I have a question about kit two-color Microarray-Based Gene Expression Analysis of Agilent. I would like to use this kit to amplify and label RNA for gene...
I Have a question about kit two-color Microarray-Based Gene Expression Analysis of Agilent. Since this kit uses a system of direct labelling I would like know...
ok ... From: Frank-Ulrich Gast - GeSiM <gast@...> Subject: [microarray-group] Re: Performance of Gesim Piezoelectric Arrayer To:...
Chandi Griffin
array_seq@...
Nov 3, 2008 9:14 am
19543
Kelly, Good question. Two ways are commonly seen: total signal intensity or house keeping gene controls. Total signal intensity used for normalization is...
daniel.johnson@...
Nov 3, 2008 9:14 am
19544
Hi all, For in-house spotting of 70-mer oligo arrays, what do you all prefer? We are looking to potentially upgrade from our current arrayer. Thanks, Heather ...
Dear Heather: I've written a blog entry that highlights some of the key considerations. http://arrayit.blogspot.com/2007/10/how-to-choose-microarrayer.html I...
Hello Heather, There are a few things that would help in offering an opinion you do not mention how many features per array you want to print, nor how many...
Agilent's two-color labelling kit: The labelled dyes are incorporated during the cRNA synthesis stage. The efficiency of labelling is comparable and equal...
Kelly, From our experience, miRNA is relatively more stable compared to mRNA. If your RNA samples have not gone through multiple thaw/freeze cycles, they...
daniel.johnson@...
Nov 4, 2008 9:50 am
19549
We are using Genespring to normalize Agilent's miRNA arrays and it works well. Raja www.genotypic.co.in From: kelljack13 <kellyannejackson@...> Sent:...
Hi everyone, I am trying to find out about Dart Array Technology. Most protocols mention usage of 1 microM of cy dyes. I have not been able to find a...
Madhuri.K
madhuri.k@...
Nov 4, 2008 1:32 pm
19551
Hi everyone, I am trying to find out about Dart Array Technology. Most protocols mention usage of 1 microM of cy dyes. I have not been able to find a...
Madhuri.K
madhuri.k@...
Nov 4, 2008 1:38 pm
19552
Hi Madhuri, For DArT array we label our targets using BioPrime® Array CGH Genomic Labeling System from Invitrogen (18095-011) and Cy-dCTP from Amersham ...
I've heard that the following company: http://www.spectrofuge.com/index.html Is providing service for the ESI/Virtek/BioRad microarrayer. So anyone who still...
Hello every one I am having problem in having good quality RNA from liver biopsy samples. I am using Qiagen RNeasy kit for this purpose according to...
Dear all, I am looking for arabidopsis and medicago microarrays. Does anyone have an experiment with such microarrays? Thanks for your help. veronique ... -- ...
Hi Neil: I've not used the ArrayJet, but I have learned from customers that if glycerol is not used in the print buffer the system does not spot well. Has that...
You want to make sure that your extraction method captures the miRNAs. Some of the column methods do not retain small RNAs. You can certainly visualize them in...
Dear Heather, I would like to encourage you to look into our dispensing technology. The reason is simple: if it comes to non-contact instruments, our spotter...
The key to RNA isolation from liver biopsies is to use a Polytron homogenizer for rapid and thorough tissue disruption. In addition, the isolation protocol...
Hi Neil: I've not used the ArrayJet, but I have learned from customers that if glycerol is not used in the print buffer the system does not spot well. Has that...
Thank you Tanja. I'' try this out. ... From: zuta_ludnica To: microarray@yahoogroups.com Sent: Tuesday, November 04, 2008 8:43 PM Subject: [microarray-group]...
Madhuri.K
madhuri.k@...
Nov 10, 2008 8:18 am
19562
Previously, I used to analyze gene expression with custom-printed and Affymetrix arrays and processed the data with simple custom analyses in Excel and MS...
All, Todd has his point to make as a vendor of a competitor technology, but I am concerned that he has perhaps given you all the wrong impression of our ...
There are a few ways to isolate good quality RNA from those samples. I used to work with biopsies but we did not need to use columns, for arrays obviously you...
Hi, I'm investigating gene expression olfactory tissue-derived neurospheres. I can easily harvest upwards of 1 million cells, yet my RNA yield seems to be low....
Hi Todd, Thank you for your input and let me address a few of your questions/comments. It is true that the ArrayJet unit requires some sort of reagent to...
Hi Duncan: The glycerol information I have about ArrayJet's spotting method is based on my technical support for our surface chemistry product line. If there...
Hi All, It has recently been brought to my attention that some inaccurate information about the Arrayjet spotters was published on this listserver. QUOTE: I've...
Hi, Any suggestion about scanner & software to view 3D view of microaaray spots after spotting.Is there any good way to perform anyalysis from spot view in...
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