Hi Markus, I am not sure what type of hybridization buffer you are using. You can try the temperatures up to 65oC with or without formamide. Aqueous solutions...
Dear Markus: Often high background is as simple as using the wrong blocking procedure, or having the hybridization dry during the reaction. Another factor is...
Hi all, I would like to draw your attention to a new method for removing dye-bias related artifacts in two-colour microarrays. The novelty of the method is...
Hi Markus, I am not sure what type of hybridization buffer you are using. You can try the temperatures up to 65oC with or without formamide. Aqueous solutions...
Hi Everyone, I'm a new member to this group, and in process of an experimental design to do an exploratory study for salt tolerance in Brassica juncea using ...
Hello All, I am curious to know if there is any rule of thumb when you should use RMA or GC-RMA normalization and what are the comparative advantages and ...
Noel Mathur
noelmathur@...
May 9, 2009 5:48 am
19717
Hello All, Now that I have affymetrix data and I have started analysis, I am facing following questions, so far googling hasn't helped much. 1. Is there any...
Noel Mathur
noelmathur@...
May 13, 2009 4:54 pm
19718
Hi Noel, It is normal that if you use MAS5, RMA and GC-RMA you end up having different results. However, you may still get some genes/transcripts in common if...
Hi all!! I am Lilian Oliveira and I am trying to analyze my micrarray data using that JNP genomics. I ran an experiment using the bovine 4x44 agilent...
... Hi Lilian, Did you have tried bioconductor's approach to microarray analysis? There are some QC methods and a lot of normalization methods there. To decide...
Hi, Does anybody know how to merge replicate spots in R Bioconductor? I use home-made spotted two-color arrays and every probe is printed in triplicate. I...
Hi friends, www.genetoday.com is a networking site for biomedical science professionals. Here one can meet other professionals in the same field, can upload...
We have done microarrays for wildtype-control treatment, wildtype-toxin treatment, mutant-control treatment and mutant-toxin treatment. The goal is to find...
Hi, I'm looking for a microarray scanner. We are planning to use at least 100 HEEBO slides per year with amplified RNA from human tissues and we are a medium...
Edgardo Salvatierra
ESalvatierra@...
May 25, 2009 8:25 am
19725
... Hi, In limma you could do something like this: (wildtype_treated)-(wildtype_control) -> genes differentially expressed in wildtype treatment dependent ...
I like the Innoscan 700A, makes great scans amd the Mapix quantification software is very easy to use. It is a networked device, is the fastest scanner...
Hello, We've recently found that VERY small changes in our PMT settings will have large changes on our end data when doing CGH using the Nimblegen platform. Is...
You should seek out a statistician with plenty of experience in ANOVA analyses, what you want to detect is whether or not there is an interaction. It is...
O. Colin Stine
ostin001@...
Jun 1, 2009 5:21 am
19730
Dear colleagues, Please note the announcement below for a workhsop on microbial diagnostic microarrays. The workshop will be a full 5-day hands-on course, held...
Prezado Marcelo Laia Meu nome é Renata Real, sou gerente de produtos da empresa RChisto Soluções Ltda, representamos oficialmente a Tecan no Brasil. ...
Renata Real - RChisto...
renatareal@...
Jun 5, 2009 5:06 am
19732
Everyone, I am once again going to ask that blatantly commercial posts, like the one below be marked as "COMMERCIAL". This was a very heated debate a few...
Hi I am new to microarray and have started using Mouse Affy GeneST arrays. I was wondering what software/packages do you use for such arrays. I need to...
Paul
anirban_paul@...
Jun 7, 2009 7:15 am
19734
Hi Paul, Please realize the Gene ST arrays do not contain mismatch probes anymore. MAS5 normalization (nor GCRMA) is therefore not possible. I know most about...
Hooiveld, Guido
Guido.Hooiveld@...
Jun 8, 2009 8:10 am
19735
Dear all, What is considered to be the best strategy to normalise bacterial whole genome arrays? The last time I thought and read about this it was either the...
Dear All on the Listserver, For making my food intolerance arrays I have a requirement for a red BioBank for the BioRobotics / Genomic Solutions / Digilab...
Centre for Genome Research, University of Liverpool As a result of substantial funding from MRC and other agencies the former Liverpool Microarray and Advanced...
Thank you Bart, I appreciate your comments very much. Like many of us on the list-serve I do love microarrays and all the great things they can do. Kind...
While I agree with you I kinda think Todd deserve a bit of leeway on this. He has over the years given a heap of really good non-commercial advice on arrays to...
You are correct of course. Next post I will remember to identify it correctly. Kind regards Todd Sent from my Verizon Wireless BlackBerry ... From: "Dave,...
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