Hi frens, I have so many problems in life. I wud be a happier person if any one of you could please help me to solve at least the following: I obtained...
Hi, I am trying to isolate RNA from very small embyonic mouse tisse, that I cut out of the embryo for a microarray experiment. It is so tiny that it is barely...
nadjamakki
nmakki@...
Mar 3, 2009 4:38 pm
19664
Have you considered stabilizing the entire embryo or, at least, an easy to cut chunk in RNAlater before doing the final dissection? ...
Seth Crosby
scrosby@...
Mar 4, 2009 12:04 pm
19665
what embryonic stage are you working on and are you dissecting a single tissue out of the embryo? We worked with microdissection material and RNaqueous...
HI Krishan, I think you will not like my response to that: go back and repeat the experiment. I think that it would be the best thing to do. 4. While you can...
qPCR Event Newsletter - March 2009 ... Dear researcher, dear Gene Quantification page reader, We want to inform you about the upcoming qPCR events, workshops...
We've had a lot of good luck using the Arcturus PicoPure kit for RNA isolation small samples such as you are describing and always include the on column DNAse ...
Christopher Barker
cbarker@...
Mar 10, 2009 8:18 am
19669
Dear microarray users, We want to buy a new high resolution microarray scanner (2 microns). We are currently working with different slide providers (Agilent,...
Dear Stephane: I urge to take a good look at the Innoscan 700, adjustable down to 3 um resolution or the Innoscan 900 adjustable down to 1 um resolution. Both ...
PhD Studentship on Functional Genomics and Systems Biology of Mycobacteria-Macrophage Interactions in Cattle NB: this position is restricted to European Union...
We've had a lot of good luck using the Arcturus PicoPure kit for RNA isolation small samples such as you are describing and always include the on column DNAse ...
Hello. My company (SiMPore, Inc.) has developed a high-performance, ultrathin, nanoporous membrane that can potentially be used in a variety of applications. ...
Dear Listers, I am looking for data sets in which more than two tissue have been analyzed from the same patient like blood cells, muscle, and fat, or normal...
Hello Stephane, I cannot speak to the specific Axon model you speak of but I can tell you that our experience with Axon scanners has been quite positive - they...
Hi Neil, Thank you for your answers about the Agilent 2 microns scanner. In fact my main concern is about computer requirements and analysis time. Agilent is...
I'm currently planning new microarray experiments but would like to test my samples on test arrays to show a proof on concept to my collaborators. Does...
Hi again Stephane, Actually we did not upgrade our computer when we upgraded the scanner itıs currently only a 2.13 CoreDuo (32-bit) with only 2GB of RAM....
Dear Stephanie, We too are looking to purchase a 2um scanner to use with NimbleGen's HD2 arrays. We have done one HD2 array that we were able to scan on our...
Sandra Splinter-Bondu...
sandrasb@...
Mar 24, 2009 8:11 am
19682
Dear Sandra, I am not used to Nimblegen scanner for the moment. What I have done so far is scan one 244k Agilent arrays on three different scanners: GenePix...
I 100% advocate getting your own computer - we had enough expertise in our lab to build one, and thus we saved money but still got power where it counts. RAM...
Hi Neil, Thank you for your answer. I am quite impress that Feature Extractor is able to process a 1M Agilent slide with only 2Gb RAM. This means that it don't...
The Innoscan systems we're providing using windows XP 64 bit or Vista, we can set the image acquisition and quantification software to use as much RAM as we...
Hello, I have a question about Cy- labelled RNA conservation: I have Cy3-labelled RNA samples at -80 since three months (these samples have never been...
Hello, Try to quantify with the nanodrop in Microarray mode. Calculate the activitiy of enzyme. If you have good yield and you can hybridize some chips with...
Dear Stephane, Dear Sandra, My two cents regarding extraction of Nimblegen rectangular features using GenePix. I did not analyse Nimblegen arrays myself, but...
Marek Skoneczny
kicia@...
Mar 27, 2009 8:18 am
19689
qPCR NEWS March 2009 - focus on HRM & HRM dyes ... Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news in...
Hi Stefan, You are welcome of course BUT I realise I may have mislead you unintentionally. We have not actually processed a 1M feature chip. We have ...
Hi Everyone, Does anybody have experience with Affymetrix SNP 5.0 array? Half of our samples couldn't pass the QC. We don't know what's the reason. Your help...
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