Hi Kirsten, According to our experience it is not necessary to work in the dark with cyanine fluorophors; just avoid long exposure to daylight and of course...
Kirsten, Use foil to cover the tubes should be good enough. You can also keep the reaction tubes in a drawer to avoid light. There are other dyes available,...
Hi all! We have been having problems with large spot size after speed vac'ing plates. Has any one had this happen, or have suggestions on how to avoid this...
janicepfeiff
janicepfeiff@...
Aug 2, 2005 4:57 pm
17426
Dear Dr. Whitehek, SCHOTT Nexterion has developed a flat bottom microtiterplate kit that includes a 96-well plate. The plate is available coated in a variety...
Dear All, We have just released rptdb (www.jicgenomelab.co.uk/rptdb) specifically to allow users to get average report file QC data and to get pass/fail...
Janice, I may be ableto help. Whose plates are you using? Bill Moffat janicepfeiff <janicepfeiff@...> wrote: Hi all! We have been having problems with...
We want to monitor whole genome expression after induction of a set of genes on a plasmid. Is it more appropriate to perform a time series and compare various...
Hello Janice, What type of plates are you drying down your clones in? Are you using a radiant cover feature on your speedvac, or doing a cool dry-down? What...
Dear all, does anybody know an example of a gene ( including literature) that is induced at low dose and repressed at high dose in immune cells...
steppan1971
steppan1971@...
Aug 4, 2005 5:41 am
17435
Hi again! Does anyone have a protocol for Cot1 DNA synthesis for blocking our arrays? The only commercially available ones I can find are from human & mouse, &...
Hi all, Was wondering generally how much of cy-labelled cDNA target (pmol or ng amounts) do you add to your cDNA microarrays? Do you equalise the pmol amounts...
Dear Jeff, Please do check out NetPro from Molecular Connections. It Provides you with pathways, interactions, drugs, mutations, kinetics, transgenics and...
Dear All, In a dual color microrray experiment the target input should be an equal amount of nucleic acid or an equal amount of label? I think it makes a ...
Ferenc Marincs
F.Marincs@...
Aug 4, 2005 5:41 am
17439
Dear All, In a dual color microrray experiment the target input should be an equal amount of nucleic acid or an equal amount of label? I think it makes a ...
Ferenc Marincs
F.Marincs@...
Aug 4, 2005 5:41 am
17440
GenMAPP News: GenMAPP is now available for dog. A Gene Database and MAPP Archives for C.familiaris are available for download via GenMAPP, under Data>Download...
GenMAPP
GENE-ARRAYS@...
Aug 4, 2005 5:41 am
17441
What are the favourite methods for desalting synthetic DNA oligonucleotides on a 96-plate scale? Our oligo's are made by the phosphoramidite method. We have...
Adrian Culf
GENE-ARRAYS@...
Aug 4, 2005 5:41 am
17442
Greetings: We are currently analyzing a few different strains of Staphylococcus using customer-made arrays. Our problem is the first step to break up the...
W_Fan
w_fan@...
Aug 4, 2005 5:42 am
17443
... This is the reference I usually use for K means clustering. I hope you find it useful. Tavazoie S, et al. Systematic determination of genetic network...
I'm interviewing professional life scientists for a market research project. Specifically, I am interested in the attitudes and product preferences (including...
sciencemarketer
sciencemarketer@...
Aug 4, 2005 6:08 pm
17446
Hi all, i our lab we are using sonication as the mechanical technique for the cell lysis.. we have been lysing bacterial cells through sonication in the...
Hello and thanks for the responces. We are spotting oligos on epoxy slides. It is only post speed vac of our plates that we are having problems with large spot...
excuse me, I look for the reference of Eisen 1998 PNAS, but no result on the website Have you the exact reference ? thanks a lot, Hervé Lucy McWilliam...
Dear Ferenc, Please share any data if you have with the group. In theory, any change of DNA concentration (given fixed volume in same hybridization) will...
Martin J Wang
GENE-ARRAYS@...
Aug 5, 2005 4:54 am
17450
Rajini, Assume the samples purified (so not much free dyes in the samples), use 30~60 pmol of each target. Martin ... Martin Wang WIS Biomed Tel: 650-638-1758 ...
Hi all, The IIT Research Institute is looking for two experienced Molecular Biologists. Scroll down to read the job descriptions for a SENIOR MOLECULAR...
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