Hi I am wondering if anyone has used the In Vitrogen BioPrime Plus labeling method for DNA to label target for Affymetrix arrays? Sally ...
Sally Ann Leong
sal@...
Jan 4, 2006 8:30 am
17924
Dear Sally, I am using the Invitrogen's bioprime indirect labeling method for DNA, not for affy! but works fine with my custom made arrays. good luck _sankar. ...
Sabari Thirupathy
GENE-ARRAYS@...
Jan 5, 2006 8:36 am
17925
BMN PRESS RELEASE Circulation Dept BMN 714-434-9500 phone info@... LAST CHANCE TO ORDER TOP 750 NANOTECH BIOTECH/BIOMED MANUFACTURERS...
E-mailNewsService
biomednews1@...
Jan 5, 2006 8:37 am
17926
We use this kit, but not for Affy arrays. We have optimized the protocols a bit for our application. We found that it is the best way to label to get the most...
Nick Holtan
GENE-ARRAYS@...
Jan 5, 2006 8:37 am
17927
Hi All, We have an Affymetrix 428 scanner which has not gotten much use recently. I have been getting queries lately regarding scanning some Agilent arrays as...
Spatrick, Phyllis
GENE-ARRAYS@...
Jan 7, 2006 6:53 pm
17928
Greetings, I have a small problem that is aggravated by my own ignorance, and I was hoping to get a point in the right direction from a more experienced...
Nick I assume that your are making custom 60-70 mer arrays? Have you tried to make smaller target products as described at www.naturalvariation.org? I guess...
Sally Leong
sal@...
Jan 7, 2006 6:53 pm
17930
I know this is a contentious issue from browsing the archives ... but could anyone please comment on the validity of using a reference sample consisting of a...
Paul Lang
paul.lang@...
Jan 7, 2006 6:54 pm
17931
Dear Colleague, This is a final reminder of the upcoming "Genomes to Systems" Conference to be held in the Manchester (UK) International Convention Centre,...
arcossins
cossins@...
Jan 9, 2006 8:15 am
17932
Hello, I am looking for a company or facility to print slides. Although I have been ordering printing to a printing facility and am satisfied with the...
Dr.Sato, Dr.Patricia Soteropoulos of CAG, NJIT can tell you more. She is the director of the core facility in Newark,NJ. bioinformaticist. Masanao Sato...
Hello List members, A local company has one HP G2500A GeneArray scanner, two Affy fluidics station (model 400) for sale. Email me if you are interested to...
Martin Wang
GENE-ARRAYS@...
Jan 10, 2006 8:19 am
17935
Hi, Is anyone know how change extinction coef. And quantum yield of Cy3 and Cy5 when are attached to DNA (Thimidine)? Can you give me some data about Cy-DNA:...
Loman, A.
GENE-ARRAYS@...
Jan 10, 2006 8:19 am
17936
Hello, In the past, I used Affy 428 scanner + Jaguar. However, we had to buy another scanner (Axon) because the minimum resolution was 20 µm and we needed 10...
Atienzar Franck
Franck.Atienzar@...
Jan 10, 2006 8:19 am
17937
Hello, You might try dmitry.trifonov@... who has installed his Exact and Thomas array printers in large printing facilities. Each compact printer ...
Hi, Is anyone know how change extinction coef. And quantum yield of Cy3 and Cy5 when are attached to DNA (Thimidine)? Can you give me some data about Cy-DNA:...
Loman, A.
GENE-ARRAYS@...
Jan 10, 2006 8:20 am
17939
Are there any core facilities on this list who are currently printing protein custom arrays ? Thanks, and Happy New Year. Caprice . . . . . . . . . . . . . . ....
Caprice Rosato
GENE-ARRAYS@...
Jan 10, 2006 8:20 am
17940
Hi Masano, You may want to look at Microarray Inc. (http://www.microarrays.com/). We are very pleased with their print quailty and price for our custom 30K+...
Dear Masano You could look at Ocimum Biosolutions (www.ocimumbio.com). They have a host of arrays (catalog, custom, starter etc.) and a good printing division...
Rekha.M
rekha.m@...
Jan 10, 2006 3:11 pm
17942
Hi Masanao KamTek Inc, offers Microarray services in Genomics, Proteomics and Glycomics. We have our clients in NIH, FDA, USAMRIID, state Universities and...
Hi Caprice KamTek Inc, has state of art microarray printing facility at Gaithersburg, Maryland. We offer services in Ganomics, Proteomics and Glycomics to NIH,...
Dear George, If I understand your question correctly, GeneSpring should provide you the answers you seek. I've been working with the program for 2 years. The...
Seth Crosby
springerrr@...
Jan 10, 2006 10:54 pm
17945
Dear Phyllis, There is a more user-friendly package available that is compatible with the Affymetrix 428 scanner and that is BlueFuse for Microarrays. We...
David Chrimes
david.chrimes@...
Jan 11, 2006 9:13 am
17946
HI I follow an indirect labeling protocol, and have been doing quite well when I take 100 picomol of labelled reaction from each dye, Cy 3 and Cy 5, and...
Hello Phyllis, I just thought I would toss my "two cents" in here regarding the BlueFuse software. I've seen the program in action and can say that I am very...
We do, for example. We use a piezo spotter (sciflexarrayer) - works fine with proteins (other probes as well: antibodies, peptides, carbohydrates etc). Jens ...
Jens Sobek
GENE-ARRAYS@...
Jan 12, 2006 8:22 am
17950
Hello All, A very happy new year and hope everybody is happy to be back in office with new resolutions. I have performed few microarray experiments with...
Prasad Phirke
GENE-ARRAYS@...
Jan 12, 2006 8:23 am
17951
Hi Paul, If you are going to do time series experiments, it is recommendable to use a reference design over the loop design. You can read Storey's studies, he,...
Mauricio Rodriguez
mrodriguez_lanetty@...
Jan 12, 2006 8:23 am
17952
Hi I would like to update the annotation of my custom-made agilent RAT microarray. A tool such as probelynx (http://koch.pathogenomics.ca/probelynx/) can...
floor stam
GENE-ARRAYS@...
Jan 12, 2006 8:24 am
17953
Hi Anastasia Here are some formulae that might help you, including the extinction coefficients for the dyes cy3 and cy5. Yield of cDNA (ng) = A260 X 37 ng/µl...
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