 
Also keep in mind of your work load when selecting a printer. Portable small
unit, such as LabXpert, can print less than 300 labels per day. Otherwise you
will burn the printer head. 600 dpi thermal benchtop printer can make a few
thousands labels per day.

Martin

--
Martin J Wang
WIS Biomed
120 Boothbay Avenue
San Mateo, CA 94404
650-242-8368 T
650-281-7924 C
650-242-8244 F
mwang@...

http://www.wisbiomed.com

----- Original Message ----
From: Kevin K. Olsen <olsenk@...>
To: lrig-discussion@yahoogroups.com
Sent: Tuesday, January 23, 2007 5:50:50 AM
Subject: [lrig-discussion] Bar coding Master Plates and Daughterplates













             Steve Fillers made some excellent points when he wrote that:



1. Barcodes are de-coded by machines

2. The printed code must be readable by your machine

3. The all codes sequences must be unique

4. The codes and associated information are accessible from your database



If there is one item I would like to add to this list it would be to

purchase a bar code verifier.



A verifier is a special bar code reader that gives the label a grade

based on the ANSI standard for that particular code.



The verifier will evaluate things like the edge contrast beween the bar

and space characters, the size of the "quiet zone" on the ends of the

bars, and whether your bar characters are completely dark or if there

are "holes" in them.



For a few hundred dollars a verifier can save you lots of time and trouble.



Many scientists (who should know better) make the mistake of thinking

that they can simply scan the bar code with a reader and "test" the

label.  All that this "test" has demonstrated that this particular

label, with this particular scanner, read with this particular operator

was able to read the label on this particular day.



The ordinary scanner can't tell you if the label quality is good or bad

and if it is bad there is no guarantee that the next label printed will

be readable.  (Please don't ask me how I know this.)



Your bar code equipment supplier will be able to set you up with a good

verifier.  If you work in an FDA regulated facility there are already

excellent label verifiers available.



If your supplier says that you don't need a verifier, find another supplier.



Have fun,



Kevin Olsen

Instrumentation Specialist

Chemistry and Biochemistry Support Staff

Montclair State Univertsity

Montclair, NJ, 07043

973-655-4076



[Non-text portions of this message have been removed]














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#5515 From: Marlene <ytrewq84@...>
Date: Fri Jan 26, 2007 9:14 pm
Subject: Does methanol get rid of proteins in tubing?
ytrewq84
Send Email Send Email
 
Hi all,

I have been asked to use methanol to clean the tygon
tubing on our Multidrop instead of ethanol in order to
save money.  I dispense 2nM protein + beads solution
through it.  I have always used ethanol.

Will methanol serve as well?

Thanks in advance.




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#5516 From: "Tom Wright, T-US" <tom.wright@...>
Date: Tue Jan 30, 2007 2:44 pm
Subject: RE: Re: Tecan PW384 containers
tom.wright@...
Send Email Send Email
 
Fonzie, good point about tandem-linking vacuum waste bottles to double
or triple your capacity (& your walkaway time).   One piece of advice
though to keep your washer's aspiration performance uniform throughout
your automated run; your waste bottles should be set up in parallel, not
in series.



If they're "daisy-chained" (such that you're pulling vacuum for the
second bottle through the first) you'll see a dramatic drop in
aspiration rate & efficiency when the first bottle is filled because the
pump then needs to pull fluid across the "bridge" tubing rather than
air.   Avoid this differential by having tubing splitters on both the
inlet & outlet sides of the waste bottles so vacuum is being pulled
through all bottles simultaneously in parallel and they all fill at the
same rate.



Happy Automating,



Tom Wright

Custom Applications Product Manager

Tecan Integration Group, U.S.

________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of Fonzie
Sent: Tuesday, January 23, 2007 12:17 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Re: Tecan PW384 containers



I use the 20L Nalgenes for all my washers. they work great and you
can even tandem link them for high throughput operations.

--- In lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com> , "Tom Wright, T-US"
<tom.wright@...> wrote:
>
> Hi Bei Fengao ~ Tecan offers larger-capacity waste bottles for our
> microplate washers, but since the bottle is all you need at this
point
> it may be simpler to order one directly from Fisher Scientific.
The
> 20-liter version of Nalgene's heavy-duty vacuum-rated carboy is
> Nalgene's part number 2226-0050, available from Fisher as part
number
> 02-960-15. Fisher's link is
> https://www1.fishersci.com/Coupon?gid=48170&cid=1328
<https://www1.fishersci.com/Coupon?gid=48170&cid=1328>
>
>
>
> Best of luck,
>
> ~~~ Tom ~~~
>
> Tom Wright
>
> Custom Applications Product Manager
>
> _____________________________________________
>
> nTECANn U.S.
>
> 4022 Stirrup Creek Road, Suite # 310, Durham, NC, USA 27703
>
> Phone: 001-(919)-361-5200, ext. 530
>
> Fax: 001-(919)-361-5201
>
> http://www.tecan.com <http://www.tecan.com>
>
> __________________________________________________________
>
> This message is intended for the addressee(s) only. It may contain
> privileged and confidential information. Any disclosure, copying or
> distribution of this message is prohibited and may be unlawful. If
you
> are not the intended recipient, please destroy this message and
notify
> us immediately. Thank you for your cooperation.
>
>
>
> ________________________________
>
> From: lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com>
> [mailto:lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com> ] On Behalf Of bei_fengao
> Sent: Monday, January 22, 2007 9:38 AM
> To: lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com>
> Subject: [lrig-discussion] Tecan PW384 containers
>
>
>
> Hello, i am doing an automated assay using a PW384 plate washer
from
> Tecan that requires large volumes of wash buffer.I run out very
> quickly and I wanted to know if anyone knew where I could get
larger
> (than 5 Liter) heavy duty vacuum bottles. I currently have 5L
Nalgene
> bottles. Thank you in advance.
>
>
>
>
>
> [Non-text portions of this message have been removed]
>





[Non-text portions of this message have been removed]

#5517 From: "Frittrang, Alexander" <Alexander.Frittrang@...>
Date: Tue Jan 30, 2007 4:10 pm
Subject: AW: Does methanol get rid of proteins in tubing?
frittrang02
Send Email Send Email
 
Hello Marlene,



ethanol is usually used to precipitate proteins. So ethanol won't help at all.
Methanol is known to be a powerfull solvent, but I would expect its impact on
proteins may be similar as ethanol (after all its chemically related). A
solution of 0.05 mMolar NaOH is known to solubilize proteins very effectively. I
have used it for liquid handlers before and did not observe any problems



with kind regards

Alex



-----Ursprüngliche Nachricht-----
Von: lrig-discussion@yahoogroups.com [mailto:lrig-discussion@yahoogroups.com] Im
Auftrag von Marlene
Gesendet: Freitag, 26. Januar 2007 22:15
An: lrig-discussion@yahoogroups.com
Betreff: [lrig-discussion] Does methanol get rid of proteins in tubing?



Hi all,

I have been asked to use methanol to clean the tygon
tubing on our Multidrop instead of ethanol in order to
save money. I dispense 2nM protein + beads solution
through it. I have always used ethanol.

Will methanol serve as well?

Thanks in advance.

__________________________________________________________
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Get new email alerts with the free Yahoo! Toolbar.
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#5518 From: lrig-discussion@yahoogroups.com
Date: Thu Feb 1, 2007 10:38 am
Subject: File - lrig-discussion Periodic Message.txt
lrig-discussion@yahoogroups.com
Send Email Send Email
 
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#5519 From: "Rodriguez, Richard" <Richard.Rodriguez@...>
Date: Thu Feb 1, 2007 6:38 pm
Subject: RE: Does methanol get rid of proteins in tubing?
Richard.Rodriguez@...
Send Email Send Email
 
Hello Marlene,

We have used a wash protocol with much success in cleaning multidrop
tubing that have dispensed protein solutions thru it.
1. Flush with water
2. Flush with an enzymatic detergent (CoulterClenz) Perhaps allow it to
sit for about 10 minutes or more to allow the enzymes to work.
3. Flush with water to dilute
4. Flush with 70% reagent alcohol to remove residual detergent
5. Flush with water

With regards to the beads, are they carbohydrate in nature? Our wash
protocol and the one you described tend to cause precipitation and clog
the tips.
To get around that we had to back flush the tubing in an acidic
solution.

Hope this helps,
Richard Rodriguez
Research Automation Technology Manager

MDS PharmaServices, Inc.
Pharmacology Services
22011 30th Drive SE
Bothell, WA 98021-4444

Richard.Rodriguez@...




________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of Marlene
Sent: Friday, January 26, 2007 1:15 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Does methanol get rid of proteins in tubing?



Hi all,

I have been asked to use methanol to clean the tygon
tubing on our Multidrop instead of ethanol in order to
save money. I dispense 2nM protein + beads solution
through it. I have always used ethanol.

Will methanol serve as well?

Thanks in advance.

__________________________________________________________
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[Non-text portions of this message have been removed]

#5520 From: Marlene <ytrewq84@...>
Date: Fri Feb 2, 2007 6:14 pm
Subject: RE: Does methanol get rid of proteins in tubing?
ytrewq84
Send Email Send Email
 
Thank you Richard.  You have many suggestions that are
new to me so I am anxious to try them.

We are doing an Alphascreen.  I don't know if those
are carbohydrate beads or not.

Kindest regards,
Marlene
--- "Rodriguez, Richard"
<Richard.Rodriguez@...> wrote:

> Hello Marlene,
>
> We have used a wash protocol with much success in
> cleaning multidrop
> tubing that have dispensed protein solutions thru
> it.
> 1. Flush with water
> 2. Flush with an enzymatic detergent (CoulterClenz)
> Perhaps allow it to
> sit for about 10 minutes or more to allow the
> enzymes to work.
> 3. Flush with water to dilute
> 4. Flush with 70% reagent alcohol to remove residual
> detergent
> 5. Flush with water
>
> With regards to the beads, are they carbohydrate in
> nature? Our wash
> protocol and the one you described tend to cause
> precipitation and clog
> the tips.
> To get around that we had to back flush the tubing
> in an acidic
> solution.
>
> Hope this helps,
> Richard Rodriguez
> Research Automation Technology Manager
>
> MDS PharmaServices, Inc.
> Pharmacology Services
> 22011 30th Drive SE
> Bothell, WA 98021-4444
>
> Richard.Rodriguez@...
>
>
>
>
> ________________________________
>
> From: lrig-discussion@yahoogroups.com
> [mailto:lrig-discussion@yahoogroups.com] On Behalf
> Of Marlene
> Sent: Friday, January 26, 2007 1:15 PM
> To: lrig-discussion@yahoogroups.com
> Subject: [lrig-discussion] Does methanol get rid of
> proteins in tubing?
>
>
>
> Hi all,
>
> I have been asked to use methanol to clean the tygon
> tubing on our Multidrop instead of ethanol in order
> to
> save money. I dispense 2nM protein + beads solution
> through it. I have always used ethanol.
>
> Will methanol serve as well?
>
> Thanks in advance.
>
>
__________________________________________________________
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> contain privileged or confidential information and
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#5521 From: "Kevin K. Olsen" <olsenk@...>
Date: Fri Feb 2, 2007 2:06 pm
Subject: Liquid Scintillation Counters for microplate use
olsenkevink
Send Email Send Email
 
It has been more than a year since I posted a request on this list for
suggestions about a liquid scintillation counter for microplate use.  As
it sometimes happens the project was put on hold and we never purchased
the unit.

The project has started up again and it is time to do some shopping.

We are looking for a liquid scintillation counter that is comparable to
the old Beckman  LS 3801.

The health and safety people need to analyze swipes, the biochemists
need to read microplates.

Is there any instrument that can do both?

The Packard Top Count looks well suited to our needs but can it handle
these two disparate applications?

Thank you,

Kevin Olsen
Instrumentation Specialist
Chemistry and Biochemistry Support Staff
Montclair State University
Montclair, NJ, 07043
973-655-4076



[Non-text portions of this message have been removed]

#5522 From: "ldseger" <ldseger@...>
Date: Fri Feb 9, 2007 10:17 pm
Subject: Who makes an automated liquid sampler?
ldseger
Send Email Send Email
 
My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?

#5523 From: Dan Leggate <d.leggate@...>
Date: Sat Feb 10, 2007 7:47 pm
Subject: Re: Does methanol get rid of proteins in tubing?
drleggate
Send Email Send Email
 
A word of warning about CoulterClenz (speaking from bitter experience).
You need to be very careful that it is all removed as any residual
enzyme will ruin your next assay. I also used an acid rinse afterwards
which did the trick.

I've also used a Multidrop to dispense beads (sepharose) and found it to
work well until beads eventually clog a tip. Then even with thorough
flushing to remove the beads I always found that the tip would clog
again the next time I used it. I haven't tried using acid though so will
give it a go.

Best wishes,
Dan

Rodriguez, Richard wrote:
>
>
> Hello Marlene,
>
> We have used a wash protocol with much success in cleaning multidrop
> tubing that have dispensed protein solutions thru it.
> 1. Flush with water
> 2. Flush with an enzymatic detergent (CoulterClenz) Perhaps allow it to
> sit for about 10 minutes or more to allow the enzymes to work.
> 3. Flush with water to dilute
> 4. Flush with 70% reagent alcohol to remove residual detergent
> 5. Flush with water
>
> With regards to the beads, are they carbohydrate in nature? Our wash
> protocol and the one you described tend to cause precipitation and clog
> the tips.
> To get around that we had to back flush the tubing in an acidic
> solution.
>
> Hope this helps,
> Richard Rodriguez
> Research Automation Technology Manager
>
> MDS PharmaServices, Inc.
> Pharmacology Services
> 22011 30th Drive SE
> Bothell, WA 98021-4444
>
> Richard.Rodriguez@... <mailto:Richard.Rodriguez%40mdsinc.com>
>
> ________________________________
>
> From: lrig-discussion@yahoogroups.com
> <mailto:lrig-discussion%40yahoogroups.com>
> [mailto:lrig-discussion@yahoogroups.com
> <mailto:lrig-discussion%40yahoogroups.com>] On Behalf Of Marlene
> Sent: Friday, January 26, 2007 1:15 PM
> To: lrig-discussion@yahoogroups.com
> <mailto:lrig-discussion%40yahoogroups.com>
> Subject: [lrig-discussion] Does methanol get rid of proteins in tubing?
>
> Hi all,
>
> I have been asked to use methanol to clean the tygon
> tubing on our Multidrop instead of ethanol in order to
> save money. I dispense 2nM protein + beads solution
> through it. I have always used ethanol.
>
> Will methanol serve as well?
>
> Thanks in advance.
>
> __________________________________________________________
> It's here! Your new message!
> Get new email alerts with the free Yahoo! Toolbar.
> http://tools.search.yahoo.com/toolbar/features/mail/
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> <http://tools.search.yahoo.com/toolbar/features/mail/>>
>
> This email and any files transmitted with it may contain privileged or
> confidential information and may be read or used only by the intended
> recipient. If you are not the intended recipient of the email or any of
> its attachments, please be advised that you have received this email in
> error and any use, dissemination, distribution, forwarding, printing or
> copying of this email or any attached files is strictly prohibited. If
> you have received this email in error, please immediately purge it and
> all attachments and notify the sender by reply email or contact the
> sender at the number listed.
>
> [Non-text portions of this message have been removed]
>
>

#5524 From: "Stevens, Joni" <jstevens@...>
Date: Sat Feb 10, 2007 5:13 pm
Subject: RE: Who makes an automated liquid sampler?
stevens081760
Send Email Send Email
 
Hello;



We make a series of automated liquid handlers that may fit your needs.
If there is an entry port to insert the probe into the reactor we can
definitely sample at any specified interval(s) and deposit into septum
sealed vials.  If you're interested in a simple software program to
operate the automated liquid handler we also have the ability to offer
custom programs.  In addition to the custom program we also offer custom
racks designed to your specifications to hold your reactor and vials.
Please feel free to peruse our web site www.gilson.com
<http://www.gilson.com/> , "products", "automated liquid handlers".
The link below is just one of the several ALHs we offer.  Please free to
contact me for any additional info or questions, at my e-mail address
listed below.





Hope this help...have a great weekend!



Regards//joni



Joan M. Stevens, Ph.D.

Applications Laboratory Manager

Gilson, Inc







jstevens@...

1-800-445-7661 x 3291

Fax 1-608-831-4451



3000  Parmenter Street

Middleton, WI 53562

________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of ldseger
Sent: Friday, February 09, 2007 4:17 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Who makes an automated liquid sampler?



My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?





[Non-text portions of this message have been removed]

#5525 From: "Jeff Aufmann, T-US" <Jeff.Aufmann@...>
Date: Sat Feb 10, 2007 4:32 pm
Subject: RE: Who makes an automated liquid sampler?
Jeff.Aufmann@...
Send Email Send Email
 
Tecan may be able to provide such a device.  I would need more details
to confirm.  I would recommend you contact your local representative to
discuss.



Jeff Aufmann

Midwest Regional Sales Manager

lTECANl

800-352-5128

www.tecan.com





________________________________

From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of ldseger
Sent: Friday, February 09, 2007 4:17 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Who makes an automated liquid sampler?



My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?





[Non-text portions of this message have been removed]

#5526 From: amerhaj@...
Date: Sun Feb 11, 2007 1:30 am
Subject: Re: Who makes an automated liquid sampler?
amer_elhage
Send Email Send Email
 
Couple of questions on the sample state and the container.
What's the physical state of you Polymers in those reactor?
How would one sample (access) the Polymer in the SS sealed (20psig) reactors?

Regards,

Amer El-Hage
Principal
Beeston Engineering Consulting
415-314-9267
650-854-7419 (fax)
amerhaj@...


-----Original Message-----
From: ldseger@...
To: lrig-discussion@yahoogroups.com
Sent: Fri, 9 Feb 2007 2:17 PM
Subject: [lrig-discussion] Who makes an automated liquid sampler?


My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
The LRIG Discussion Group is a feature of the
Laboratory Robotics Interest Group.
http://www.lab-robotics.org/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Yahoo! Groups Links



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#5527 From: "julian_a_cox" <jacox@...>
Date: Sun Feb 11, 2007 4:28 pm
Subject: Re: Who makes an automated liquid sampler?
julian_a_cox
Send Email Send Email
 
--- In lrig-discussion@yahoogroups.com, "ldseger" <ldseger@...> wrote:
>
> My company makes polymers in a sealed, low pressure (20psig)
stainless
> steel reactor. I need to automatically sample approx 1-3 mLs from
the
> reactor at periodic times (i.e. 15 minutes), and deposit the sample
> into a septum sealed vial for analysis later.
>
> I have been looking for months to identify a company who sells such
a
> device. Is anyone out there familiar with a possible solution?
>

Hello, - we've had some experiences with similar applications over
the years.

Typically there are issues in overcoming viscosity, temperature,
pressure, adding a reagent to stop the reaction, and with the
effective cleaning of the probe and liquid path between samplings.

We're a custom automation supplier, so if you're seeking an "off the
shelf" solution, likely we're not the best answer.
However, "off the shelf" solutions might be tough to come by in this
regard.

Personally suggest you evaluate both Symyx and Chemspeed as potential
suppliers. If neither have the solution you need - please do call us
for a follow-on discussion.

ThankYou & Good Luck!

J.A. 'Tony' Cox
Sirius Automation Inc.
Buffalo Grove IL 60089
www.siriusautomation.com

'Creative Enabling Technologies. Effective Tailored Solutions'

#5528 From: "invetech_pjd" <pjd@...>
Date: Mon Feb 12, 2007 6:07 am
Subject: LAS compliance
invetech_pjd
Send Email Send Email
 
Hello All.

My name is Phillip Duncan and I am a design consultant with Invetech
Pty Ltd in Australia.

I'm often involved in projects that involve designing instrumentation
and consumables (tips & plates) for drug discovery.  One of the
recurring requirements is for the instrumentation is LAS compatible,
so I'm looking for as much information as possible on MECHANICAL
interfaces to LAS components such as robot grippers, hotels and
carousels.

My aim is to develop some design guidelines for my team of engineers,
so that we can design in adequate access clearance on instrument
decks, and we design consumables so that they can be handled by
robotics.

I'd like to cover the requirements for 80-90% of the robots, hotels
and carousels in the market.

If you have some information that you think may assist me in my goal,
please respond to this mail.

Thanks.

#5529 From: "paul murphy" <pmu@...>
Date: Mon Feb 12, 2007 2:57 pm
Subject: Re: LAS compliance
murfboston2004
Send Email Send Email
 
Hello Phillip,

You can find information on incubators/hotels such as interface software and CAD
files at www.liconic.com . Example: go to products, pick automated incubators,
pick STX 40, go to bottom of the page a pick downloads, then choose the
information you are looking for. If you have any questions you can contact me
directly.

Sincerely,

Paul Murphy
LiCONiC US, Inc.
1 Presidential Way, 104B
Woburn, MA 01801

pmu@...
www.liconic.com

781-933-2050 phone
781-933-2260 fax
   ----- Original Message -----
   From: invetech_pjd
   To: lrig-discussion@yahoogroups.com
   Sent: Monday, February 12, 2007 1:07 AM
   Subject: [lrig-discussion] LAS compliance


   Hello All.

   My name is Phillip Duncan and I am a design consultant with Invetech
   Pty Ltd in Australia.

   I'm often involved in projects that involve designing instrumentation
   and consumables (tips & plates) for drug discovery. One of the
   recurring requirements is for the instrumentation is LAS compatible,
   so I'm looking for as much information as possible on MECHANICAL
   interfaces to LAS components such as robot grippers, hotels and
   carousels.

   My aim is to develop some design guidelines for my team of engineers,
   so that we can design in adequate access clearance on instrument
   decks, and we design consumables so that they can be handled by
   robotics.

   I'd like to cover the requirements for 80-90% of the robots, hotels
   and carousels in the market.

   If you have some information that you think may assist me in my goal,
   please respond to this mail.

   Thanks.





[Non-text portions of this message have been removed]

#5530 From: "David Ley" <david.ley@...>
Date: Mon Feb 12, 2007 3:20 pm
Subject: RE: Who makes an automated liquid sampler?
david.ley@...
Send Email Send Email
 
Hi



You might try New Brunswick Scientific many years ago they had a device
called an MX3 autosampler (I think) which was designed to sample fractions
from industrial fermenters on a timed basis. I don't know if they still do
this, if not you might try one of the other fermentation tank manufacturers
who should be able to offer you something to do this.



If I have understood your mail correctly then I think this is the type of
device you are looking for.



Best Regards



David



David Ley

Head of Global Sales

Process Analysis & Automation

mail david.ley@...

web http://www.paa.co.uk

tel:  +44 (0) 1252 373000

mobile: +44 (0) 7725 762 010



Process Analysis & Automation are registered in the UK as UK2749962



   _____


From: lrig-discussion@ <mailto:lrig-discussion%40yahoogroups.com>
yahoogroups.com
[mailto:lrig-discussion@ <mailto:lrig-discussion%40yahoogroups.com>
yahoogroups.com] On Behalf Of ldseger
Sent: Friday, February 09, 2007 4:17 PM
To: lrig-discussion@ <mailto:lrig-discussion%40yahoogroups.com>
yahoogroups.com
Subject: [lrig-discussion] Who makes an automated liquid sampler?

My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?

[Non-text portions of this message have been removed]





[Non-text portions of this message have been removed]

#5531 From: Neil Benn <benn@...>
Date: Mon Feb 12, 2007 6:59 pm
Subject: LabAuto Lounge
neil_david_b...
Send Email Send Email
 
Hello,

Well sooner or later someone had to do it (apologies if I am not the
       first), I have started a blog about laboratory automation and all
       things related. If you are interested then please feel free to
       take a read and make a few comments - the url is
       http://labauto-lounge.com . It is early days so if you encounter
       any technical problems then please let me know - there will be a
       small prize for the 50th person to email me with any problems (not
       really - that's a joke).

Cheers,

Neil

#5532 From: "Tom Strader" <tom.strader@...>
Date: Tue Feb 13, 2007 1:29 pm
Subject: RE: Who makes an automated liquid sampler?
ts_8675309
Send Email Send Email
 
Hi Ldseger:

That's cakewalk except for the septum piercing part. Joni's right, the
venerable Gilson 215 was designed specifically to pierce septa in
Vacutainer (blood) tubes.  You need a strong straight probe with a guide
to keep it from bending and a fairly sharp tip that won't core (and
clog).  There are a gazillion different probes available for the 215 as
well.  Also, the sample tube needs to stay put when you pull the probe
out.  Here's a link:

http://www.gilson.com/Products/product.asp?pID=41

Cheers,
Tom Strader
Midwest LRIG Inc.
www.midwestlrig.com <http://www.midwestlrig.com/>
Nimblegen Systems Inc.
www.nimblegen.com <http://www.nimblegen.com/>



-----Original Message-----
From: lrig-discussion@yahoogroups.com
[mailto:lrig-discussion@yahoogroups.com] On Behalf Of ldseger
Sent: Friday, February 09, 2007 4:17 PM
To: lrig-discussion@yahoogroups.com
Subject: [lrig-discussion] Who makes an automated liquid sampler?

My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?



[Non-text portions of this message have been removed]

#5533 From: "Larry Chin" <lchin@...>
Date: Wed Feb 14, 2007 4:34 pm
Subject: Small volume sampling system
lchinhatch
Send Email Send Email
 
Hello,



My company produces customized instrument and equipment solutions for
liquid handling and processing.  I believe that we could come up with a
fairly simple and effective solution for this application using a base
level sampler with a valve control system.  Let me know if we can help.



Thank you,



Larry Chin

Vice President of Business Development



Hatch Technology

580 Commerce Drive

Fall River, MA 02720

508-673-9555 Ph

508-673-9550 Fax







From: lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com>
[mailto:lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com> ] On Behalf Of ldseger
Sent: Friday, February 09, 2007 4:17 PM
To: lrig-discussion@yahoogroups.com
<mailto:lrig-discussion%40yahoogroups.com>
Subject: [lrig-discussion] Who makes an automated liquid sampler?

My company makes polymers in a sealed, low pressure (20psig) stainless
steel reactor. I need to automatically sample approx 1-3 mLs from the
reactor at periodic times (i.e. 15 minutes), and deposit the sample
into a septum sealed vial for analysis later.

I have been looking for months to identify a company who sells such a
device. Is anyone out there familiar with a possible solution?

[Non-text portions of this message have been removed]







www.hatchtechnology.com





[Non-text portions of this message have been removed]

#5534 From: "xinoci78" <alee@...>
Date: Thu Feb 15, 2007 6:43 pm
Subject: Solubility testing methods
xinoci78
Send Email Send Email
 
Hello,

I am currently investigating methods for high-throughput solubility
testing.  I would appreciate any and all comments/advice regarding
your own experiences with this kind of assay.  All methods you may
have used are of interest (shake flask, turbidimetry, etc). If you are
aware of any print/internet resources, I would appreciate hearing
about those, as well.


Thank you,
Adrianne

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