Hi, for each spot, I calculate the SNR as ratio between the bg subtracted foreground and the standard deviation of bg. Low SNR is above 3. I used Feature...
Try Picodrop - picodrop.com (I'm not sure) It uses a optical pipette tip. Measurement directly in pipette tip! No calibration of the optical way. No screws. No...
mikolaj.mikolaj
ofcourse@...
Feb 2, 2009 3:35 pm
19633
Alexander, Thank you so much. Caprice ... S. Caprice Rosato <rosatoc@...> Center for Genome Research and Biocomputing Oregon State...
Caprice Rosato
rosatoc@...
Feb 2, 2009 5:07 pm
19634
Hello all, I was wondering if alternative protocols exist for hybridization, washing and scanning of Agilent expression chips. I found some posts that discuss...
chris.voolstra
chris.voolstra@...
Feb 9, 2009 9:22 am
19635
Hi Evan, If you want to avoid recalibration and maintanance cost check out the NanoPhotometer from Implen. You will get the flexibility of a built- in computer...
andibauer78
andibauer78@...
Feb 9, 2009 9:23 am
19636
qPCR NEWS January 2009 - Lab-on-Chip special edition ... Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in...
Hi Luiza we are working with a Microgrid II aswell as a QArray2 and an Omnigrid Accent in our lab. Actually all systems are working fine, but the QArray2 is by...
zerweckj
j_zerweck@...
Feb 10, 2009 10:14 am
19638
Hi Luiza, If you are considering the Genetix, I will recommend as strongly as I can that you go with the 2 instead of the mini. I used to work for Genetix...
Hello all, I am scanning Agilent 4x44k Agilent two-color gene expression microarrays by using Axon scanner 4000B and Agilent scanner also. We use Quick Amp...
qPCR 2009 Event Agenda is online => http://online-agenda.qpcr2009.net/ The event is organized jointly by Chair of Physiology, Technische Universität München...
Hi, Could anybody share experience on printing protocol for oligos on epoxy coated glass slides? Is there any change in buffers and over all protocol when we...
If you use an epoxy coated glass slide without an amino linker you are prey to moisture problems. Moistute sets up an invisible barrier that the oligo rests...
Dear Mayank: Our micro spotting plus printing buffer is compatible with amine, aldehyde and epoxy silane surfaces, with and without linkers. In my experience...
Hi Mayank, It depends on the exact surface used before and the one you are switching to. We found that epoxy surfaces tend to be more hydrophobic than, say,...
Hello all, Can anyone help me with the following problem I'm facing? For my project, I need to integrate common information (genes) of 4 platforms for the...
rnayana6
rnayana6@...
Feb 12, 2009 12:46 pm
19646
Hi there, Do you know how much UV cross link I should do for poly-lysine slide or corning slides? It seems 60mJ for polylysine and 300mJ for croning slides. I...
Lev, I am wondering if you really use a solution containing only 50% DMSO and 0.01% SDS for spotting aminated oligos on epoxy-coated slides. I am not sure that...
Chizhikov, Vladimir
Vladimir.Chizhikov@...
Feb 12, 2009 3:47 pm
19648
Dear Vladimir: I'm sure your chemistry is correct, however, in practice we find very good binding and reaction kinetics under neutral pH conditions. The nice...
Hi Vladimir, Thanks for the corrections! Now that you say this, I remember my chemist colleagues mentioning the same... I should add, for the few epoxy slide...
Dear Researchers, Thank you for your affirmative replies. Appreciate your help for sharing your experiences. Vladimir, We use epoxy slide from Corning. We are...
Lev, You are absolutely right regarding the low effect of pH on immobilization aminated oligos on aldehyde-coated slides. pH is not as important as for epoxy...
Chizhikov, Vladimir
Vladimir.Chizhikov@...
Feb 13, 2009 3:11 pm
19652
Dear Lev: If I remember correctly, your application is using pretty short oligos. I think you are smart to try to stay with an aldehyde chemistry to get ...
Hi Jean I know a little about Corning arrays (UltraGaps and Epoxide chemistries). For UltraGaps the amount of energy depends on whether the features spotted...
Caprice Rosato
rosatoc@...
Feb 23, 2009 8:30 am
19654
Dear Jean: Some of our customers report the best signal to noise ration with a combination of baking and crosslinking. I believe this is because very dry ...
Our company recently acquired four Affymetrix GeneChip Fluidics Station 450 washers. We're now trying to find them new homes and are taking offers. Please...
Hi, We have a 10K array made for a specie X. But, we have used it to evaluate expression on specie Y. Before, we try to validate this array for specie Y. So,...
dear all, We recently acquired the Affymetrix station and tried to used the Genome-Wide Human SNP Array 6.0, but we systematically obtained poor quality of...
Marcelo, Could you please elaborate more on it. Let me share our experience which we do in our work. To validate X array to Y array in the form of linearity, ...
... Dear Mayank Kumar We have a 10K array for Bos taurus taurus and we would like to validate it to Bos taurus indicus. Based on your advice, we don't need to...
Hi Veronique, I observed similar pattern on some other Affy arrays, it seems to be a problem with manufacturing, contact Affymetrix and ask to get arrays from...
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