Hello David,
 
We use Agilent's linear amplification labeling kit for yeast
(Saccharomyces,Candida and others) RNA labeling and it works great.  We have
seen this happening with aminoallyl labeling previously, since it is a chemical
reaction, PH salt or solvents might inhibit the reaction: Reprecipitation might
help.
 
Best wishes

Raja
=======================================
Dr Raja Mugasimangalam
CEO, Genotypic Technology [P] Ltd.,  www.genotypic.co.in
=============================================

--- On Tue, 7/7/09, Loose, David S <David.S.Loose@...> wrote:


From: Loose, David S <David.S.Loose@...>
Subject: yeast RNA
To: GENE-ARRAYS@...
Date: Tuesday, July 7, 2009, 9:25 PM








We have been having a real problem labeling yeast (C. albicans) RNA for the last
few weeks. The RNA looks fine by bioanalyzer but the single strand labeling kit
(Stratagene) is giving a low yield and a high background (aminoallyl labeling).
Is anyone having success with yeast microarrays?
 
David Loose, Ph.D.
Associate Professor
Integrative Biology & Pharmacology
Director, Microarray Core, and
   Graduate Program in Cell and Regulatory Biology
University of Texas Health Science Center at Houston
Houston, TX   77030
713-500-7440
 




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