Hello all, Please, I would like to know if there is someone that used 4000B Axon scanner and GenePix Pro 6.0 to scan Agilent two-color 4x44k microarray. I know...
Technical Assistant/Associate position at the Whitehead Institute/MIT, Cambridge MA Seeking highly motivated individual to play a key role in a cutting- edge...
tomvolkert
tomvolkert@...
Dec 3, 2008 8:57 pm
19586
Hello all, I have a question about the kit two-color Microarray-Based Gene Expression Analysis kit"Â of Agilent. I amplified and labelled total RNA from two...
Hi, Two reasons for that: 1/ The T7 polymerase has not the same yield when incorporing Cy3 and Cy5. For Cy 5 it's lower. 2/ Cy3-CTP inhibits T7 RNA polymerase...
Hello, Does somebody know the price for a license to use the Affymetrix technology in the context of pharmaceutical company? Thank you for your answers. Best...
Hello, The Emory Transplant Center Research Lab, located in Atlanta, GA, is seeking a senior research specialist in molecular biology. This position is in a...
Hi Pam, I am trying to access Genespring GX 10 remotely using my laptop from home, but it's not allowing me to access it. Does the program have any ...
Dear Erica, I did many scans of Agilent 4x44k slides and yes, Axon GenePix 4000B has enough resolution. But I never used Agilent scanner so I cannot give any ...
Marek Skoneczny
kicia@...
Dec 11, 2008 12:23 pm
19592
Erika, As the Agilent protocols call for adding specific amounts of cRNA, not amounts of dye, the balance between arrays on the same slide can potentially be...
markband@...
Dec 11, 2008 12:23 pm
19593
Hi Catherine, We extensively use Affymetrix license and are certified providers of Affymetrix platform. Please write directly to me (mayank.k@...). I...
Hello all, does anyone knows if a software exists that find out the homologue genes among different organisms submitting directly a list of gene codes? I have...
Hello all, We are helping a lab find a new home for their Illumina BeadStation 500G microarray scanning system. It includes the Illumina BeadArray reader, PC...
erincurry2
erin@...
Dec 16, 2008 8:47 am
19596
ABRF 2009 | Application and Optimization of Existing and Emergi ABRF 2009: Application and Optimization of Existing and Emerging Biotechnologies will be held...
Hi Erika If you have the list of genes from yeast, you can retrieve their sequences from genbank and perform a BLAST search against the human database using...
Willy Valdivia
willy_valdivia@...
Dec 16, 2008 8:47 am
19598
Hi Erika, Provided the SGD IDs are available in EntrezGene (which i don't know for sure), the homologene info within Entrez Gene can be retrieved for lists ...
Hooiveld, Guido
Guido.Hooiveld@...
Dec 16, 2008 8:48 am
19599
Hello, Have you tried Batch Entrez (http://www.ncbi.nlm.nih.gov/sites/batchentrez? ) If you have the accession numbers for those genes I guess you may query ...
HI I cannot switch of the finding (f6) of the whole block during batch analysis with Genepix6. Either find/align or nothing. Finding results (we are working...
Krainer Siegfried
Siegfried.Krainer@...
Dec 24, 2008 6:28 am
19602
I am working on microarray data mining in rice. Now, I wonder how to use Geneontology for my research work. The reality is that I have much data from...
xwzhang1112
xwzhang1112@...
Dec 27, 2008 1:30 pm
19603
Hello I would like to have your opinion about the way to analyse gene expression data "around transcription factors": there are biological situations in which...
Philippe Guardiola
allogreffe@...
Jan 12, 2009 8:27 am
19604
Dear all, does anybody know the density of probes in Agilent chips produced by the sureprint technology in molecules/cm^2? I know that photolitography in Affy...
hello I would like to have your opinion about the way to analyse gene expression data "around transcription factors": there are biological situations in which...
phguardiol@...
Jan 12, 2009 8:27 am
19606
I have both in my lab. The qArray2 works a lot better than microgrid II, although they are about the same expensive. ... and ... spotter,...
b.troy108
b.troy108@...
Jan 12, 2009 8:27 am
19607
All, My company (www.GenPathDiagnostics.com) has an immediate opportunity for someone to run our microarrray program. It is a clinical diagnostic laboratory,...
Does anyone have a suggestion about how to begin analyzing data generated using Methylation IP on NimbleGen promoter arrays? Are there any resources that the...
Caprice Rosato
rosatoc@...
Jan 13, 2009 1:03 pm
19609
Hi, I work in a small microarray laboratory in Poland, where we use SpotArray 24 (PerkinElmer) to produce DNA and RNA microarrays. I am going to buy a new...
Hello Philippe, It's a very interesting problem. Since last 6-7 years, researcher's have been working on determining the TF(s) that govern the expression of...
Hello Everyone. I am using oparray human V4 chip for my gene expression experiments. I am unable to get the good hybridization result although the labeling and...
Is there anybody who can advise me on current possibilities to run ChIP-on-ChIP (or MeDIP) on limited amounts of DNA/cells. What I have found as the lowest is...
Kristian Almstrup
kristian@...
Jan 20, 2009 10:50 am
19613
Hello all, In my reseacrh group we are using Agilent dual-color 4x44k whole human genome microarray for an experiment of gene expression. It is the first time...
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