Hi there, I have been using "SuperScript III cDNA labeling kit" from Invitrogen for two years. But I have been feeling unhappy at the long wait for the...
Hi guys, last days I was teaching and some student asked me why we can't use 2-color slides just with one color? And I guess my answer wasen't so convincent. ...
Hi Edgardo, Actually there really is not any reason you cannot use 2-colour slides with just one colour. In deed, Agilent refers to their platform as dual...
Hi Edgardo, I'm going to assume you mean using 2 color cDNA or long (50-75 mer) oligo arrays that are printed. These arrays could be used with just one dye,...
Hi Edgardo, you can use 1-color hybridizations (Affymetrix does it all the time...) but: -if you use home-made printed arrays the amounts of DNA in each spot...
Hi Edgardo, What Neil says is true - ratio data is on the whole much more stable than intensity data. There are however software tools which will allow you...
Dear Jean Ho. How about Amersham cyscribe first-strand cDNA labeling kit? http://www4.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocu ment ...
Hi Edgardo, The reason why 2-color slides can't be effectively used for single color assays is because the array spots have unique properties which make them ...
Chris Seidel
seidel@...
Oct 3, 2008 6:48 am
19492
Hi Jean, Ambion labeling kits, distributed now by ABI are great and they tend to be pretty good with delivery but I would always check if they have them in...
Dear all, I am looking for mycobacterium tuberculosis microarrays. Does anyone have an experiment with such microarrays? Thanks for your help. veronique -- ...
Hi Veronique, We, Genotypic Technology (AGILENT Certified Microarray Service Provider, R &D partner) have two validated catalog M. tuberculosis array...
Dear Veronique, We, Genotypic Technology (AGILENT Certified Microarray Service Provider, R &D partner) have two validated catalog M. tuberculosis array designs...
Hi, I'm just wondering if anyone has heard of a bovine micro RNA array - or knows if there is likely to be any cross reactivity with the human arrays ...
Hi Veronique, We offer mycobacterium tuberculosis through our National Institute of Allergy and Infectious Diseases. (NIAID) Our materials are free-of- charge...
Can you please post: Hi, We have previously lysed our tissue in Trizol and disrupted the cells using the Beadbeater/zirconia beads from Biospec Products....
Hello Veronique, Febit has arrays for this organism. You may try to contact them and check it. Greetings from Spain Guillermo ... Guillermo López-Campos ...
Hi Veronique, Depending what you are interested in. We do provide custom services for full genome sequencing arrays, if that is what you want to do. For...
... Hi Caprice, we just did a few expression experiments with Nimblegen. I just used my usual reagents & methods and it went quite well. For cDNA purification,...
j.delasheras@...
Oct 9, 2008 2:20 pm
19505
Hi Veronique, Take a look at the following sites regarding resources for pathogen research from NIAID. http://www3.niaid.nih.gov/research/resources/pfgrc/ ...
Braisted, John C.
braisted@...
Oct 9, 2008 2:20 pm
19506
... For completion... I extract RNA using Trizol, trying not to be too greedy and not getting too close to the interphase when taking the supernatant out. Then...
... Hi Caprice, we just did a few expression experiments with Nimblegen. I just used my usual reagents & methods and it went quite well. For cDNA purification,...
Hi Veronique, We also have a Mtb array. You can have more information on it at http://www.cag.icph.org/ You can also contact the director of the center Dr....
Hi Brenda et al., We have used a Biopulverizer for several years. Freeze tissue in LN2, smash to powder with the gadget, add the powder to Trizol and get on...
Have never done it. Could you try it out yourself? Pan did the last rna prep on hamster cheek. Do you know how it went? ________________________________ From:...
We plan to print microarrays in 96 and 384 glass bottom well plates. We need a good washer that won't leave comet tails around the spots. What do you guys...
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