Hi Kaiman, At which stage did they not pass QC? As the reasons can be quite different. Agnieszka ... Agnieszka Lichanska, PhD, Staff Scientist, TessArrae, LLC ...
I am about to do microarray preps on mosquito RNA samples that have been sent to me. I have a bioanalyzer trace and have been told that insect RNA looks quite...
Dear all, does anyone know what is the threshold for %CV of replicated probes and for local background level on Agilent microarrays? These parameters are from...
Dear all, We're looking for feature extraction software that can handle home spotted arrays scanned using our new 2 micron Agilent scanner. We fint Agilents...
I have been asked to process a number of mosquito RNA samples, and am hoping there might be someone in the microarray listserve community that would be willing...
Hi Kate, I’ve done quite a bit of mosquito RNA work and used a Bioanalyzer for QC. I might be able to help if you sent me the electropherograms, preferably...
Dear Martin: I've been in the microarray industry for 15 years and I like BioDiscovery and ImaGene for many reasons. I very much like the fact that they have...
Hi all, I have a question on using the Agilent 4X44K microarrays (G2519F, rat whole genome) with the MAUI Mixer A4 hybridization chambers - specifically on the...
Imagene from biodiscovery ? forget it ! try GENESIFTER ! they R the best (based on personal experience) http://www.genesifter.net regards h canatan ...
Todd,
Please remember to add "Commercial" to your replies if you are going to speak about software that your company sells (Mapix). This thread involves...
Dave, Varshal
Varshal.Dave@...
Apr 23, 2009 7:51 am
19704
Hi Martin, I have used ImaGene quite a lot a few years back when I worked at UQ in Brisbane and it was a very nice software easy to use, easy to automate most...
________________________________ From: Forum: microarray and related technologies on behalf of erika.melissari Sent: Tue 4/7/2009 6:25 PM To:...
odilo_mueller@...
Apr 24, 2009 11:32 am
19706
Hello All, I am new student working with affymetrix gene expression data comparing few mutations in human cell lines. I am looking for freewares for the data ...
Noel Mathur
noelmathur@...
Apr 24, 2009 6:59 pm
19707
Noel, We use LIMMA for all Affy data. Though LIMMA is a command driven package but menu driven interfaces are also available. See limmaGUI...
Hello, I am currently trying to establish a Microarray protocolwhere we want to hybridize labeled RNA to our cDNA microarrays.First experiments show to much...
markusvormann
markusvormann@...
Apr 29, 2009 2:45 pm
19709
qPCR NEWS April 2009 - focus on microRNA normalisation ... Dear researcher, dear Gene Quantification page reader, Our newsletter informs about the latest news...
Hi, I have a question regarding calculating the p value. For example, I have two biological replicate samples, in sample 1, for a gene A, I got a p value ...
Hi Markus, I am not sure what type of hybridization buffer you are using. You can try the temperatures up to 65oC with or without formamide. Aqueous solutions...
Dear Markus: Often high background is as simple as using the wrong blocking procedure, or having the hybridization dry during the reaction. Another factor is...
Hi all, I would like to draw your attention to a new method for removing dye-bias related artifacts in two-colour microarrays. The novelty of the method is...
Hi Markus, I am not sure what type of hybridization buffer you are using. You can try the temperatures up to 65oC with or without formamide. Aqueous solutions...
Hi Everyone, I'm a new member to this group, and in process of an experimental design to do an exploratory study for salt tolerance in Brassica juncea using ...
Hello All, I am curious to know if there is any rule of thumb when you should use RMA or GC-RMA normalization and what are the comparative advantages and ...
Noel Mathur
noelmathur@...
May 9, 2009 5:48 am
19717
Hello All, Now that I have affymetrix data and I have started analysis, I am facing following questions, so far googling hasn't helped much. 1. Is there any...
Noel Mathur
noelmathur@...
May 13, 2009 4:54 pm
19718
Hi Noel, It is normal that if you use MAS5, RMA and GC-RMA you end up having different results. However, you may still get some genes/transcripts in common if...
Hi all!! I am Lilian Oliveira and I am trying to analyze my micrarray data using that JNP genomics. I ran an experiment using the bovine 4x44 agilent...
... Hi Lilian, Did you have tried bioconductor's approach to microarray analysis? There are some QC methods and a lot of normalization methods there. To decide...
Hi, Does anybody know how to merge replicate spots in R Bioconductor? I use home-made spotted two-color arrays and every probe is printed in triplicate. I...
Hi friends, www.genetoday.com is a networking site for biomedical science professionals. Here one can meet other professionals in the same field, can upload...
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