qPCR NEWS June 2009 - MIQE media & press review
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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time PCR (qPCR
and qRT-PCR), which are compiled and summarised on the Gene Quantification
homepage. The focus of this newsletter issue is:

- MIQE - media & press review
- For better navigation we developed a TAG CLOUD =>
http://directory.gene-quantification.info/
- NEW qPCR / real-time PCR BLOG => http://real-time-pcr.blogspot.com/
- New qPCR events in autumn 2009
- New qPCR workshop modules at the TATAA Biocenter Germany
=> http://tataa.gene-quantification.info/

European wide qPCR application workshops => register now !
=> course program spring - summer - autumn 2009
=> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pdf
=> http://www.gene-quantification.de/bioeps-courses-autumn-2009.pdf


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MIQE - media & press review

=> http://www.gene-quantification.de/miqe.html#press

International Scientists Secure Quality in Molecular Diagnostics

Consensus Guideline Reached For Quantitative Polymerase Chain Reaction
Press release by TATAA Biocenter

Internationale Wissenschaftler sorgen für Qualitätssicherung in der
Molekulardiagnostik

Real-timePCR data markup language

Letter of the MIQE authors
Letter to leading journals recommending the use of MIQE for quality control of
qPCR experiments.

IBT of the Academy of Sciences of the Czech Republic

qPCR Grows Up by genome web

qPCR Assay Quality assessment on SciTopics

Quest Agrees to Pay Fine for Misbranding Tests

Advancing DNA research safely and securely

What do you hope to achieve with the guidelines?

Data that Meets the MIQE Guidelines on Canadian BioTechnologist 2.0

GLOSSARY OF REAL-TIME PCR TERMS by M.Tevfik Dorak


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The MIQE Guidelines Minimum Information for Publication of Quantitative
Real-Time PCR Experiments
Stephen A. Bustin, Vladimir Benes, Jeremy A. Garson, Jan Hellemans, Jim Huggett,
Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory L.
Shipley, Jo Vandesompele, & Carl T. Wittwer
Clinical Chemistry 2009, 55(4): 611-622
=> http://miqe.gene-quantification.info
=> http://www.gene-quantification.de/miqe-bustin-et-al-clin-chem-2009.pdf

RDML: structured language and reporting guidelines for real-time quantitative
PCR data
Lefever S, Hellemans J, Pattyn F, Przybylski DR, Taylor C, Geurts R, Untergasser
A, Vandesompele J; on behalf of the RDML consortium. Center for Medical
Genetics, Ghent University Hospital, Ghent, Belgium.
Nucleic Acids Res. 2009 Apr;37(7): 2065-2069
=> http://rdml.gene-quantification.info
=> http://www.gene-quantification.de/lefever-et-al-rdml-2009.pdf

Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold
standard or substandard?
Murphy J, Bustin SA.
Expert Rev Mol Diagn. 2009 9(2):187-197
=>
http://www.gene-quantification.de/murphy-bustin-review-qpcr-optimization-2009.pd\
f

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA
expression and DNA copy number in multiple sclerosis.
Garson JA, Huggett JF, Bustin SA, Pfaffl MW, Benes V, Vandesompele J, Shipley
GL.
AIDS Res Hum Retroviruses. 2009 25(3): 377-378
=> http://www.gene-quantification.de/garson-et-al-aids-research-2009.pdf

Real-time polymerasechain reaction – towardsa more reliable, accurateand
relevant assay.
SA Bustin
EUROPEAN PHARMACEUTICAL REVIEW 2008 (6): 19-27
=> http://www.gene-quantification.de/bustin-review-qpcr-optimization-2009.pdf

In-House Nucleic Acid Amplification Assays in Research:
How Much Quality ControlIs Needed before One Can Rely upon the Results?
Petra Apfalter, UdoReischl and Margaret R. Hammerschlag
JOURNAL OF CLINICAL MICROBIOLOGY 2005 (dec): 5835–5841
=> http://www.gene-quantification.de/apfalter-review-qpcr-optimization-2005.pdf


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Upcoming Events World-wide academic and commercial qPCR Events
http://events.gene-quantification.info/

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars, qPCR
Education Program, etc.
Please submit your qPCR event here => events@...


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qPCR WORKSHOP

BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a 3-day qPCR
Core Module and a 2-day qPCR Biostatistics Module. All courses are held
regularly in Göteborg, Sweden, in English and in Freising-Weihenstephan,
Germany, in German and English, and in Prague, Czech Republic in English and
Czech.
Depending on the occasion the workshop language and the different prices may
apply. Further customized workshops and specialized trainings will be held as
well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in cooperation with BioEPS GmbH,
located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For more
information and registration, please see our web page:
=> http://TATAA.gene-quantification.info/


Course Occasions 2009:

- 3-day qPCR Core Module (Mon. - Wed.)
- 2-day BioStatistics Module (Thu. - Fri.)
- 3-day single-cell qPCR Module (Mon. - Wed.)
- 3-day microRNA Module (Mon. - Wed.)


13 - 15 July 2009 (E) NEW microRNA qPCR
27 - 31 July 2009 (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module (Thu. - Fri.)
14 - 16 September 2009 (E) NEW single-cell qPCR
19 - 23 September 2009 (E)
3-day microRNA Module (Mon. - Wed.) & 2-day BioStatistics (Thu. - Fri.)
26 - 28 October 2009 (E) 3-day qPCR Core Module (Mon. - Wed.)
16 - 20 November 2009 (E)
3-day microRNA Module (Mon. - Wed.) & 2-day BioStatistics (Thu. - Fri.)
7 - 11 December 2009 (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics (Thu. - Fri.)

=> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pdf
=> http://www.gene-quantification.de/bioeps-courses-autumn-2009.pdf


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Forward Please send the qPCR NEWS to further scientists and friends who are
interested in qPCR !


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.gene-quantification.info


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