BOSC 2008 Call for Abstracts Reminder The 9th annual Bioinformatics Open Source Conference (BOSC 2008) will take place in Toronto, Ontario, Canada, as one of...
darin.london@...
May 2, 2008 8:06 am
19310
Todd, We just adapted the protocol for Agilent arrays. For home made protocols you may need the Fx because you run quickly out of deck-space on the Biomek3000....
Paul Van Hummelen
GENE-ARRAYS@...
May 2, 2008 8:08 am
19311
We use Agilent arrays a lot, but their Feature Extraction software seems quite overpriced. Are there anyone who knows of good very cheap/ free alternatives...
Kristian Almstrup
GENE-ARRAYS@...
May 2, 2008 8:08 am
19312
Dear Agnieszka For an affordable personalized automation system, WellAware pipetting now has microarray modules as well as PCR, sequencing and genotyping...
Dear Alexansra, I have not worked on Quantitative Antibody Arrays, but protein microarrys on several substrates including nitrocellulose slide, HydroGel coated...
GB wang
fgbw@...
May 2, 2008 8:08 am
19314
Hi Kristian, If you are using Agilent arrays there are many advantages that the Agilent Feature Extraction software offers that, given our knowledge of our own...
jeffrey_mcmillan@...
May 2, 2008 8:10 am
19315
Hello Alexansra, A new microarray slide printer prints any number of pads on any slide, including nitrocellulose and HydroGel. So you can use a single slide...
Dear Walter: Yes, you're correct you can change the stamp and make different patterns. We're also making custom patterns. It's a great tool and it is also fun...
I'm having a problem getting the minimum yield from the Affy WT kit for the Gene ST arrays. Our lab has successfully used the WT kit with 1ug of total RNA...
dreynold@...
May 6, 2008 6:51 am
19319
Dear all, I'm looking for expression data (preferably Affymetrix) from any Eukaryote where a region (covering as many genes as possible) has been either...
Per Stenberg
per.stenberg@...
May 6, 2008 6:51 am
19320
Dear Microarray Community Members: NextBio announced that a free version of the powerful life science search engine is now available to the entire scientific...
It have worked smoothly in our lab. Haiping ... [Non-text portions of this message have been removed]...
Haiping Hao
GENE-ARRAYS@...
May 7, 2008 5:26 am
19322
Dear Petr, We have a database of publicly available Arabidopsis Affymetrix data consisting of ~3000 arrays (http://affymetrix.arabidopsis.info/). In the...
Neil Graham
neil@...
May 7, 2008 5:26 am
19323
Look in GEO or other databases for data on renal cell carcinoma. Most of these tumors show whole chromosomes or whole chromosomal arms with...
Monzon, Federico A., ...
GENE-ARRAYS@...
May 7, 2008 5:26 am
19324
Dear Dave, I saw your mail about your problem getting enough amplification when using the 100ng protocol. I have now run some projects. They say in the...
Pia Karlström
Pia.Karlstrom@...
May 7, 2008 5:27 am
19325
Jing Sorry for delay in reply. In any microarray experiment, usual assumption is that there would be similar number of up-regulated and down-regulated genes,...
Mayank Kumar
mayank.k@...
May 7, 2008 5:27 am
19326
Hi Dave, I have worked extensively with the ST arrays from Affy and they were fine. The new kit is a big improvement in comparison to the old one from Affy....
Hi all, I¹d have to agree with Jeff on this one. For the Agilent arrays the Agilent software really is the best overall option hands down. It automates ...
Hi microarrayers I am new in the microarray field, I will use soybean long Oligonucleotide Array, in the literature there are two targets (cDNA and cRNA). What...
Hello there: We have successfully implemented the Nugen labeling protocols into our core, and have used them for a number of successful projects. However we ...
Dan McElligott
GENE-ARRAYS@...
May 9, 2008 7:39 am
19330
Hello How much does the Agilent software cost for academic labs? We are using PCR based slides with bacterial genomes. Will it work well with those? Susan ...
Boyle-Vavra, Susan
GENE-ARRAYS@...
May 9, 2008 7:40 am
19331
Software programs like Imagene from Biodiscovery process images in batch mode. They've been providing this type of software longer than any other company that...
Dear Dan, Are you also using the WT-ovation Pico RNA amplification kit? We do not have good reproducibility starting from 500pg total RNA. Have you done ...
Paul Van Hummelen
GENE-ARRAYS@...
May 12, 2008 5:39 am
19333
Hi Susan, If you have an Agilent scanner (version A, B, or the new high-resolution scanning version) or Axon 4000A/B scanner then the software will work. We...
jeffrey_mcmillan@...
May 12, 2008 5:39 am
19334
Depends which kits you are using. We have had good success with the standard Ovation but the Pico and FFPE kits are a nightmare. It took weeks and may lost...
Nicholas Webster
NWebster@...
May 12, 2008 5:39 am
19335
We were having some trouble with the NuGEN data that turned out to be the result of contaminating DNA. The labeling yields were good but the 3'/5' ratios were...
Steven Robert Head
shead@...
May 12, 2008 5:39 am
19336
Hi, I have seen much higher 5'/3'ratios for B-actin than I have seen with standard Affy amp/labeling (~ 12-15-fold) when beginning with high quality RNA. ...
Richards, Brenda
GENE-ARRAYS@...
May 12, 2008 5:39 am
19337
Respected Mayank sir As I am in hyderabad for project on microarray analysis in huntington's disease I need to meet you for many of the questions? Can you meet...
Hi Susan, While the Agilent Feature Extraction software will work with other arrays (such as your PCR based slides) it is not necessarily the best solution. ...
Hello Dan We use the NuGEN WT Ovation pico amplification kit in our core facility for some of our microarray work (hybridising mainly to human and mouse ...
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