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genotyping using real time PCR machine   Message List  
Reply | Forward Message #19773 of 19891 |
Hi there,
 
I have a question on Genotyping using real time PCR machine.
 
I heard Applied Biosystem offer Taqman SNP genotyping assay which uses VIC/FAM
label  probes. Bio-rad uses SsoFast EvaGreen supermix to differentiate Allele by
melting curve.
 
It is 1st time for us to work on Genotyping assay. Could you let me know if
there is other method to work on SNP via qPCR? and which method is better?
 
Thanks
 
Jean 



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Fri Jul 10, 2009 4:01 pm

jeanhofamily
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Message #19773 of 19891 |
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Hi there,   I have a question on Genotyping using real time PCR machine.   I heard Applied Biosystem offer Taqman SNP genotyping assay which uses VIC/FAM...
Jean ho
jeanhofamily
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Jul 13, 2009
7:44 am

Hello: I've forwarded your email to Sharon Norton (norton@...) who is responsible for the service information you requested. David _________ On...
Moraga Amador,David A
array_seq
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Jul 15, 2009
7:51 am

Hi Jean, There is a 'new to me' application called HRM (high resolution melting) available using an ABI 7500 fast instrument. Caprice S. Caprice Rosato...
Caprice Rosato
array_seq
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Jul 15, 2009
7:51 am

Hi Jean, I have been used Taqman SNP genotyping (VIC/FAM label probes) assays in the Stragene Mx3005P machine . I have excellent results, I think that this...
Cristina Junta
array_seq
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Jul 15, 2009
7:52 am

Dear Jean: Commercial response...Microarrays can perform genotyping very well, have you considered that as an option? There are many good papers describing a ...
Todd Martinsky
tmartinsky
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Jul 15, 2009
7:52 am

Hi Jean, Monte replied earlier to your post and he is absolutely right: This easiest solution is to to simply pick the assay from the AB website. What species...
edward.enright
edward.enright@...
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Jul 17, 2009
4:46 pm
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