Create individual labels for sample vials or microarray slides from NanoDrop data I am sure most of your labs have NanoDrop ND-1000 spectrophotometer for...
... Hi Nirmal; I was wondering; 1. in order to use 2 way ANOVA do I need to format data that is normalized (affy vhips) and saved in an excel format? 2. I...
Hi Kate: Here is feedback on some instruments I have direct experience with: Axon: In general is appears that the Axon systems have the most market share at ...
Todd Martinsky
todd@...
Jul 3, 2006 7:16 am
18514
Hello All, I just started working on my masters thesis work and got some microarray data to analyze After few tips, started working with BRB array tools and...
Krzysztof Zaphiropoulos
krzyz21@...
Jul 3, 2006 7:17 am
18515
Dear Colleague, You are cordially invited to attend the 5th International Computational Systems Bioinformatics conference, CSB2006, at Stanford University,...
lss-announce@...
Jul 3, 2006 7:17 am
18516
Krzysztof, You can convert log2 ratios to linear fold change by raising 2 to the power of the log2 ratio value: i.e. log2(treatment) - log2(control) = 3 ->...
Paul G Cantalupo
lupey+@...
Jul 3, 2006 7:19 am
18517
Hi, Isn't the subtraction in log2 scale equal to a division in regular values? Just de-log the resulting log2 value and you should have the fold change... ! Am...
Neuschaefer-Rube, Olaf
GENE-ARRAYS@...
Jul 3, 2006 7:19 am
18518
Dear Krysztof, In the case of positive values, I usually define Fold Change as Paul describes. however, when the log2(treatment) is lower than log2(control) ...
Juan C Oliveros Colla...
GENE-ARRAYS@...
Jul 4, 2006 5:19 am
18519
Hi Krzysztof, I'm sorry that the output of the class comparison is a bit confusing as regards "fold difference". With two classes the output gives the ...
Simon, Richard (NIH/N...
GENE-ARRAYS@...
Jul 4, 2006 5:20 am
18520
Dear Group, I am currently working with a research group interested in direct labeling of total RNA samples for hybridization with custom spotted microarrays....
DAVID WEAVER
GENE-ARRAYS@...
Jul 4, 2006 5:21 am
18521
Dear Dave, We currently use the Kreatech ULS system (http://www.kreatech.com) for all our labelings (RNA, DNA and protein) and are very happy with it. It does...
Dear David, It might be an idea to look at the labeling kits sold by our company Kreatech (www.kreatech.com). We use a direct labeilng technology for labeling...
a.mcgeever
a.mcgeever@...
Jul 4, 2006 11:03 am
18523
Hello Everyone, A quick note to update all of you as to the status of the Gene-Arrays List pending move. As you may know by now, the Gene-Arrays List is being...
Chandi Griffin
GENE-ARRAYS@...
Jul 5, 2006 5:27 am
18524
Dear Sir / Madam, we plan to print arrays from solutions of 1% polymer in organic solvent. Would a ring-and-pin spotter be a suitable instrument for this? Are...
Preininger Claudia
GENE-ARRAYS@...
Jul 5, 2006 5:27 am
18525
Dear all, I'm planning to do some oligonucleotide microarrays. The capture is amino- linked 20-mer oligos and it will be immobilized to SuperAldehyde slides...
We've been using Perkin Elmer's Micromax Direct Labeling kit for our direct labeling reactions and it has been working fine. Since I have never tried other...
Ying, Jun
jun.ying@...
Jul 6, 2006 6:16 pm
18527
Hi Claudia, It will depend of course on what the organic solvent you are using is, and how volatile it is. I doubt if the ring and pin system would be the best...
Steve Hawkins
GENE-ARRAYS@...
Jul 6, 2006 6:18 pm
18528
Hey all, Apologies to those who are already aware of this. Recently, Agilent has changed their bioanalyzer reagent kits to include the RNA ladder....
Hi Mike We have also been using Invitrogen Indirect labeling kits - both the "old" and the PLUS version - for several years with success. The major difference...
Hi Joan, From personal experience I would store the oligos in the spotting buffer which is normally 150 - 300mM sodium phosphate (pH8.5) ideally. I have in the...
stevefhawkins
stevefhawkins@...
Jul 6, 2006 6:33 pm
18531
Dave, If you are doing direct labeling, you most likely don't want to perform it on total RNA (unless you are monitoring rRNA and tRNA levels). For direct ...
GENE-ARRAYS@...
Jul 6, 2006 6:35 pm
18532
Micromax ASAP RNA labeling kit might be the product you are looking for. http://las.perkinelmer.com/Catalog/ProductInfoPage.htm?ProductID=MPS544001KT Kind...
Todd Martinsky
todd@...
Jul 7, 2006 11:43 am
18533
Dear Steve, Thank you very much for your advice. I still have several questions for you. Sorry for bothering you. How much should be one spot? I tried 2 ul of...
hai, We are about to start Microarray lab in India, i have doubt with respect to RNA isolation suppose if we isolate RNA from Cancer patients that includes the...
Hi Seth, and group, We have also just upgraded our software to accomodate the new kits. However I had not finished with my old "series I" chips and have been ...
Hello Kate, In addition to the instruments listed by Todd below, I would like you to know about the Tecan LS series of laser scanners. The Tecan LS is a highly...
Camilo Canel, T-US
GENE-ARRAYS@...
Jul 11, 2006 8:53 am
18537
Hi Seth, and group, We have also just upgraded our software to accomodate the new kits. However I had not finished with my old "series I" chips and have been ...
Hello group members, I was wondering if anyone has been using the Affymetrix MIDAS tool for exon analysis, and if they know the correct file format for the Cel...
David Carter
dcarter@...
Jul 11, 2006 8:57 am
18539
Hello Ashok, What type of samples do you have? If you tissue specimen from biopsies then you have to isolate RNA from the regions that have been diagnosed...
Hi David, there is a posting from Alan Williams on the Affy developers network EXACT forum. He explains how to strip out the directory information from the...
Having problems with message search?
Fill out this form to ensure your group is one of the first to be migrated to the new message search system.
