Hello all,

I'm using Agilent arrays for gene expression studies and I have noticed that
some array images are obvious outliers as they are completely yellow and very
bright.  Has anyone seen this before or have any ideas as to what this could be
due to?

Brian




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qPCR 2010 Event  -  Call for Abstracts

www.qPCR2010-Vienna.net

-----------------------------------------------------

BioEPS GmbH is organizing the qPCR 2010 Event taking place April 7th – 9th, 2010
in Vienna, Austria. Scientists from all around the world will come to exchange
ideas, share experiences, and discuss the exciting future of the perhaps most
powerful analytical technology ever developed in the life sciences area – the
quantitative real-time polymerase chain reaction (qPCR).

We have the pleasure to announce the Call for Abstracts for the qPCR 2010 Event
!

Finally we want to present 40 scientific talks from international scientists and
diagnostic companies in the qPCR field who will show their latest research
findings and newest technologies. The focus of the qPCR 2010 Event will be "The
ongoing evolution of qPCR" representing all new and emerging techniques,
applications and data analysis methods.

Deadline is 31st January 2010   =>   http://submission.qPCR2010-Vienna.net


Talk topics:
--------------

MIQE and QM strategies in qPCR
The MIQE guidelines: minimum information for publication of quantitative
real-time PCR experiments. Following these guidelines will encourage better
experimental practice, allowing more reliable and unequivocal interpretation of
qPCR results. QM strategies in real-time PCR to guarantee better and more valid
results.
Keynote speaker:   Prof. Stephen Bustin

High throughput quantitative PCR – digital PCR
384 well applications, new high throughput platforms, droplet PCR, qPCR
robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA and
microRNA), quantitative multiplexing, …
Keynote speaker:   Prof. Mikael Kubista,   Dr. Philip Day,   Dr. Ken Livak

HRM – High Resolution Melting
SNP analysis, HRM = high resolution melt applications, Epigenetics, methylation
markers, HRM platform comparison, etc …
Keynote Speaker   Prof. Carl Wittwer,   Prof. Claudio Orlando

Circulating nucleic acids
Analysis of circulating RNAs and DNA and microRNAs as diagnostic and prognostic
marker, …
Speaker:   Dr. Pamela Pinzani,   Dr. Alfred Schöller,  Dr. Jim Huggett

Single–cell qPCR
single-cell sampling, pre-amplification techniques, laser micro-dissection,
sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection,
FACS spotting, single cell handling, pre-amplification, …
Keynote speaker:   Dr. Michael W. Pfaffl,   Dr. Anders Stahlberg

RNAi – microRNA – siRNA Applications – miRNA normalisation
RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect microRNA,
microRNA normalisation strategies, siRNA applications in combination with
qRT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and
microRNA technologies,
Keynote speaker:   Prof. Jo Vandesompele,   Dr. Mirco Castoldi

qPCR BioStatistics & BioInformatics
software applications, data mining, calculation of relative expression, primer
and probe design on mRNA and microRNA level, real-time PCR efficiency
determination, mathematical modelling, multivariate expression profiling,
statistics in real-time PCR, data management, multiway expression profiling,
multiple regression analysis, 3D data visualization, ...
Speaker:   Dr. Ales Tichopad,   Dr. Jan Hellemans,   Dr. Anders Bergkvist


An online registration and abstract submission software CONFTOOL is available
here  => http://registration.qPCR2010-Vienna.net


-----------------------------

About qPCR:
Using qPCR the amount of target nucleic acid in a complex sample can be
determined with high precision, great accuracy, excellent specificity and the
ultimate sensitivity of detecting a single molecule. The technique has
revolutionized all molecular sciences and diagnostic applications. Conference
presentations will include MIQE guidelines & QM strategies in qPCR, high
performance nucleic acid extraction, single-cell applications, Epigenetics &
High-Resolution-Melt analysis, circulating nucleic acids, and application
involving RNAi and microRNA.
Further developments of qPCR technology will be presented include improved
instrumentation, miniaturization, high throughput platforms, cost efficacy,
validity, flexibility, quality assessment and reliable Cq calculations,
expression data comparisons, and interpretation.
Today there is no field in the life sciences research, molecular biology and
diagnostics areas that has not introduced qPCR technology for nucleic acid
analysis. The combination with reverse transcription enables determination of
mRNA, microRNA and widely opens the window for "Transcriptomics" – the first
step of quantitative "Gene Expression Profiling" and "Functional Genomics".
An Industrial Exhibition will take place parallel to the symposium, with 32
leading biotechnology companies presenting their latest developments, including
real-time PCR cyclers, NA extraction robots, consumables, new fluorescence dyes,
NA detection and amplification chemistries, as well as real-time PCR data
analysis software.

For more information about the qPCR 2010 event contact Dr. Martina Reiter  
Martina.Reiter@...


Hope to meet you in April in Vienna!

Michael Pfaffl

Dear all,

I am studying a set of microarray data for human haematopoietic stem cells. I
want to relate the expression level to protein concentrations. For example, the
gene expression level PM/MM for a signaling protein wnt is 33.4. Is it possible
to relate this number to the concentration of wnt protein in the sample? Does
anybody know any related references? Any suggestions and helps will be
appreciated.

Thank you very much.
Wendy

Hi, Mary

We use those, too.  The part number I have is X7022, and this is for
the Genetix 384 well plates with lids.

Hope this helps,
Caprice

S. Caprice Rosato    <rosatoc@...>
Center for Genome Research and Biocomputing
Oregon State University
3012 Agriculture and Life Sciences
Corvallis, OR 97331
(541) 737-3413  FAX:(541) 737-3045
<http://www.cgrb.oregonstate.edu



On Nov 10, 2009, at 6:04 PM, mary_galli wrote:

> Hello--I am trying to find a supplier or source of the old model
> Genetix 384-well microplates that fit the Molecular Dynamics GenIII
> spotter. I believe the plate model# is X5005. Full Moon Bio no
> longer supplies these and as far as I know they are the only type of
> plate that fits in this particular arrayer. Any info or suggestions
> would be helpful. Thanks! Mary
>
>
>



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Email: sales@...

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[Non-text portions of this message have been removed]

Hello--I am trying to find a supplier or source of the old model Genetix
384-well microplates that fit the Molecular Dynamics GenIII spotter. I believe
the plate model# is X5005. Full Moon Bio no longer supplies these and as far as
I know they are the only type of plate that fits in this particular arrayer. Any
info or suggestions would be helpful. Thanks! Mary

Does anyone have or know where I can source, on-line or otherwise a user manual
or instruction guide for a GeneJet microarrayer produced in the late 1990's by
Combion?