Hallow, Has any one tried to up concentrate RNA samples (dissolved in water) in speedovac? Salah [Non-text portions of this message have been removed]...
Salah Ibrahim
GENE-ARRAYS@...
Jun 1, 2005 7:00 am
17098
We use genetraffic with TIGRs TMEV software. Greg Greg Khitrov PhD Assistant Professor, Medicine Mount Sinai School of Medicine New York, NY 10029 (212)...
Greg Khitrov
greg.khitrov@...
Jun 1, 2005 7:01 am
17099
I wanted to let readers know about a new version of our free software for gene expression analysis in the context of functional categories (e.g., Gene...
Paul Pavlidis
GENE-ARRAYS@...
Jun 1, 2005 7:01 am
17100
Dear colleagues, I am pleased to send you the announcement of the course on microarray data analysis that my collaborators and I are organizing for next July...
I have a custom array and use GeneSpring for data analysis. Joy Bruno Senior Environmental Toxicologist Pacific Environmental Science Centre 2645 Dollarton...
Bruno,Joy [PYR]
joy.bruno@...
Jun 1, 2005 7:01 am
17102
We use GCOS and Bioconductor for the probe level data and GeneSpring, R and Pathport Toolbus for analysis. I have not yet found an annotation software that I...
hannacraig
hannacraig@...
Jun 1, 2005 7:01 am
17103
You might want to try out avadis. It is a neat piece of software by Strand Genomics and is pretty good for analyzing Affy data. ...
sowmyanarayan sriniva...
GENE-ARRAYS@...
Jun 1, 2005 7:01 am
17104
Giles I have used Axon 4000B for several years. Know nothing about Biorad Versarray. Axon uses dried slide, single slide loading and scaning. We had two...
We use GCOS and Bioconductor for the probe level data and GeneSpring, R and Pathport Toolbus for analysis. I have not yet found an annotation software that I...
hannacraig
hannacraig@...
Jun 1, 2005 7:02 am
17106
Hello, Is there an archive or an alternative front end of this site. When I use the groups.yahoo.com search function, I can only search a few dozen messages...
Lingsheng, Visit www.bioconductor.org. There is a package you can do exactly that. Good luck! Martin ... Martin Wang BLN West Coast Tel: 650-638-1758 Fax:...
I am working with whole fish (salmonids, approx. 1-1.5 gms). The fish were frozen and homogenized in Liquid Nitrogen. Total RNA has been extracted using...
Sally
sagoldes@...
Jun 2, 2005 7:30 am
17110
I am having a tremendous problem getting my samples to label with the Cy-3 Dye. The Cy-5's seem to be working completely fine. The other thing is that it is...
We do it all the time with very good results. We keep our SpeedyVac rotor, lid and bowl wiped down with peroxide on a regular basis. Make sure that it is well...
Dennis Burian
GENE-ARRAYS@...
Jun 2, 2005 7:30 am
17112
We use Partek for the statistic analysis. It is very easy to use, especially for biologist like us. Partek Pro could handle a very large data set, which is its...
Yang, Jun (NIH/NIAID)
JYang@...
Jun 2, 2005 7:30 am
17113
The type of gene set analysis described by Dr. Pavlidis can be much more powerful than analyzing individual genes for differential expression and then post-hoc...
Simon, Richard (NIH/N...
RSimon@...
Jun 2, 2005 7:30 am
17114
yes - it is easy, provided there are no protein contaminants in the sample, which would contain ribonucleases. also don't let the RNA dry out completely. matt ...
In line with the question posted recently, could I ask what software people are using for analysis of array CGH type experiments? I am currently evaluating...
Joo Wook Ahn
GENE-ARRAYS@...
Jun 2, 2005 7:31 am
17116
The next two-day microarray data analysis workshop presented by Dr. Sorin Draghici, Head of the Joint Bioinformatics Core serving Wayne State University’s...
Alexander Kuklin
kuklina@...
Jun 2, 2005 7:31 am
17118
The type of gene set analysis described by Dr. Pavlidis can be much more powerful than analyzing individual genes for differential expression and then post-hoc...
Simon, Richard (NIH/N...
RSimon@...
Jun 2, 2005 7:31 am
17119
Hello Salah! I have successfully done this. Set your speedvac to run at room temperature, and all seems to go o.k. Joe Andrews Robarts Research Institute ... ...
Joseph Andrews
jandrews@...
Jun 2, 2005 7:31 am
17120
hey Salah I have concentrated total RNA samples dissolved in water in a speed vac and it works perfectly well. I don't see any degradation and there is no loss...
Sheetal Bhan
GENE-ARRAYS@...
Jun 2, 2005 7:33 am
17121
salah depends on the source ... for cRNA from IVT you can concentrate it to powder if you have extracted with organic ... for total RNA from cultured cells its...
Rudy Spangler
spanglr@...
Jun 2, 2005 7:33 am
17122
Dear Rob, In general, the potential advantage of direct comparison designs that do not use a common reference is that all of the specimens hybridized to the...
Simon, Richard (NIH/N...
RSimon@...
Jun 2, 2005 7:34 am
17123
hi i use TIGR MIDAS & TMEV ! best sabari. _____________________________________________ You may leave the list at any time by sending a message- "SIGNOFF...
Sabari Thirupathy
GENE-ARRAYS@...
Jun 2, 2005 7:34 am
17124
Dr. Srinivasan, Please excuse the blatant commercialization of this post, but I think there is another product you might want to take a look at: GeneSifter....
Hi, we use a speedvac for concentrating RNA.The yield was about 30 % comparing before and after dissolving the speedvac dried RNA. 1)We made a calibration line...
Hi I routeinly concentrate the RNA using speedvac , for total RNA it is not a good idea to completely dry the total RNA solution. It is very hard to...
nila_200
nila_200@...
Jun 2, 2005 3:59 pm
17127
Hi Buddies, I am trying to use SVM to separate my microarray samples by a set of genes. I found SVM method was described in "Support Vector machine ...
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