In Genisphere's recent newsletter issue, there is a short article on recommendations to prevent Cy5/Alexa 647/Oyster-650 fading: ...
Joan Ferola
Joan_Ferola@...
Jul 1, 2005 8:31 am
17306
Hello Jakob, We had the exact same problem with our ScanArray Express HT that you describe here, except that our Cy5 signal loss was more pronounced and we...
Dear All We to have a scanarray express HT scanner from perkin elmer and are sufffering identical problems at the moment. We can scan an idvidual slide at a...
Implen expands services to Switzerland Munich, Germany, July 1, 2005 Implen, a Munich based leading supplier of instruments and consumables for microarray...
Are there any studies which compare efficieny (time, accuracy etc.) of different methods and software of probe/oligo designing? Any comments would be helpful. ...
Hi Harish We found the following tool quite useful for our oligo design. www.cbs.dtu.dk/services/OligoWiz/. Nucleic Acids Research, 2003, Vol. 31, No. 13...
Jennifer Chaseley
chaseley@...
Jul 1, 2005 7:51 pm
17311
Yannick, 25 years ago we had the sam problem with photo resist sticking to a silicon wafer. Humidity or ozone affected the wafer resist surface with time and...
Dear Jennifer, Bleaching depends on excitation energy you bring to a local area (scan time, laser power, resolution/beam diameter). Repetitive scans bring more...
rabe1au
rabe1au@...
Jul 4, 2005 7:57 am
17314
Hello, everyone, I did four slides last month, about 60% were black holes. For Cy3 and cy5 labeld cDNA, I mixed ~30pmol for each of them and applied on slide...
Clearly your slide surface takes much more dyes. Try blocking before hybridization. Good luck! Martin ... Martin Wang WIS Biomed Tel: 650-638-1758 Fax:...
be more careful washing pre- and post-hyb and dry quickly after washing. the holes are usually high background fluorescence. ... and cy5 labeld cDNA, I mixed...
dear all, iam a new member in the group iam interesting with microarray i have the reselts of bacterial microarrarray experiment but i don,t know how to...
what is/are the source(s) of the appearance of many spots displaying differential expression (i.e., total red or green staining) when only a few spots should...
Has anyone used peripheral blood mononuclear cells from disease state and normal patients to compare gene expression data? I got an email from a guy that said...
Hi. arrayer We have a GenIII spotter. It is working well. The machine is not produced right now. We can't buy GenIII spotter and its major components from...
It has been done with success in cows. See Paul Coussens' paper, 'Rapid and transient activation of gene expression in peripheral blood mononuclear cells from...
Hello All, I was just wondering if anybody can give me some idea/cite some publications about, how to get 'good' intensity cut offs. Quick elaboration of the...
Prasad Phirke
GENE-ARRAYS@...
Jul 7, 2005 8:07 pm
17324
Hey All, I wonder if somebody from the array group can provide me with references and good review articles about Microarray Applications into Diagnostics...
Ana Arnizaut
anarkizo88@...
Jul 7, 2005 8:08 pm
17325
I think it is better to use a simple t-test on the genes after filtering by A/P call only, and not after filtering by fold-change. There is no a priori ...
Howard Edenberg
edenberg@...
Jul 7, 2005 8:08 pm
17326
Even though the "R" in RMA stands for robust, I would ignore the arrays I suspect are degraded or bad in some way if I have sufficient number of arrays...
Adaikalavan Ramasamy
ramasamy@...
Jul 7, 2005 8:17 pm
17327
Hi there, Has anyone done any comparisons of GenePix 5.0 and GenePix 6.0? Thanks, Aubree...
On Behalf of: Ameeta Agarwal, Ph.D. Research Scientist National Center for Natural Products Research University of Mississippi University, MS 38677 Phone:...
Chandi Griffin
aniya@...
Jul 7, 2005 8:20 pm
17329
Are your custom cdf files available? Federico ... From: Share info on GeneChips tech and Microarrays in general [mailto:GENE-ARRAYS@...]On Behalf...
Monzon-Bordonaba, Fed...
GENE-ARRAYS@...
Jul 7, 2005 8:42 pm
17330
Hi, Questions regarding various tools and approaches to help with the biological interpretation of microarray results seem to appear periodically on this list....
Sorin Draghici
sorin@...
Jul 7, 2005 9:02 pm
17331
Hello; 1. I am using GeneSpring, and when I use ANOVA (in a time series analysis), and try to use FDR, even with a p value of 0.1, no gene appears significant,...
Hello, We are evaluating oligos for printing on Ultra-GAPs slides. Does anybody have any comments on the Illumina brand (MEEBO for mouse arrays). Are they...
Hello fellow microarrayers, I am at the University of Wisconsin, where we are closing down a lab and need to liquidate some of the larger equipment. None of...
Hi Everyone, We have a new Field Application Engineer position with BioDiscovery. Please see the description below. Job Description: The Field Application...
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