Dear all, if you're worried about printing errors, why not considering non-contact dispensing? Our Nano-Plotter checks the function of each individual pipet...
Frank-Ulrich Gast
GENE-ARRAYS@...
Jun 1, 2006 8:05 am
18367
Jiang, I think one very important thing to keep in mind is that, if the clustering results are going to be of any analytical use to you, you must at least have...
Taboada, Eduardo
GENE-ARRAYS@...
Jun 1, 2006 8:07 am
18368
Hi everyone, Someone asked me an interesting question: is the Affymetrix array reusable? I have been told by Affy that their array is non-reusable. But I...
Kaiman Peng
kaiman.peng@...
Jun 1, 2006 8:09 am
18369
Hi Sidek, There is a paper comparing Affy with llumina: Barnes M., et al, Experimental comparison..., NAR, 2005, p.5914-23. Good luck, Oleg Suslov University...
... Do you use coverslips, lifterslips, other? if lifterslips, which end do you load the array from? The bias could be from the hyb i.e. lack of mixing, or...
Aaron Jeffs
GENE-ARRAYS@...
Jun 1, 2006 8:11 am
18371
Hi all, I am working with a Packard Piezoarray printer to print protein microarrays. It works well, except for issues with tip clogging. I haven't been able...
Hi Mike, It is strange that a dye-bleaching problem would differentially affect the first few rows in each block... We have seen similar issues which have...
Dear Sidek, what are you looking at SNP genotyping or expression.Both counts Illumina is way ahead wrt to content,redundancy and the cost per sample.. Cheers ...
Dear Craig, have you found a source for the rings and pins ? we can help you if you still need support we have currently 2 used GMS417 on hand please feel free...
Dear All, we have two 2nd hand Affymetrix GMS417 Arrayers to sell. Both instruments are in well condition and have been in use for some time - recalibration...
If mikes suggestion that it may be a time/humidity problem. The newer slides are being produced with a surface that is impervious to time/humidity problems....
Dear Jakob: We just put a bunch a group of slides together, that seems to work. Another alternative is to use Gel Plate Glass a common size would be 80mm x ...
Todd Martinsky
todd@...
Jun 2, 2006 9:18 am
18379
Hi Jakob, If you are having issues with spot uniformity due to excess liquid sheeting off the outside of the pins (prespotting), you might be interested in...
I agree with Aaron that lack of mixing during hybridization could be the problem. LifterSlip may be better than the conventional cover slip, but I also...
GENE-ARRAYS@...
Jun 2, 2006 9:20 am
18381
Dear Mike, I understand you are having a gradient problem across your array. According to what you posted, if you look at one channel at a time of your image,...
Sandra Lew
GENE-ARRAYS@...
Jun 2, 2006 9:20 am
18382
I agree with Aaron. This is likely the scanner. A quick check that we often do when this occurs is to flip the slide around to scan from "bottom" to "top"...
Darrell Ralph Boverhof
boverho5@...
Jun 2, 2006 9:20 am
18383
I agree with Aaron. This is likely the scanner. A quick check that we often do when this occurs is to flip the slide around to scan from "bottom" to "top"...
Darrell Ralph Boverhof
boverho5@...
Jun 2, 2006 9:20 am
18384
Yes, do the "180 degree test". Scan you microarray, then turn it around and scan it again. If you see the same top to bottom problem on both scans, then the...
Todd Martinsky
todd@...
Jun 2, 2006 9:22 am
18385
Thanks Bart for your response. ********************************************************************** Dr. Mark Band Director of Functional Genomics The W.M....
Mark Band
markband@...
Jun 2, 2006 9:24 am
18386
Probably a long shot... but in the past I've observed fluorescence contamination (always on teh Cy3 channel) coming from a wide range of sources. Where on the...
Jose de_las_Heras
j.delasheras@...
Jun 4, 2006 2:30 pm
18387
Dear Michael, There are multiple possible causes of the problem you describe. One is the possibility of the arrays being exposed to light or other signal...
Camilo Canel, T-US
GENE-ARRAYS@...
Jun 4, 2006 2:31 pm
18388
DNAjobs.com Featured Jobs, June 3, can be viewed online at: http://www.genesciences.com/DNAjobsNews/3June06.htm...
The amount of blotting or pre-printing needed for any microarray printing mechanism that dips into a source plate to load a sample is dependent on the volume...
Todd Martinsky
todd@...
Jun 4, 2006 2:32 pm
18390
... You can try this for the graphical representation: http://apollo.cs.uvic.ca/euler/VennCircles/ but for the actual calculations leading to classes etc, I'd...
Jose de_las_Heras
j.delasheras@...
Jun 4, 2006 2:32 pm
18391
Hi, I am going to validate my disease outcome data, however, I do not have an independent dataset, therefore, I am planning to perform a complete cross...
We have been using Corning "Pre-Cleaned Micro Slides" for years. It works great. The size is 75 x 50 mm Plain. Cat. No. 2947. We clean it with Ethanol and...
Xiuling Wang
GENE-ARRAYS@...
Jun 4, 2006 2:37 pm
18393
Dear Cliff, protein printing is sometimes associated with tip clogging, but is generally easily removed if you react quickly. In the manual of our...
Frank-Ulrich Gast
GENE-ARRAYS@...
Jun 4, 2006 2:39 pm
18394
... do try TMEV. It works well, and it's fast. Jose -- Dr. Jose I. de las Heras Email: J.delasHeras@... The Wellcome Trust Centre for...
Jose de_las_Heras
j.delasheras@...
Jun 4, 2006 2:39 pm
18395
Re - Dear Cliff, protein printing is sometimes associated with tip clogging, but is generally easily removed if you react quickly. In the manual of our...
Nigel J Saunders
nigel.saunders@...
Jun 5, 2006 8:28 am
18396
Hello all, Is anyone aware of a vendor that cells cDNA that has already been labeled with Cy3 or Cy5, or even amino-allyl-dUTP? Thanks, Kelly Kelly A. Shepard,...
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