Dear Sheetal Bhan, check this out, http://genomics.princeton.edu/dunham/ozone.html Ray _____________________________________________ You may leave the list at...
Ray Chen
rayccnthu@...
May 1, 2005 7:14 am
16959
hi, Ronald, I can not read the the words (0.5 to 5µl), would you please make it clear? regards, guozhen ... <GENE-ARRAYS@...> ... for UV/Vis...
guozhen Liu
gzhliu@...
May 1, 2005 7:14 am
16960
Dear all I have been trying to get RNA by laser capture for amplification and gene array. The amplification side I am happy with so far(I have not tested...
Nicholas Lea
nicholas.lea@...
May 1, 2005 7:16 am
16961
Dear Vanessa You can assess dye incorporation using any spectrophotometer. All you need is the Cy3 and Cy5 extinction coefficients then just do the ...
maria kamarinos
mariakamarinos@...
May 1, 2005 7:16 am
16962
Hello, Are you using indirect or direct labelling? We are experiencing similar problems when we use indirect labelling (on long oligo slides). With direct ...
Miroslava Cuperlovic-...
miroslavac@...
May 1, 2005 7:16 am
16963
hi Is there a way I can do alignments on multiple sequenes -say 100 genes all together. I want to determine if a final list of genes can be compared to find...
Sheetal Bhan
GENE-ARRAYS@...
May 1, 2005 7:16 am
16964
David I try to get rid of the wash solution 2 by dabbing on kimwipes. This didn't seem to help. And i use the wash solutions as recommended by agilent's...
Sheetal Bhan
GENE-ARRAYS@...
May 1, 2005 7:16 am
16965
All I have been using the Artus kit and I have amplified RNA from as little as 100pg of starting material (Yeild was 88ug). I get a good spread of molecular...
Nicholas Lea
nicholas.lea@...
May 1, 2005 7:16 am
16966
Keith, We are in the process of completing the AFX 10K training chips and we used the MJ PTC200 for our training and it worked fine. Based upon my...
Sandra Splinter BonDu...
GENE-ARRAYS@...
May 1, 2005 7:16 am
16967
Hi, As for the Dye Saver, I think you had left your slide in the Dye-Saver for too long. I had the same problem, but once I dipped my slide again in...
Ying, Jun
GENE-ARRAYS@...
May 1, 2005 7:16 am
16968
... Preferred candidates possess a Master's degree in basic or clinical sciences with a major emphasis in molecular biology, biochemistry, microbiology or a...
Mensink, Brenda M. (HR)
GENE-ARRAYS@...
May 1, 2005 7:16 am
16969
Dear Members I am a newbie in the field and recently came across staining molecules such as SybrGreen from Molecular Probes. Some of these Cyanin Dimers...
Maxime Rattier
maxime.rattier@...
May 1, 2005 7:16 am
16970
Dear sir or madom: I am working on rabbit mesenchymal cells .So I just want to know is there any rabbit micro array chip available ?If there is where can I...
farbod malek
farbodmalek@...
May 1, 2005 7:16 am
16971
Dear Microarrayers, We just purchased a large oligo library for spotting (the Illumina MEEBO set). Up until now we have only used cDNAs for spotting on to...
Dear Anita I agree with Nicolas and Lev. High level of humidity will restrict pin movement and there can be a problem in spotting. I have tried printing upto...
Dear All We bought AROS genome set from OPERON tech.oligos were dissolved in 3X SSC buffer. Operon recommends to store plate at -20 degrees C for max 2 week...
Sorry, do I get it right? Oligos degrading in 3xSSC at -20C after 2 weeks? I store my oligos (short ones, 18-28 nt) in 50% DMSO (spotting buffer) and in dH2O...
We have finalized our list of speakers for the Nortwest Gene Expression Conference. Please see below for a list of our speakers and their talks. We are also...
Kyle Serikawa
kyles@...
May 5, 2005 6:40 am
16976
Hi. Have you plotted your data in a log-ratio log-intensity plot? Where does the 5000 spots show up? If they show up at either low or high intensities, I am...
Henrik Bengtsson
hb@...
May 5, 2005 6:40 am
16977
You could use clustal x (clustal w is the same just a different interface) (general sequence alignment program) to be run from your own computer There are...
Sheryl Maher
s200530@...
May 5, 2005 6:40 am
16978
We are having high background -low signal with Cy3 dyes ...Is that just as sensistive to ozone as Cy5? another question is whether the background problerm...
Boyle, Susan
GENE-ARRAYS@...
May 5, 2005 6:40 am
16979
If you want use 5ul for OD, Shimadzu has a new unit called Bio Mini Spec may work for you. See detail at http://wanginfo.home.comcast.net/BLN/biominispec.htm ...
Martin J Wang
GENE-ARRAYS@...
May 5, 2005 6:40 am
16980
Hi, I am doing this comparison now, using both fold-change and analysis of variance to analyze the data. Found that there is considerable disagreement between...
Joseph Dubouzet
GENE-ARRAYS@...
May 5, 2005 6:40 am
16981
Hi.. TOUCAN is a tool for looking for several elements of TF...only or else you develop a java application with BioJava ti looking for a given consensus...
gregory voisin
voisingreg@...
May 5, 2005 6:41 am
16982
On a related note. Has anyone been successful in running DNA from Paraffin Embedded samples through the Affy SNP Protocol? If so, could you share your secrets?...
Monzon-Bordonaba, Fed...
GENE-ARRAYS@...
May 5, 2005 6:41 am
16983
Dear Microarrayers, We just purchased a large oligo library for spotting (the Illumina MEEBO set). Up until now we have only used cDNAs for spotting on to...
Dear Robert, The UltraGAPS slides will also work nicely for spotting oligonucleotides. Transitioning from cDNA to oligos should be relatively simple. There are...
Canel, Camilo
canelc@...
May 6, 2005 6:55 am
16985
Dear Bob I don't think you need to make any changes in your protocol if you shift from spotting cDNA to oligos. We use same protocol for PCR products and...
Hi, If you have a list of rabbit sequences, or could get it from a data base, the easiest way would be to order custom-made oligonucleotide chips. If you like...
smitmv
smitmv@...
May 6, 2005 6:56 am
16987
... Agilent CGH Analytics software training Come learn more about CGH Analytics, Agilent's java desktop software for CGH data mining and visualization. This is...
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