Hi, I am new here. I would like information on purification of probes after PCR before spotting. The general protocols I have seen seem to get away with...
I'm brand new to this site and I'm inquiring about self-made slides. Does anyone who use self-made slides using oligonucleotides and how complicated this...
bcmcneil18
bcmcneil18@...
Jan 17, 2008 8:58 am
19122
Hello all, I'm brand new here, but I'm in dire need of information regarding self-made slides. Has anyone ever used this for oligonucleotides and how...
Hello to all I'm a new array user and I would ask: how long can I use customized oligo arrays kept at room temperature?Should I have some problems in the image...
Clean and dry is the best storage. How good the microarrays work depends a lot on the surface chemistry used. If they were printed on poly-l-lysine slides...
Hi B, Don't be insulted by Todd's message. I doubt he's trying to belittle anyone's glass making abilities. I think he believes what he says, and my guess is...
Chris Seidel
seidel@...
Jan 21, 2008 12:20 pm
19126
Dear bcmcneil18 The reagents and chemicals are too nasty to bother with making your own slides. Just the process of getting good glass, and cleaning it to...
Hi Jamila, I would be surprised if they work but I would give a shot with one or two slides and see the damage that was done by storing at RT. However, to...
Hi, I'd like to have your feedback about telechem pins ( mainly 946MP3). Did you use them for a long time, how many spots did you achieve with the same batch?...
Hi Jamila, We spot 5' amino modified short oligos onto aldehyde slides (Cel Associates) and store them at room temperature in sealed plastic bags with...
Hi Jamila. One issue you don't want to overlook is that atmospheric 'debris'...everything from dust, pollen, stray chemicals and perhaps even ozone will affect...
Field Application Scientist The Field Application Scientist (FAS) is responsible for developing focused strategies and tactics for helping their respective...
jtrynak
jtrynak@...
Jan 23, 2008 8:22 am
19134
An extension on the answers by Dr. Levente, Dr. Lichanska, Dr.Seidel, and Todd Martinsky to Jamilla's question. This from a non DNA expert. My company has...
Chris makes some very good points. Any manufacturing process has a yield associated with it. There will be waste, so be prepared for a fair bit of QC work...
thanks again Ia appreciate the help ... own ... prepare ... source, the ... may be ... this E-mail ... disclosure ... not taken) ... more ... microarray...
To be more specific, the majority of my unique oligo sequences range from 18-25mers for oligont microarray. I'm not too sure if the direct parallel synthesis...
Thanks again Todd I apperciate the info. My oligos mainly range from 18-25mers, with the exception of a few 50mer. Anyhoo, do you recommend any journal or book...
Thanks again Chris appreciate the words of wisdom. My oligos mainly range from 18-25mers, with the exception of a few 50mer. I think depending on the length of...
Dear Erwan: Pin life depends greatly on the Z axis parameters of your microarrayer, pins can last much longer than what you're experiencing. We have...
... My Experience has been significant reduction in signal if primers are not removed - Usually standard purification spin columns are sufficient (Quiagen), or...
sarahdriver39
sarahdriver39@...
Jan 24, 2008 7:39 am
19142
18 bases is considered a "short" oligo. I would recommend using a C12 amino linker on the 5' end of the oligo and spot it out on SuperEpoxy2 surface ...
Dear Murali: Ethanol precipitation can be made to work, but if you're not really good at it will fail. Sarah is right, use a column based kit. There are...
I am interested in people's opinions about automation of microarray processing. As the arrays expand into the diagnostic laboratories they will require a...
qPCR NEWS - January 2008 - Special Topic: High Resolution Melt Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest...
Dear microarray users, I would like to get "the feeling" of experts dealing with SNP microarrays regarding sample size estimation in genome wide association...
phguardiol@...
Jan 30, 2008 3:59 pm
19147
Dear all, It appears to be generally assumed, that a 5' NH2 group on short oligos is bound preferably onto aldehyde and epoxy surfaces (vs. primary and ...
Dear Agnes: I wrote a blog entry on this topic. You may find it useful. http://arrayit.blogspot.com/2007/10/microarray-hybridization-manual-versus.h tml Kind...
Hi Brian, Many thanks for your help, and yes, it makes perfect sense. At least I believe I got it, which is quite something - the last time I felt I understood...
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