Dear Jing, Genotypic technology, India also provides E.coli microarrays. They have a list of catalog designs and also offer custom designing services for...
Hello all! I have ran an microarray experiment analysis comparing monocytes from blood to macrophages from endometrium from pregnant cows. I used the bovine...
Hi, I m not sure it is useful to apply 2 normalization processes to your data, one of the 2 you used should be enough. Just check what the effect of each is on...
phguardiol@...
Jul 6, 2009 10:50 am
19763
Last point I forget is about keeping in your selected genes those that are with a fold change > 2, a nice p value, but a signal that remains in the "noise" or...
phguardiol@...
Jul 6, 2009 10:51 am
19764
Dear Adrian I'm the tech contact at Arrayit. Let me know what type of microarray you are planning and I can tell you which system we have will be best to meet...
Hi Lilian, The ontology analysis can be difficult and even with human genes you can get significant problems. DAVID used to take the GeneIDs as input as well,...
We have been having a real problem labeling yeast (C. albicans) RNA for the last few weeks. The RNA looks fine by bioanalyzer but the single strand labeling...
Hi I am going to use Nimblegene expression array (custom array) in my project and I am wondering if I could use easily GeneSpring Software to analyze...
Dear Lilian, You might want to try Biointerpreter (http://genotypic.co.in/bio_interpreter.html). It can take gene symbols and provide 15 different biological...
Dear Lilian, You might want to try Biointerpreter (http://genotypic.co.in/bio_interpreter.html). It can take gene symbols and provide 15 different biological...
Hello David, We use Agilent's linear amplification labeling kit for yeast (Saccharomyces,Candida and others) RNA labeling and it works great. We have...
Dear David, I have not heard of any complaints on this kit. Please call me at your convenience regarding this matter. Sincerely, Kevin White Technical Support...
Hi there, I have a question on Genotyping using real time PCR machine. I heard Applied Biosystem offer Taqman SNP genotyping assay which uses VIC/FAM...
Hi Lilian, The ontology analysis can be difficult and even with human genes you can get significant problems. DAVID used to take the GeneIDs as input as well,...
Hi Jean, There is a 'new to me' application called HRM (high resolution melting) available using an ABI 7500 fast instrument. Caprice S. Caprice Rosato...
Hi Jean, I have been used Taqman SNP genotyping (VIC/FAM label probes) assays in the Stragene Mx3005P machine . I have excellent results, I think that this...
Dear Jean: Commercial response...Microarrays can perform genotyping very well, have you considered that as an option? There are many good papers describing a ...
... \Invitrogen sells both Human Cot-1 and Mouse Cot-1. I'm still trying to find a Bovine Cot-1 supplier or Macaque George...
gmweissenberger
gmweissenberger@...
Jul 15, 2009 7:52 am
19780
Hi Jean, Monte replied earlier to your post and he is absolutely right: This easiest solution is to to simply pick the assay from the AB website. What species...
edward.enright
edward.enright@...
Jul 17, 2009 4:46 pm
19781
Is any one interested in genotyping using custom arrays? Raja ======================================= Dr Raja Mugasimangalam CEO, Genotypic Technology [P]...
Hello All, I would like to know the methods good enough to get an overview of the data obtained from microarray study. The details- After statistical analyses,...
Noel Mathur
noelmathur@...
Jul 21, 2009 2:42 pm
19783
Hello, We have been having issues with the elution of of our samples from the beads following PCR. When we add elution buffer, the bead suspensions do not look...
Hi Noel, BLAST2GO will perform robust GO analyses and also generate pie charts for you (if you export the data). It also generates KEGG pathways and runs ...
Paul Lang
paul.lang12@...
Jul 23, 2009 7:37 am
19787
Noel, Sorry to hear you have had trouble running Onto-Express. We will be very happy to help you get it to work on your computer. And, yes, it does create a...
Sorin Draghici
sorin@...
Jul 23, 2009 7:37 am
19788
A postdoctoral position in microbial plant pathogen genomics and bioinformatics is available in the laboratory of C. R. Buell in the Department of Plant...