Dear all, In our lab we have the bioanalyzer from agilent and the nanodrop. When we check the quantity of RNA with these 2 machins, we never obtain the same...
We do a fair amount of work in that area. (http://pfgrc.tigr.org/) Could you please send me a resume and a brief background letter that describes your...
Hi Veronique: In our microarray core facility we have been using the Bioanalyzer and the NanoDrop together for three years. We usually get concentrations that...
I've noticed, here in the post MAQC <http://www.fda.gov/nctr/science/centers/toxicoinformatics/maqc/> days, when youd think the community would be a-buzz,...
Dear all, I would like to work on the miRNA array. Probe set and labelling kit are available from 3 societies: ambion, invitrogen and exiqon. I would like to...
Hello Veronique, I would also suggest you look at CombiMatrix miRNA arrays. http://www.combimatrix.com/products_microrna.htm They offer species specific miRNA...
Hi Veronique, I would suggest using Ambion kit. We had good results with Ambion kits for all array applications and they generally come with an internal...
Good Day to you! Thousands of couples broke down because of them, making both parties unhappy. The great thing about Extra-Time is that it works, making you...
Koenig
alfalfacharley@...
Oct 6, 2006 5:17 pm
18707
The Seventh Annual BCATS symposium and Industry/Academic Mixer will be held on October 21, 2006 at Stanford University. All members of the community are...
nikesh_k
nikesh_k@...
Oct 8, 2006 3:09 pm
18708
I work since 3 years in a company specialized in Biochip and i am now looking for a job in biotechnologies in Paris. If you have any contacts or if you are...
Hello, we are working on a project regarding an innovative microarray slide. The company has developped a microarray slide with a structured 3D surface...
Dear All, I have some problems for my microarray experiment (customize array with Cy3 and cy5 as dyes). Basically, most of spots are yellow and rarely red...
RNA later is consider best for storage of the tissue, but i would like to know the storage time, if you keep tissue sample for long time, RNA may be degraded ...
I don¹t think the problem is RNAlater. We extract RNA from both fresh, snap-frozen samples and samples stored in RNAlater and are able to label and hybridize...
I was told by the Agilent people not to use the Bioanalyzer for quantitations. I'd stick with the Nanodrop. It seems to be the best at it. last resort, run a...
Dear Andrew, with several years of experience using PIQOR and Agilent Microarray we could not see an effect on RNA/cDNA labeling efficiency upon sample storage...
kerstin.otterbach
kerstino@...
Oct 23, 2006 8:52 am
18715
We recently started using the Spotbot and we quickly found that the SpocleGen program that comes with it is very inflexible with regards to array placement and...
Hi all, Our lab (microbiology) has recently purchased custom-made GeneChips from Affy and we are soon going to start our first experiments. I was reading a...
if only it was that simple Marcus. Data analysis IS what micro-array is all about. doing the experiment is cake. figureing out what the data is and means is...
Hi- It seems that the price of gasoline has gone down here in Maine but Invitrogen and other companies are still charging the surcharge. Are prices down across...
Dear Marcus, your boss must be VERY naive.. regards halit Find out! <dalmuti@...> wrote: Hi all, Our lab (microbiology) has recently purchased custom-made...
We're trying to spot gamma globulins on Accelr8 slides using a Telechem SpotBot and SMP4 pins. We've varied printing buffer, Tween, BSA, humidity, etc and we...
I can probably help Accelr8. The problem is getting the correct amount of interface silane on the slide while keeping the slide and the silane totaly free of...
I just heard from a friend of mine who's working at the scripps sister site in florida and there core facility is running chips and doing basic data analysis...
rockclmber104
rockclmber104@...
Oct 24, 2006 7:13 pm
18723
Hi What printing parameters you are using in your printing experiments. How many spots/dip you are making. Are the donuts showing up at tail end of spoting...
... and the ... that are ... OD from ... contaminated ... Sometimes a ... sample ... sample in a ... from ... choose ... quantity-wise...they ... This is...
i am definitely agreed with this point. also the experiment is difficult,if you have not been to the trained class of AFFY.it just like the normal blotting...
Das ist doch Dein Spezi in Canada oder? Vielleicht könnte er sich für ein NanoPhotometer erwärmen. Gruß Thomas ... From: neil_winegarden To:...
Dr. Thomas Sahiri
sahiri@...
Oct 26, 2006 6:22 am
18727
Judd, Donuts on NHS activated polycarboxylate surfaces can appear if the surface is of moderate protein reactivity/capacity and at the same time affected by...
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