Hello I would like to have your opinion about the way to analyse gene expression data "around transcription factors": there are biological situations in which...
Philippe Guardiola
allogreffe@...
Jan 12, 2009 8:27 am
19604
Dear all, does anybody know the density of probes in Agilent chips produced by the sureprint technology in molecules/cm^2? I know that photolitography in Affy...
hello I would like to have your opinion about the way to analyse gene expression data "around transcription factors": there are biological situations in which...
phguardiol@...
Jan 12, 2009 8:27 am
19606
I have both in my lab. The qArray2 works a lot better than microgrid II, although they are about the same expensive. ... and ... spotter,...
b.troy108
b.troy108@...
Jan 12, 2009 8:27 am
19607
All, My company (www.GenPathDiagnostics.com) has an immediate opportunity for someone to run our microarrray program. It is a clinical diagnostic laboratory,...
Does anyone have a suggestion about how to begin analyzing data generated using Methylation IP on NimbleGen promoter arrays? Are there any resources that the...
Caprice Rosato
rosatoc@...
Jan 13, 2009 1:03 pm
19609
Hi, I work in a small microarray laboratory in Poland, where we use SpotArray 24 (PerkinElmer) to produce DNA and RNA microarrays. I am going to buy a new...
Hello Philippe, It's a very interesting problem. Since last 6-7 years, researcher's have been working on determining the TF(s) that govern the expression of...
Hello Everyone. I am using oparray human V4 chip for my gene expression experiments. I am unable to get the good hybridization result although the labeling and...
Is there anybody who can advise me on current possibilities to run ChIP-on-ChIP (or MeDIP) on limited amounts of DNA/cells. What I have found as the lowest is...
Kristian Almstrup
kristian@...
Jan 20, 2009 10:50 am
19613
Hello all, In my reseacrh group we are using Agilent dual-color 4x44k whole human genome microarray for an experiment of gene expression. It is the first time...
Hi Caprice, We recently established an Epigenomics Facility that offers sample processing and analysis support for Methylation IP on NimbleGen promoter arrays....
George Grills
gsg34@...
Jan 23, 2009 2:35 pm
19616
Why not consider the NanoPrint? http://www.arrayit.com/Products/MicroarrayI/NanoPrint/nanoprint.html Kind regards, Todd...
Hi Evan, The main problem with the NanoVue is the glass surface that the sample is measured on. This can easily become stained by reagents such as Bradford...
jimblob74
james@...
Jan 23, 2009 3:33 pm
19618
Hi Evan, If you want to avoid recalibration and maintanance cost check out the NanoPhotometer from Implen. You will get the flexibility of a built-in computer...
Hi Evan Another option to consider is the Picodrop Spectro, this new option detects microliter samples directly in the P10 pipette tip so zero cross...
jonredfern@...
jr@...
Jan 26, 2009 7:25 am
19620
Evan as detailed in the other replies both nanovue and nanodrop use a similar technology i.e. trapping a drop between 2 fibers. nanodrop relies on forming a...
jonredfern@...
jr@...
Jan 26, 2009 7:26 am
19621
hello all: would anyone tell me how many spot numbers of present calls when you extracted total RNA from human PBMC and performed cDNA microarray. and would...
The NanoPhotometer is the best unit I have seen so far. The micro-fluidic cell used to take measurements is far superior to that of the systems that rely on...
Dear colleagues, We, the Gulari research group (Chemical Engineering Dept., University of Michigan), have developed a new in situ oligonucleotide synthesis ...
Hello Philippe, It's a very interesting problem. Since last 6-7 years, researcher's have been working on determining the TF(s) that govern the expression of...
Hi Erika I may be able to help, but can you tell me how you determine the percentage of spots discarded? Wook...
jwahn@...
spamkie@...
Jan 28, 2009 7:37 pm
19626
Erika, I am not sure from your message exactly how you are determining the low SNR for these arrays, but can certainly share some of our experience, and what...
stephanie_fulmer-smen...
Jan 28, 2009 7:37 pm
19627
Hi Caprice, here is a publication about dealing with NimbleGen promoter array MeDIP data: Genome Res. <javascript:AL_get(this,%20'jour',%20'Genome%20Res.');>...
Alexander Eckehart Ur...
alexander.e.urban@...
Jan 28, 2009 7:38 pm
19628
Hi! Happy new year for you! I did a timecourse experiment: varieties: Resistant and Susceptible treatments: Inoculated and Control (not inoculated) Time...
Hi! Happy new year for you! I did a timecourse experiment: varieties: Resistant and Susceptible treatments: Inoculated and Control (not inoculated) Time...
... you ... because ... appreciated. ... dear Daniel: thanks for your reply, I performed the two color cDNA microarray,the samples were total RNA comes from...
Hi, for each spot, I calculate the SNR as ratio between the bg subtracted foreground and the standard deviation of bg. Low SNR is above 3. I used Feature...
Try Picodrop - picodrop.com (I'm not sure) It uses a optical pipette tip. Measurement directly in pipette tip! No calibration of the optical way. No screws. No...
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