We're trying to print SSA, SSB, SCL70, Sm, and RNP on NHS activated hydrogel and are having a devil of a time. The methods successful with model systems (IgG,...
I actually have two questions. 1) Is it possible to print protein microarrays using in-house robots? We currently have the Microgrid 610 by Genomic Solutions...
hugo_villanueva@...
Nov 1, 2006 3:47 pm
18733
... Hi Hugo, 1) Theoretically yes (look at work for example by Gavin MacBeath who showed this was possible some time ago). The problem is not all proteins...
Judd, I am not a DNA Array specialist nor a biochemist, so I am lost in your terminology. Just in case, I am a specialist in surface tension control and the...
Hi Hugo, The brightness of spots depends on a number of things. Most importantly, how many pmols of incorporated dye are you using? I get good signal with 50...
Hi Hugo, You can look at my website http://www.uq.edu.au/~uqalicha/ScienceImages.htm#arrays to see the Alexa images or if you would want I can send you the...
Dear Judd and Hugu To give you both some answer in the same email: Judd, the comet tails might be to do with the amount of protein printed. This is often the...
Does anyone have experiences with Telechem 946 spotting pins and how they compare to other pins on the market? Are there cheaper pins which are just as good in...
Judd, SCHOTT Microarray Solutions provides a very nice protocol for printing on NHS ester activated hydrogels. Click or copy/paste the link below for the...
Dear all: We intend to create multiple arrays on one slide for diagnostic application using antigens extracted from cell lysates. It seems that non-contact...
hywang2001
hywang2001@...
Nov 3, 2006 5:22 pm
18741
The comet tails are a real problem and it definitely only shows up when the slides are washed and blocked. We've printed with .15M PO4 pH 8.5 with various ...
IF anyone has advice, that would be so nice. We are using the invitrogen indirect labeling kit with the telechem microarray probe purification kit. We are...
Judd, The comet tails are caused by electrostatic preconcentration and subsequent covalent coupling of excess protein on negatively charged sites around the...
It semms either a poor cDNA synthesis or low recovery at purification. I would start by looking at RNA quality. Run a BioAnalyzer trace see how good or bad is...
Dear group members, in a current project we are working on streptavidin applications. Does somebody here supply streptavidin coated slides? It would be very...
... Hello there, Out of curiosity, why are you using the Telechem clean-up kit rather than the one provided with the Invitrogen kit? I have nothing against ...
... Hi there, The 946 pins seem to be very good - as good or better than the original SMP pins. The loading collar makes them more convinient to work with....
We tried the 1M ethanolamine and still got the comet tails. We took the slides right from the dessicator and dropped them in a slide holder filled with it and ...
Dear Manfred, We offer streptavidin modified hydrogel slides. Compared to streptavidin adsorbed to unmodified 2D surfaces they show a higher streptavidin...
We use Parallel-Syn pins, and I agree with Neil. The only thing I'd like to add is that the silicon pins don't need to be removed nearly as much as the...
Judd, It is important that the quenching buffer on the slides is immediately and continously mixed during the quenching step as otherwise - as you noted - the...
Hi HY, I use to be the applications leader for the PerkinElmer Piezorray in Europe. I have spotted down several types of lysate, however in all cases I had to...
stevefhawkins
stevefhawkins@...
Nov 7, 2006 4:01 pm
18754
-Hi amyjanesmum, It's true that the Silicon Pins are delicate outside of their collimator/printhead support, and most microarray users have probably never...
Dear Andrew, I have seen bad RNA come from the tissue stored in RNAlater only once and the reason was pretty simple, we have not removed completely the...
I am getting a bit flummoxed here. I have to deal with this reviewer who reckons 18S is not an appropriate endogenous control because it is too stable. He also...
Does anyone have any experience with the biorad Experion as opoosed to the Bioanalyzer by Agilent? We are setting up a new lab and trying to decide between...
We do use the Bio-Rad Experion system and we are satisfied with the system. The sale service from Agilent was so bad that no one responded to our inquiry for...
Hi Ivana, We've been working with Experion system for approximately a year, and we're very happy with the performance. I have quite a lot of previous...
Hi Ivana: We have switched the software on our Bioanalyzer and are now using the Series II kits. Everything is smoother than ever. The change went very ...
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