Hello Everyone, There have been some changes made here at the host site of the GENE-ARRAYS List of which one is of particular import. The address for the...
Chandi Griffin
array_seq@...
Sep 10, 2008 7:15 am
19472
We run a microarray facility in the Netherlands where we print with 3 MGII robots. We print less slides now so we have one MGII too many. If anyone of you is...
Fellow microarray researchers, I am happy to announce that we have launched a web portal (www.microarraying.com <http://www.microarraying.com> ) dedicated to ...
A postdoctoral fellow position in computational biology will be shortly available at University of Washington, Seattle, WA. The candidate will join a...
Hi I am starting to learn running Gene Expression using NimbleGen arrays. I've had some 'issues' during the learning curve with (at the moment) the...
Caprice Rosato
rosatoc@...
Sep 25, 2008 8:29 am
19477
*Post-doctoral position : Sequence capture for targeted sequencing of genomic regions of interest: development in test studies in animal genomics* INRA Centre...
Dear Caprice: We have feedback from users that our "FPP" kit works with NimbleGen microarrays. http://arrayit.com/Products/Purification/FPP/fpp.html Kind...
For what it's worth I wouldn't feel restricted to their recommended protocol for RNA extraction. Use Tri Reagent or similar and clean up the aqueous phase...
Dear Caprice, We do a lot of NblG cDNA synthesis reactions and do not use the phase lock tubes for the cDNA purification step. We stopped after one week of...
Sandra Splinter Bondu...
sandrasb@...
Sep 29, 2008 6:50 pm
19482
Hi there, I have been using "SuperScript III cDNA labeling kit" from Invitrogen for two years. But I have been feeling unhappy at the long wait for the...
Hi guys, last days I was teaching and some student asked me why we can't use 2-color slides just with one color? And I guess my answer wasen't so convincent. ...
Hi Edgardo, Actually there really is not any reason you cannot use 2-colour slides with just one colour. In deed, Agilent refers to their platform as dual...
Hi Edgardo, I'm going to assume you mean using 2 color cDNA or long (50-75 mer) oligo arrays that are printed. These arrays could be used with just one dye,...
Hi Edgardo, you can use 1-color hybridizations (Affymetrix does it all the time...) but: -if you use home-made printed arrays the amounts of DNA in each spot...
Hi Edgardo, What Neil says is true - ratio data is on the whole much more stable than intensity data. There are however software tools which will allow you...
Dear Jean Ho. How about Amersham cyscribe first-strand cDNA labeling kit? http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocu ment ...
Hi Edgardo, The reason why 2-color slides can't be effectively used for single color assays is because the array spots have unique properties which make them ...
Chris Seidel
seidel@...
Oct 3, 2008 6:48 am
19492
Hi Jean, Ambion labeling kits, distributed now by ABI are great and they tend to be pretty good with delivery but I would always check if they have them in...
Dear all, I am looking for mycobacterium tuberculosis microarrays. Does anyone have an experiment with such microarrays? Thanks for your help. veronique -- ...
Hi Veronique, We, Genotypic Technology (AGILENT Certified Microarray Service Provider, R &D partner) have two validated catalog M. tuberculosis array...
Dear Veronique, We, Genotypic Technology (AGILENT Certified Microarray Service Provider, R &D partner) have two validated catalog M. tuberculosis array designs...
Hi, I'm just wondering if anyone has heard of a bovine micro RNA array - or knows if there is likely to be any cross reactivity with the human arrays ...
Hi Veronique, We offer mycobacterium tuberculosis through our National Institute of Allergy and Infectious Diseases. (NIAID) Our materials are free-of- charge...
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