qPCR 2007 - New deadline 12th February ... Dear colleagues, dear researchers, dear company representatives, On behalf of the Organisation Committee and the...
Dear All, Has anyone ever met a problem in the elution with Qiaquick PCR purification kit ?. Traditionally, for a direct labelling without amplification, we ...
Dear group Has anybody experienced any intermittentment high backgrounds using Affymetrix arrays, if so could you offer a reason as to why this happens (we...
Hi, I had some problems with Qiagen kit before and have a protocol that has worked for us. If you are interested I can send it to you, just drop me an e-mail. ...
Hi everyone, I'm currently involved in a microarray project isolating RNA from ICC and smooth muscle cells. Due to the limited quantity of cells obtained...
Dear microarray@yahoogroups.com members, We would like to announce two BS, MS or PhD level technician job offers that are currently open at our institute, the...
Hi Holger, We have been trying out NuGEN WT Ovation kit which actually amplifies your cDNA. We have got the yields a bit below what the company claims you...
There sis a kit from Epicentre now which can amplify Even RNA from 5-6 cells. Check out there website. Cheers praveen _____ From: microarray@yahoogroups.com...
Most companies supply info on RNA amplification kit linearity. From one point of view, RNA quantification is a relative science and as long as the trends are...
Hi Holger, Miltenyi Biotec is offering SuperAmp Service on Agilent Whole Genome Microarrays from 1-100000 cells. Please have a look on their homepage: ...
Jan Schäferkordt
JanSch@...
Feb 8, 2007 8:23 am
18879
I would suggest the Pico Ovation kit from NuGen. We have had some success with that product. You may be on the low end of the spectrum with only 50pg. There is...
This question is very important for me. It is quite expensive to test different kits, so it would be valuable for me if you all could help and share your...
qPCR 2007 - FINAL CALL Dear colleagues, dear researchers, dear company representatives, On behalf of the Organisation Committee and the Scientific Board it...
Just in case anyone uses these slides from Schott, there is a known batch problem with 4-1116. If you take the slides out, you will notice a film on the...
Hi Everybody, I am looking into getting a robot for RNA purification, also setting up RT-PCR and possibly array probe preparation in the future. I was browsing...
Hello Dr. Lichanska, Those three are reliable for large scale applications, and another is Hamilton; For moderate sized applications, you might also want to...
Dear all, We have a routine spotting method for short oligos which worked fine for the last 5 years or so: 50 uM oligo in 50% DMSO Cel Associates aldehyde...
I am trying to export partial of affybatch. For example, I have 12 affymetrix cel files. Then, I use ReadAffy() to import all of them as an affybatch. After I...
Dear Lev: Has the same plate set been used a number of times or is it a new set? DMSO can take on water over time, so it can look like lots of sample is left,...
Lev, as you know my company specialises in surface tension modification equipment. It has been our experience that if we correctly modify the surface for some...
abatch<-ReadAffy() # Reads all CEL files in a directory abatch.part<-abatch[,1:3] # The second index selects samples (here -- the first three) you need. The...
Hi Yan, This question would best be posed to the listserve dedicated to Bioconductor, as you seem to be using Bioconductor packages to do your initial...
hi friends, m trying to fix dna on glass slide. i followed as, 1.washing slides in H2So4:H2o2(2:1) for an hour. 2.washed in deionized distilled water. 3.dried...
Here is the problem. When you dip in APTES there is stillmoisture on the slide. The APTES reacts with the hydrogen in hydroxyl ions or the hydrogen in...
Hi, I'm working with 70-mer oligo arrays. They were spotted at 30uM in 3xSSC, and I'm noticing some degradation in spot morphology across the print run....
I need input! You see, we have been trying to get results for a few months now. First, we tried indirect aminoallyl labeling of our cDNA. We seemed to get...
Hi, The first question I would have is what is your starting material (tissue, cells, animal plant)? The second question is how are you preparing the RNA, I...
Hi Claire Here are my comments.... 1) By looking at the slides and data extraction, I am not sure how reliable the data will be. You get biased sometime while...
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