Dear Arno, we have made extensive experiments to address this question. Last year we were testing CY3-related cyanine dyes (CY3, Alexa555, some dyes from ...
Jens Sobek
GENE-ARRAYS@...
Feb 1, 2006 8:51 am
18031
Dear Matthew, In the past, we used cDNA arrays and we had the choice to work either with Cy3 or Cy5. We tried both dyes and we had better results with Cy5. Now...
Atienzar Franck
Franck.Atienzar@...
Feb 1, 2006 8:53 am
18032
From: "Chris Barker" <cbarker@...> To: "'Share info on GeneChips tech and Microarrays in general'" <GENE-ARRAYS@...> Subject: RE:...
Chandi Griffin
aniya@...
Feb 1, 2006 8:55 am
18033
Generator Microsoft Word 11 (filtered medium) Dear Colleagues, Scientific Services at The Jackson Laboratory (http://www.jax.org) is conducting benchmarking...
Sonya Kamdar
GENE-ARRAYS@...
Feb 1, 2006 1:24 pm
18034
Dear Matt: "Staining" can definitely be performed on spotted microarrays, PE can be picked up in Cy3 channel. Many binding and washing steps are routinely ...
Todd Martinsky
todd@...
Feb 1, 2006 1:25 pm
18035
Post-Doctoral Positions in Bioinformatics, Genomics and Proteomics (through the Natural Sciences and Engineering Research Council of Canada Visiting ...
Paul White
paul_white@...
Feb 1, 2006 1:26 pm
18036
Hi all, I am new to the group and I want to know what microarray is all about in a paragraph with reference web sites. can anyone help me? TX Carole Yauk...
Hello Matt Developed in cooperation with the Brown lab at Stanford, SciGene recently introduced the BriteSpot Microarray Workstation for hybridizing, washing...
Jim Stanchfield
jstanchfield@...
Feb 3, 2006 8:40 am
18041
I can not speak about who first used a 'housekeeping' gene but 9 years again when we started with a qPCR lab here the rational was to normalize against an...
Nancy Shipley
Nancy.S.Shipley@...
Feb 3, 2006 8:40 am
18042
Miltenyi Biotec Technical Seminar Series You are invited to attend this informative seminar "Enhanced speed and reproducibility of microarray applications ...
Hello All, First of all, excuse me for posting few of the queries again but I need bit more/specific clarification. I am basically chemist and not good at...
Jiang Miao
miaoj5@...
Feb 4, 2006 2:22 pm
18044
Dear colleagues, I would be very grateful if someone gives me suggestions about the interpretation of the RIN value (RNA Integrity Number) obtained with the ...
Silvia Pellegrini
GENE-ARRAYS@...
Feb 4, 2006 2:22 pm
18045
Jiang et al., A good start for getting responses to your questions: "Data Analysis Tools for DNA Microarray"; Editor= "Chapman & Hall/CRC"; Author="Sorin...
Duval, Manuel
GENE-ARRAYS@...
Feb 4, 2006 2:23 pm
18046
Hi folks, does any of you use ImaGene and GeneSight (from biodiscovery) regards H canatan Jiang Miao <miaoj5@...> wrote: Hello All, First of all,...
... You can use the report (.RPT) file from affymetrix in order to have some basic information about control probes, raw intensities and so on. Nevertheless...
Remo Sanges
sanges@...
Feb 7, 2006 8:26 am
18048
Dear Jiang, I would like to suggest GeneSpring. www.genespring.com Download Genespring GX and get the fully functional demo license. All the steps are clearly...
Raja Mugasimangalam
GENE-ARRAYS@...
Feb 7, 2006 8:26 am
18049
Hi Jiang, I agree with Manuel, Sorin has the best, most lucidly written text for handling your problems and I'd strongly recommend you get a copy if you are...
Eric Blalock
GENE-ARRAYS@...
Feb 7, 2006 8:27 am
18050
Hello: We are looking for a used microarrayer. I'd appreciate if you can keep me informed when such a unit becomes available. Sincerely, Mingdi -- Mingdi Yan,...
Yan Mingdi
yanm@...
Feb 7, 2006 8:29 am
18051
Hello all, I'm currently having a problem with my manual hybridisation method. I'm using the single corning hybridisation chambers (#2551)and Ambion's...
Hai all.. I am new to Microarray data analysis and have some confusion regarding using the existing data sets. I have confusion regarding the Golub et al data...
Aneesh Baby
aneeshbaby@...
Feb 8, 2006 7:39 am
18053
Dr. Yan (and others with similar needs...), We may be able to help with your search for a used microarrayer. We have the following 5 pieces of equipment to...
We have just purchased the Affymetrix system - I noticed that several companies provide kits for labeling RNA (Ambion, ENZO and Affy). I was wondering what...
Whistler, Toni
taw6@...
Feb 8, 2006 7:41 am
18055
Dear Jonathan, Want you see are gradients caused by large cover slips. I assume that you use with 40 µl hybridisation volume cover slips greater then e.g....
Hi Mingdi, I've got 2 Molecular Dynamics GenIII Arrayers for sale. If you are interested I can send you more info. Thanks Scott William "Scott" Hutto ...
Dear Jonathan, We can help you. The answer to your problems is our new development of DiscoverSlips and the handling tool PALMhyb I for one glass slide. A...
Hi, I wonder if there is anyone in this group who works on DNA probe design for microarrays (cDNA, universal, ...). I started working on this problem about a...
I suppose it depends on what you call a 'gene'. the number probably more accurately refers to 'transcripts'. this will include 'known' genes, 'unknown' genes,...
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