Hello, I have a problem that I cannot imagine is unique in microarray analysis, yet I have found surprisingly little help online on ways to solve it. I am...
heteroduplex
kartik.pappu@...
Apr 3, 2006 7:04 am
18196
Have a look at Tetrahedron Letters Vol 22, No 34, pp3265 - 3268, 1981 They describe the ring opening of Thymine and Thymidin under UV and subsequent addition...
more than 1440 papers have been collected at the online bibliography of microarray data analysis http://www.nslij-genetics.org/microarray/ the goal is to have...
wli625
wli@...
Apr 3, 2006 3:57 pm
18198
Dear List Friends, I am starting to hybridizing my slides and I am get bad images: a lot of background with a few spots. I am suppose that my RNA is bad. I...
Marcelo Luiz de Laia
mlaia@...
Apr 4, 2006 7:06 am
18199
hi this is mukesh, student of master of statistics final semester & i've been searching contents of micro-array along with some contents regarding how...
mukesh_chatt
mukesh_chatt@...
Apr 4, 2006 7:06 am
18200
Dear arrayers, Does anyone aware of bovine microarray, either oligo or cDNA, that we could purchase? Many thanks for your help. Frank ...
Marincs Ferenc
GENE-ARRAYS@...
Apr 4, 2006 7:07 am
18201
Hi, We have processed the Affymetrix Bovine arrays for a customer recently. They looked very good. The cost was 580 GBP per sample. We are an Affymetrix...
Whenever I try to hydrolyze/neutralize after an RT, all of my cDNA is degraded (<<100 bp). I use the standard alkaline methods: NaOH/EDTA and Tris - or -...
Hi Debby, I think you're main problem will be that the Tm of a 4-mer is lower than room temperature. This was also the problem with the 9-mer hyb protocol...
Chris Seidel
GENE-ARRAYS@...
Apr 5, 2006 7:13 am
18204
Hello Everyone, Just a quick note regarding the email address to which any problems regarding the Gene-Arrays list should be sent. Effective immediately, all...
Chandi Griffin
GENE-ARRAYS@...
Apr 5, 2006 7:13 am
18205
Hi Frank Affymetrix has a bovine GeneChip Phyllis ... From: Share info on GeneChips tech and Microarrays in general [mailto:GENE-ARRAYS@...] On...
Spatrick, Phyllis
GENE-ARRAYS@...
Apr 5, 2006 7:14 am
18206
Affymetrix makes a bovine array: http://www.affymetrix.com/products/arrays/specific/bovine.affx The GeneChip(r) Bovine Genome Array can be used to study gene ...
Bailey, Dione
GENE-ARRAYS@...
Apr 5, 2006 7:14 am
18207
Dear Frank/Gene-arrays users Affymetrix has released Bovine genome array For more information, please review the data sheet ...
Dilair Baban
GENE-ARRAYS@...
Apr 5, 2006 7:14 am
18208
Hello array people, Are there any oligo based expression arrays, commercial or otherwise, that look at both sense and antisense on the same array? Mouse or...
Becker, Kevin (NIH/NI...
beckerk@...
Apr 5, 2006 7:14 am
18209
Dr. Marincs: We have 4600 bovine genes arrayed on a glass slide for $250. This represents our cost of production. the array was produced from libraries of...
Bob Collier
rcollier@...
Apr 5, 2006 7:15 am
18210
... Looks like tRNA to me, down at the bottom of the size range. Your prep looks to be of good quality since the bands are sharp and clear. However in order...
Peter Ellis
pjie2@...
Apr 5, 2006 7:15 am
18211
Hi Marcelo-- Looking at your picture, it seems that in the right hand panel, you have very little of the highest molecular weight band, and a lot of the lowest...
Nicole Donofrio
ndonofr@...
Apr 5, 2006 7:15 am
18212
... Dear Marcelo, your RNA looks beautiful. The top band is DNA, as you suspect. If you extract with Trizol, try not to be too "greedy" and get all of the...
Jose de_las_Heras
j.delasheras@...
Apr 5, 2006 7:15 am
18213
... Contact Jeanne Burton at Michigan State as they sell an 18k element cDNA array and a long oligo array. Here is her contact information: Jeanne L. Burton,...
David Henderson
GENE-ARRAYS@...
Apr 5, 2006 7:15 am
18214
Hello Marcelo, The gel does suggest some gDNA contamination (top band). This is a fairly normal level of contamination for a Trizol type prep. The unknown...
Penn Whitley
GENE-ARRAYS@...
Apr 5, 2006 7:16 am
18215
Dr Marincs, We can provide you high quality 60-mer agilent arrays ( 2x 11K arrays printed on one microarray slide) at very attractive price. Best wishes Raja ...
All Affymetrix SNP (Mapping) chips does, e.g. 10K, 100K and 500K (except for a tiny fraction of SNPs). /Henrik PS. Could the list maintainer please update to a...
Henrik Bengtsson
hb@...
Apr 6, 2006 7:54 am
18217
Dear Mukesh, Take a look at the Technical Reports section in my website http://linus.nci.nih.gov/brb It contains many reprints and presentations that will be...
Simon, Richard (NIH/N...
GENE-ARRAYS@...
Apr 6, 2006 7:57 am
18218
Our recent microarray experiments picked up several genes related to DNA methylation and epigenetic regulation that were up-regulated in our data set. We are...
Ciprian Gheorghe
cgheorghe@...
Apr 7, 2006 7:23 am
18219
Does anyone know of a source of hamster cDNA or oligonucleotide microarrays? Also, does anyone have any experience using mouse gene microarrays to analyze...
What are users impressions of comparisons between long oligos printing with or without amine linkers. We have seen nice results with both systems, but has...
Mark Band
markband@...
Apr 11, 2006 7:14 am
18222
Mark, Additional thoughts on amino linkers for oligo printing. In the the 1960 to 1980 period the integrated circuit industry had the same problem. they...
Hi Mark: Most of the users I speak to who are using long oligos are not using linkers and cross linking. Everything costs more when you're using linkers, both...
Todd Martinsky
todd@...
Apr 12, 2006 4:02 pm
18224
Hi Mark, We make a lot of experiment to identify the best way to print oligos (specially 70 mer). When we printed 70 mer with amino-modifier (C6) on epoxy...
Hi all, I am in the process of confirming my microarray data from lasermicrodissected neurons (2-round RNA amplification/Affymetrix) with real-time RT-PCR from...
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