microarray : Messages : 1-37 of 19897

An Open Letter to Members of the Gene-Arrays Listserv:

Hello Everyone,

A few issues regarding the Gene Arrays Listserv.

A: I would like to add to the announcement regarding the launch of the upcoming archive for the Gene Arrays Listserv as a result of the persistent efforts of Dr. Ruth Alscher of Virginia Tech. and the Ron Sederoff group in Forest Biotechnology at NC State Univ. Having spoken with Ruth in some detail here are some of the notable details of what will be available as a result of the new Web site;

1. The contents of the Gene-Arrays Listserv will be archived at the Virginia Tech. Web site.

2. The contents of the Gene-Array Listserv will be categorized in a manner to lend some organization to the otherwise unwieldy string of posts. I would like to encourage members to submit ideas for categories for the Web site which would assist users in searching the archive in an efficient manner.

3. Some editing will be performed to remove obviously unneeded posts i.e. sub./unsub. messages, sales pitches, and blatantly off-subject post (SPAM).

4. Lastly the archive will include a section allowing viewing of documents sent to the list as attachments, thereby precluding the need for downloading of attachments should subscribers wish to defer doing so for numerous reasons i.e. bandwidth limitations.

In light of the later, I think we can lay to rest the matter of the "attachment issue". However, it seems reasonable that each list member take it upon themselves to adjust their e-mail according to their needs, as has been suggested by many.

Although the new Web site will essentially render the "attachment" issue moot, a few other points regarding the posting of attachments, the credit for which in many cases lies with other list members, remain relevant and worth keeping in mind nevertheless.

1. The small "attachments" should be pasted in the text of the message and not sent as attachments; the choice being one of discretion and judgement (shall we say a 1 or 1 and half pages?).

2. Where attachments are appropriate, the size and format of the attachment should be clearly noted in the text of the list message (PDF is highly recommended for format retention). If the information is available on the Web, a URL should be clearly noted.

3. Every reasonable effort should be made to offer any and all attachments in the most universal format possible i.e. again PDF recommended as it is as close to "universal" that I know of).

4. When responding to the G.A. list where the original message has an attachment, please do not use the reply button, rather begin with a new message free of the attachment to insure that the attachment is not repeatedly being posted to no use whatsoever, but rather to the annoyance of everyone.

B: Miscellaneous Matters:

1. I would like to ask that those who have posted queries to the list and received responses privately please forward a "digest" or summary of the responses to the G.A. list at large for the benefit of those who share an interest in the response(s). Where for whatever reason the situation precludes full disclosure of information, we would ask that one simply provide what one can. Without going into detail, there is one case where an ongoing discussion apparently went "off-list" leaving a number of interested individuals in the cold, I happy to report that someone took it upon themselves to post a summary of the response for the benefit of everyone interested.

2. It should perhaps be point out that lest we loose the worthy contributions made by the many individuals who take the time and thought to offer help in answering the many questions that arise on the list, a measure of appreciation should be acknowledged when criticism is offered. I've noticed that often the efforts put forth by list members are on occasion met solely with criticism with little to suggest any appreciation of their efforts. It seems to me that while their efforts may not be to each of our individual sense of "perfection", the mere fact that they tried goes much further than ideal "ideation".

Lastly on another more uplifting note, I would like to take this moment of your time to thank the many people who have so often provided help in answering questions concerning the listserv and its workings. Often times a list member(s) has answered the query before I had even the chance to read it. On behalf of everyone on the G.A. list I would also like to acknowledge the many fine suggestions that have been put forth over the course of the year to the betterment of the list. For those who may recall, earlier this year the issue arose concerning moving the G.A. Listserv to form a Usenet grp.; I posted an open letter soliciting comments and opinions on the matter, the result of which was 6 unique responses split evenly. Please keep in mind this is everyone's list not mine, hence where and in what form we keep it alive is a collective issue.

Enough from me, I know this has run long. Thank you for your time and attention, and a safe and joyous Holiday Season one and all.

Chandi

Chandi Griffin
GCRC: Molecular Biology Core Laboratory & GCRC GeneChip Contact
Univ. of California, San Francisco/San Francisco General Hospital
1001 Potrero Ave. Bldg. 100, Rm. 285
Phone: (415) 206-8757
E-mail: aniya@itsa.ucsf.edu

#35

From: Ruth Alscher <ralscher@...>
Date: Tue Dec 21, 1999 1:13 am
Subject: website

ralscher@...

The url for the Virginia Tech/Forest Biotechnology, North Carolina State
University website is http://www.bsi.vt.edu/ralscher/gridit.  Parts of it
still need editing, especially categories for the archive, which will be
curated by me.  There are about 900 of you out there.  Please send
comments, complaints and even compliments to ralscher@....

Ruth

#34

From: Ruth Alscher <ralscher@...>
Date: Mon Dec 20, 1999 7:22 pm
Subject: Website

ralscher@...

The url for the Virginia Tech/Forest Biotechnology, North Carolina State
University website is http://www.bsi.vt.edu/ralscher/gridit/.  There are
spots that are still under construction, mostly concerned with categories
for the archive, which will be curated by me.  Comments, complaints, or
even compliments, are welcome.

There are some 900 of you.  I'm hoping for a bunch of responses!

Ruth

Reply | Forward | Messages in this Topic (8)

#31

From: Philippe MARC <pmarc@...>
Date: Sun Dec 12, 1999 4:41 pm
Subject: Re: Glass Slides

pmarc@...

Hello,

I heard about CEL slides:
http://www.cel-1.com/bio-molecular_products.htm
Prices are very low, but we didn't test it yet.

Regards,
Philippe

--
Philippe MARC
Laboratoire de Genetique Moleculaire
Ecole Normale Superieure -- 46, rue d'Ulm -- 75230 PARIS cedex 05
tel: 01 44 32 39 40
http://www.biologie.ens.fr/lgm.html
-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.
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javillonarc@... a �crit :

>
> Does anyone have a recommendation as far as which slides have the best
   results; that is lower background and higher affinity?  So far we have
   only tried the Corning GAP slides and we are looking at trying the
   Surmodics 3D slides and Telechem's super amine and super aldehyde
   slides.

> -----------------------------------------------------------------------
>                             [Click here!]

> eGroups.com Home: http://www.egroups.com/group/microarray
> www.egroups.com - Simplifying group communications

#30

From: javillonarc@...
Date: Fri Dec 10, 1999 8:55 pm
Subject: Glass Slides

javillonarc@...

Does anyone have a recommendation as far as which slides have the best
results; that is lower background and higher affinity?  So far we have
only tried the Corning GAP slides and we are looking at trying the
Surmodics 3D slides and Telechem's super amine and super aldehyde
slides.

#29

From: goossens@...
Date: Fri Nov 12, 1999 2:23 pm
Subject: Postdoctoral position

goossens@...

Our laboratory has two positions immediately available (one year with a
high probability of extension for one or two years): a postdoctoral
position and a laboratory technician position to work at INSERM unit 468
(University of Paris XII medical school, Hopital Henri Mondor, Cr�teil,
12 km from Paris) to study human molecular genetics. The scientific
projects include implementation and utilization of cDNA microarrays in
differential gene expression analysis in cellular and mouse models of
cystic fibrosis or neurocristopathies, and development of novel
high-throughput moleculardiagnostic systems. The postdoctoral candidate
should have a strong background in molecular biology. Experience and/or
a strong interest in bioinformatics would be an advantage.Contact: send
CV and names of three references to: Prof M. Goossens, Inserm U468,
Hopital Henri Mondor, 94010 Cr�teil, France. Fax (33) 149812219. Email:
goossens@....

#28

From: cspivey@...
Date: Thu Nov 11, 1999 8:30 pm
Subject: upcoming meetings

cspivey@...

WHAT'S WILD ABOUT MICROARRAYS


"It is a very sad thing that nowadays there is so little useless
information"
A wild idea, (Oscar Wilde 1854-1900) particularly for a biotech sector
struggling to digest an armada of information flowing from various
sequencing programs. What is required today is a reexamination of
system robustness and results reproducibility. It is also necessary to
consider where engineering developments are leading research, and what
consequences this will yield for drug development. The fantastic
advantages of miniaturization are obvious, and peewee petri's multiply
as their costs become manageable; but the leap to cash in on the
secrets of the sequence may leave some stranded on the wrong side of an
information canyon.


Call for posters,
CAMBRIDGE HEALTECH INSTITUTE'S
LAB CHIPS and MICROARRAYS EUROPE
JANUARY 17-19th, 2000 ZURICH, SWITZERLAND
DEC 10th deadline, see http://www.healthtech.com for more details


First Announcement and Call for Papers;
CAMBRIDGE HEALTECH INSTITUTE'S
LAB CHIPS and MICROARRAYS - JAPAN
MAY 8-9TH 2000, TOKYO, JAPAN

Topics to be covered are:  microfluidics and microfabrication,
lab-on-a-chip, microarray results, clinical applications, genomic
studies,
POC diagnostics.


First Announcement and Call for Papers;
CAMBRIDGE HEALTECH INSTITUTE'S
MACRO RESULTS from MICROARRAYS: Applications for Drug Development
Tentative dates APRIL 17-19TH 2000, PHILADELPHIA, USA (check WEB site
www.healthtech.com for confirmation)

Topics to be covered are: validation and standardization, production,
detection, alternative techniques, protein chips, consumables
advances,library interface and combinatorial synthesis, utility,
integration,TADD (transcript-aided drug design) and intellectual
property.

Those researchers working in the technical developments of this field
and
those researchers utilizing these new applications are encouraged to
submit
a proposed title and 3-4 sentence pr�cis, detailing their work for
consideration of presentation by the scientific advisory committee.

Please indicate for which meeting your proposal applies.
Submission deadline, Friday November 19th, 1999.



Chris Spivey
Conference Producer
Cambridge Healthtech Institute
ph    617-630-1373
fax   617-630-1325
Email cspivey@...

#27

From: stephen.ayer@...
Date: Tue Nov 2, 1999 9:18 pm
Subject: PCR products vs. cDNA clones

stephen.ayer@...

Hi,

I'm new to the microarray field and I was wondering what others opinion
was regarding arraying cDNA clones (plasmid DNA) vs. arraying PCR
products made from the cDNA clones.

Is there a high level of background from plasmid DNA due to vector
sequence? Or could plasmids be used to do a differential expression
type experiment?

Thanks
Stephen Ayer

#26

From: crtomlinson@...
Date: Thu Oct 28, 1999 4:27 pm
Subject: thermocyclers

crtomlinson@...

Dear Members,



We are setting up a genomics lab at Univ Cincinnati Med Ctr for DNA
chip and

microarray technology, and we are now ordering the equipment.  Please

recommend a high volume, dependable, robotic-compatible PCR thermocyler.

Your input would be most appreciated.



Sincerely,





Craig R. Tomlinson, Ph.D.

1012 Clifton Hills Ave.

Cincinnati, OH  45220

T: (513) 569-9414

F: (513) 569-9414*51

e-mail:  crtomlinson@...

#25

From: pmarc@...
Date: Wed Oct 20, 1999 2:50 pm
Subject: Re: scanners

pmarc@...

We have a Scanarray 3000 (General scanning), it is a reliable scanner
(10 micron resolution, 10 minutes to scan a 6000 genes microarray). But
GS don't sell it anymore.
The Scanarray 4000 seems great to with a resolution of 5 micron, it
comes with a new software instead of Imagene (I think ScanAlyse still
the more efficient software). They have change the autofocus mode and
you can't change laser only or PMT only anymore.  ScanArray 5000 is the
same with multi lasers and autoloader.

We are looking for advise on axon and genetic microsystems scanners.
We tried this last one and we didn't find any difference with
ScanArray3000 except that the laser is stronger (not really good for Cy
Dies!)

Philippe

--
Philippe MARC
Laboratoire de Genetique Moleculaire
Ecole Normale Superieure -- 46, rue d'Ulm -- 75230 PARIS cedex 05
tel: 01 44 32 39 40
http://www.biologie.ens.fr/lgm.html
-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.   .-. .-.
||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\
|/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X
'   `-' `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'   `-' `-'   `-'

sde-@... wrote:
original article:http://www.egroups.com/group/microarray/?start=24
> Hi,
> We're starting a microarray program and currently looking into how
> we're going
> to set it up.  We have access to a GMS 417 Arrayer and will be using
a
> fluorescent microscope to do some initial reading, however we're
> looking into
> getting a scanner, so reading larger arrays will be easier and we'll
> have a
> data management set-up.
> Does anyone have recommendations for scanners? We'd like a set-up that
> has
> flexibility in the excitation and emission wavelengths, so we can use
> other
> fluorophores in addition to Cy3 and Cy5.
>
>
>
> Thanks,
>
> Sandhya Deo
> Godwin Group
> Department of Chemistry
> Northwestern University
>

Reply | Forward | Messages in this Topic (4)

#24

From: sdeo@...
Date: Thu Oct 14, 1999 4:33 pm
Subject: scanners

sdeo@...

Hi,
We're starting a microarray program and currently looking into how
we're going
to set it up.  We have access to a GMS 417 Arrayer and will be using a
fluorescent microscope to do some initial reading, however we're
looking into
getting a scanner, so reading larger arrays will be easier and we'll
have a
data management set-up.
Does anyone have recommendations for scanners? We'd like a set-up that
has
flexibility in the excitation and emission wavelengths, so we can use
other
fluorophores in addition to Cy3 and Cy5.



Thanks,

Sandhya Deo
Godwin Group
Department of Chemistry
Northwestern University

Reply | Forward | Messages in this Topic (4)

#23

From: astrid.lagreid@...
Date: Tue Oct 12, 1999 9:20 am
Subject: post.doc. position in NORWAY

astrid.lagreid@...

Post-doctoral position for molecular biologist at Functional Genomics project:

"Genomic Classifier Research - Mining genomic and clinical information for gastrointestinal and nevroendocrine-derived tumor classifiers"
http://www.idi.ntnu.no/grupper/KS-grp/compbiol/GCR.html

The project
"Genomic Classifier Research" is a multidisciplinary research effort involving scientists from the Faculty of Medicine, Faculty of Physics, Informatics and Mathematics, and Faculty of Humanities at the Norwegian University of Science and Technology (NTNU). The NTNU project is conducted in close collaboration with the Microarray Group at The Norwegian Radium Hospital, Oslo, Norway. The long term goals at NTNU are to gain new insight into molecular mechanisms for regulation of cellular, physiological and pathophysiological processes in the gastrointestinal system and in neuroendocrine-derived tumours, and to develop new methods and computer-based tools for efficient data analysis and medical diagnosis.
Our approach involves DNA microarray technology and specially designed software tools. DNA microarray printing and reading equipment is being built by the project group according to prototypes developed by Pat Brown, Stanford, and in Paul Meltzers group at NHGRI. The printer is already in use at the project. The reader (laser scanner) will be completed in October this year. The collection of cDNA probes available within the project comprises several thousand human and rat clones. Computer science methods for datamining and knowledge discovery have been developed by members of the group and are being implemented for biomedical problems within the research group.

The candidate
The project seeks a skilled scientist (PhD or equivalent qualifications) with substantial research experience in Molecular Biology and/or Molecular Physiology. Experience from Functional Genomics and/or Bioinformatics is an advantage.
The successful post.doc. candidate will work in a group with 20 participants from 5 different nationalities, including experts in medicine, molecular biology, computer science and ethics, where she/he will play an active role in the establishment, optimalisation and implementation of DNA microarray technology, and will be invited to work within one or several of the following biomedical problems: 1) Gastrin-mediated regulation of gene expression, 2) Neuroendocrine oncology; 3) Cancer of the Stomach and Oesophagus, 4) Somatic mutations in ECLomas. For references: Cancer 1998, 83: 435-444; Br J Pharmacol 1998, 124, 69-74; Path.Res.Pract. 194: 11-23, 1998; http://www.idi.ntnu.no/~janko/

Application and contact information
The position is open immediately, runs for three years, and is financed by The Norwegian Cancer Association. For additional information, please contact: Associate professor PhD Astrid L�greid (project leader), Department of Physiology and Biomedical Engineering, phone +47 73 59 86 16, fax +47 73 59 89 86, e-mail: astrid.lagreid@.... Contact in Bioinformatics: Professor PhD Jan Komorowski, Dept. of Computer and Information Science , phone +47 73 59 45 67, e-mail: jan.komorowski@....
The application must contain CV, list of publications and names of 3 references, and be sent to Astrid L�greid, Department of Physiology and Biomedical Engineering, Medisinsk teknisk senter, N-7489 Trondheim, NORWAY.

Application deadline November 1st 1999.

#22

From: <darryl_garrison@...>
Date: Fri Oct 8, 1999 7:04 pm
Subject: P33

darryl_garrison@...

I would like to inquire about using P33 labeled probes to hybridize to chips.
What instrumentation is being utilized to support this technique?  What kind of
success rates are people getting with this process?  Any supporting statistics
would be appreciated.

Darryl

#21

From: <darryl_garrison@...>
Date: Fri Oct 8, 1999 5:26 pm
Subject: Job opening

darryl_garrison@...

Interested candidates send resume/curriculum vitae, indicating Job Req. #,
to:STRATAGENE, Attn: Darryl Garrison, 11011 N. Torrey Pines Rd., La Jolla, CA
92037 or e-mail to darryl_garrison@... . Drug and alcohol testing
required. AA/EOE


Production Associate I
Responsible for process improvements, slide coating, DNA linkage, probe
preparation, chip hybridization, chip scanning, data analysis and QC. Requires a
BS/BA degree in Biological Science or related field and 2-3 years experience in
chemistry, molecular biology and/or biochemistry.  Computer literacy required.
Proficiency in laboratory techniques for molecular biology, including
non-radioactive labeling of nucleic acids, high throughput DNA analysis and PCR
cleanup, is needed.  The ability to perform experiments with an understanding of
the scientific method, including optimal experimental design for product
improvement, is necessary.   Manufacturing experience is essential, as is GMP
and documentation knowledge.  Ability to write and implement SOPs is essential.
Familiarity with robotic automation systems and/or micro-array/chip production
is a plus.  Job Req.  #32015-99A


Senior Production Assistant
Responsible for the preparation of chemical solutions, slide coating, slide
blocking, QC gels, running arrays, visual inspection of chips, and slide
packaging.  Requires a BS/BA degree in Biological Science or related field with
laboratory coursework in chemistry, molecular biology and/or biochemistry.
Knowledge of microbiology, or molecular biology is a must. Ability to prepare
chemical solutions, autoclaves items, aliquot solutions, run agarose gels,
maintain equipment, troubleshoot manufacturing problems is essential.  Computer
literacy and scientific notation knowledge are preferred.  Job Req. #31014-99A

#20

From: kdaustin@...
Date: Thu Oct 7, 1999 9:42 pm
Subject: Affymetrix-- any problems with chip supplies?

kdaustin@...

Affymetrix has grown so much lately, I'm wondering if there are likely
to be any problems regarding supplies of GeneChips.  Has anyone heard
of any difficulties or been told there will be a long wait after
ordering?

Thanks,
Katherine Austin, PhD

#19

From: rsmatson@...
Date: Thu Oct 7, 1999 5:43 pm
Subject: Focus Dinner at Chips to Hits'99

rsmatson@...

I will be moderating a focus dinner for microarray users during the IBC
Chips to Hits conference (Nov. 2nd-5th,Claremont Resort,  Berkeley,
CA). The  dinner hosts will be Beckman Coulter and Genzyme Molecular
Oncology .   The intent of the discussion following the dinner is to
survey microarray users and in particular  those users who have a need
(present or future) for automated handling to achieve higher
throughput.  If you are  interested  in attending such a dinner please
forward the following information: full name; company or
institution;professional title and position; telephone no., and email
address.

The following is a statement released  by the companies regarding their
intent:

" Beckman Coulter, Inc. is a leading provider of automated solutions
for life science research applications. We have strong competencies in
robotics, liquid handling and detection systems.  We see an opportunity
to integrate innovative array printers and readers with BCI robotics,
consumables, and software.  Our technical knowledge of microarrays, and
access to key array intellectual property, are the foundation for a
collaboration with Genzyme Molecular Oncology in gene expression arrays
for bioresearch. GMO has substantial gene IP assets.  We are in the
assessment phase of a partnership with GMO to offer a complete customer
solution."

#18

From: pmarc@...
Date: Sat Oct 2, 1999 9:34 am
Subject: forum GeneSpringTM

pmarc@...

Here is a more specialised group. Created by Tony Lialin (
tlialin@... ) from Silicon Genetics ( http://www.sigenetics.
com/ ).

>GeneSpringTM Users Group Announcement:
>A virtual newsgroups for GeneSpringTM Expression Analysis Software
users has been created at: http://www.egroups.com/
>To subscribe, send a message to genespringusers@egroups.com. or go to
http://www.egroups.com/group/genespringusers/

Approval is required to join the group
(info found in the GENE-ARRAYS list)

#17

From: m_megginson@...
Date: Fri Oct 1, 1999 1:26 pm
Subject: Re: Affy vs. Glass

m_megginson@...

We have placed a good share of the biochip scanners and it is rare that
individuals have asked about the Affy density patent. I think Affy does
not want to play big brother and stifle the market. Buying Genetic
Microsystems legitimizes the glass slide technology approach.

#16

From: pmarc@...
Date: Thu Sep 30, 1999 7:18 am
Subject: Post-doctoral position

pmarc@...

Sorry,I didn't find English version of this.

> ABGf-74 - Stage post-doctoral : Analyse d'images et Analyse de
donnees dans
> l'utilisation des puces a ADN.
>
> Date de debut de diffusion du poste : 24/09/1999
>
> L'Institut Pasteur met en place une plate-forme technologique pour la
> production et l'utilisation des puces a ADN dans l'etude des maladies
> infectieuses. Ces technologies nouvelles requierent de nombreux
developpements
> en bio-informatique et en analyse de donnees. Le site souhaite mettre
en place
> un outil coherent integrant l'analyse d'images, une structure de base
de
> donnees et les outils necessaires a l'exploitation de ces donnees.
>
> Le candidat sera integre au sein du laboratoire d'Analyse d'Images
> Quantitative et travaillera en interaction avec d'autres equipes de
l'Institut
> Pasteur impliquees dans la partie bio-informatique de ce projet, en
> particulier dans la realisation des bases de donnees (Laboratoire
Genomique
> des Microorganismes Pathogenes).
>
> Dans un premier temps, le stagiaire mettra en place un logiciel
d'analyse
> d'images pour la quantification automatique des signaux
d'hybridation. Ces
> valeurs, apres validation statistique, seront introduites dans une
base de
> donnees qui incluera des donnees d'origine multiple. La seconde
partie du
> stage concernera l'integration de methodes d'analyse de donnees dans
> l'exploitation des resultats.
>
> Le candidat devra avoir une solide experience en informatique et dans
l'un des
> domaines suivants : analyse d'images, analyse numerique et
statistiques.
>
>
>
> Contactez, de la part de l'Association Bernard Gregory, et en
precisant la
> reference de l'offre :
>
> Jean-Christophe Olivo-Marin (jcolivo@...) Laboratoire AIQ
> ou
> Philippe Glaser (pglaser@...) Laboratoire GMP
>
>
> Institut Pasteur
> Departement de Biologie Moleculaire
> 25 Rue du Dr Roux
> 75724 Paris cedex 15
> FRANCE

#15

From: <darryl_garrison@...>
Date: Tue Sep 28, 1999 10:28 pm
Subject: Job openings

darryl_garrison@...

Interested candidates send resume/curriculum vitae, indicating Job Req. #, to:
STRATAGENE, Attn: Professional Staffing, 11011 N. Torrey Pines Rd., La Jolla, CA
92037 or e-mail to Jobs@... . Drug and alcohol testing required.
AA/EOE.

Production Associate I
Responsible for process improvements, slide coating, DNA linkage, probe
preparation, chip hybridization, chip scanning, data analysis and QC. Requires a
BS/BA degree in Biological Science or related field and 2-3 years experience in
chemistry, molecular biology and/or biochemistry.  Computer literacy required.
Proficiency in laboratory techniques for molecular biology, including
non-radioactive labeling of nucleic acids, high throughput DNA analysis and PCR
cleanup, is needed.  The ability to perform experiments with an understanding of
the scientific method, including optimal experimental design for product
improvement, is necessary.   Manufacturing experience is essential, as is GMP
and documentation knowledge.  Ability to write and implement SOPs is essential.
   Familiarity with robotic automation systems and/or micro-array/chip
production is a plus.  Job Req.  #32015-99A


Senior Production Assistant
Responsible for the preparation of chemical solutions, slide coating, slide
blocking, QC gels, running arrays, visual inspection of chips, and slide
packaging.  Requires a BS/BA degree in Biological Science or related field with
laboratory coursework in chemistry, molecular biology and/or biochemistry.
Knowledge of microbiology, or molecular biology is a must. Ability to prepare
chemical solutions, autoclaves items, aliquot solutions, run agarose gels,
maintain equipment, troubleshoot manufacturing problems is essential.  Computer
literacy and scientific notation knowledge are preferred.  Job Req. #31014-99A





------------------------------------------------------------------------

eGroups.com home: http://www.egroups.com/group/microarray
http://www.egroups.com - Simplifying group communications

#14

From: mel_kronick@...
Date: Sun Sep 26, 1999 7:05 pm
Subject: microarray analysis conference

mel_kronick@...

A conference focusing on analysis and statistical issues regarding
microarrays is being organized through the auspices of the Lake Tahoe
Center.  The conference will be held November 9 to 12, 1999, at the
Granlibakken Hotel at North Lake Tahoe, California.  For details, see
the web site

http://www.laketahoesymposia.org/microarr.htm#Top

#13

From: rsmatson@...
Date: Sat Sep 25, 1999 1:30 am
Subject: Chips to Hits Conference

rsmatson@...

If anyone is going to the IBC Conference (Nov. 2-5,1999 @Berkeley,
CA)feel free  to introduce yourself.  I will be chairing Track A on
Biochips. We are also seeking individuals to participate in a private
dinner discussion regarding some new technology.

#12

From: rsmatson@...
Date: Wed Sep 22, 1999 7:36 pm
Subject: Affy vs. Incyte

rsmatson@...

Apparently my previous message was deleted.  Here is a re-take.  The
Affymetrix patent(s) cover spot density (>400 spots/sq. cm) as well as
2-color normalization for gene expression analysis regardless of how
the chips are fabricated.  These applications had early filing dates
than the Stanford patent of Brown and Shalon (Synteni/Incyte).  The
2-color claim was granted to Affymetrix over Stanford; and Incyte has
failed to convince the patent office of their position. The
infringement suit is still pending. However, currently with this
decision Affymetrix is free to pursue other business opportunities in
the area of spotted arrays in which Genetic Microsystems participates.
Incytes array program as well as others maybe in trouble.

#11

From: rsmatson@...
Date: Wed Sep 22, 1999 4:49 pm
Subject: Affy vs. Incyte

rsmatson@...

Affymetrix patents cover probe density (i.e. >400 spots/sq. cm)  and
2-color normalization for expression arrays regardless of the approach
to fabrication. Both patents mentioned were filed well in advance of
the Brown/Shalon (Synteni) Stanford patent and Incyte has apparently
failed to prove otherwise. The purchase of Genetic Microsystems
following the ruling allows Affymetrix to play in the spotted
(microarray) business.

#10

From: watsons@...
Date: Wed Sep 22, 1999 2:27 pm
Subject: Affymetrix vs Incyte Patent issue

watsons@...

Does anyone have a clear view of the key issues raised by the US patent
office ruling in Affy's favor and against Incyte last week? Is it
related to Affy's purchase of Genetic Mirosystems? I am wondering how
to see Incyte's position with respect to their arrays.

Stan Watson

From: manuel <mduval@...>
Date: Fri Sep 17, 1999 9:46 pm
Subject: Re: pin technology

mduval@...

We are currently using pins manufactured by Die-Tech and these are the best
we got so far.  We cleaned them before use by ultrasonication for
approximately one minute.
The URL of Die-Tech's web-site: http://pw2.netcom.com/~die-tech/.
If you purchase 32 pins, the price is around $70/pin.
Manuel






Manuel Duval, PhD
Assistant Research Scientist
Department of Biology
Terry Thomas's lab.
College-Station, TX 77843
phone (409) 845 0663
Fax  (409) 847 8805
<mduval@...>

From: rsmatson@...
Date: Fri Sep 17, 1999 6:19 pm
Subject: pin technology

rsmatson@...

There are a number of printing pins beginning to appear on the market
such as Telechem and Majer.  We have heard that certain pins require
the user to sculpture these for use. Does anyone have any experience
with the various kinds of pins that they could share on the subject?
Our own experience is that the pins are quite variable in delivery.

From: pmarc@...
Date: Thu Sep 16, 1999 1:42 pm
Subject: delivery: mail, summaries or digest

pmarc@...

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From: "Philippe MARC" <pmarc@...>
Date: Wed Sep 15, 1999 1:08 pm
Subject: Welcome to the microarray eGroup

pmarc@...

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