I feel we should not have any arbitrary standards. We need to be flexible in the solutions we consider. If there is a good reason to only remove 80% of the lipids or if it just less costly, let's go for it.
We do not necessarily need 100% separation of components. More to the point, we only need the froth flotation to remove about 25% of the algae cells from the culture. Your supposition may be the key to why people think froth flotation is considered expensive. If someone has to work it really hard to get all the algae out of the culture, the cost may be much higher than trying to get a fraction of the cells out.
I don't feel getting the lipids pure is essential. There are lots of processes to filter and clarify lipids. I assume they will be added to the end of our process, if needed.
I fear that Brian may be correct that we can not just dump the algae debris back into the culture and expect the still growing algae cells to be able to break it down and use the nutrients. That may especially be true with the lipids. We may have to compost, aerobically digest, or otherwise treat the cell debris and unextracted lipids before we can return them to the culture.
Even if that processing cost more than it is worth, we still may be satisfied with extracting on 80% of the lipids. For instance, if we sell the cell debris for animal feed, the farmers may want us to leave some of the lipids in the press cake - the animals may need a little fat in their feed.
(Reality note - accepting less than perfect processes is just to get started with. Once we have a process that allows us to make a profit, we will keep working on each part of it, trying to get closer to perfection.)
Bobby
Bobby:ÂI can see your point that removing algae first makes it easier to then separate lipids from the aqueous phase.  But what if we are satisfied with say 80% removal of lipids from a mixed media and then recycle that media back to the algae growing units? Am I smoking something illegal by suggesting this?ÂÂIntuitively I do prefer froth flotation for what you are suggesting, but at least one source claims it is too costly for now - http://en.phyco.org/wiki/Harvesting ? I do not see how unless the objective is to approach 100% separation of all components. Your comment?ÂConceptually the only important pure product from my perception is the lipids. I am not sure I'd mind if some percent of the lipids cannot be removed in sufficient pure form as long as they can be recycled. Why is that perception faulty?ÂCheersAlexÂ-------Original Message-------ÂÂAlex,
First we have to get the algae out of the culture.
Removing lipids is second.
I assume your point was that we should not use a treatment that prevents us from some other processing we would want to do. A good thought, but we might end up doing it if that is a drastically cheaper process.
It does give another reason for my favorite - Froth flotation.
Until we have run a lost more tests and costed out a lot of techniques, we need to keep our minds open to all possibilities.
Bobby
On Fri, Jul 3, 2009 at 10:13 AM, Alex Markin <anzactwo@...> wrote:
Hi Bobby:ÂLet's get back to the objective of algae processing. Aren't we trying to remove lipids/oils/organics from the mass and then recycle what is left? Isn't that the ideal? ÂÂIf so, then the next question is what has to be done to recycle or alternatively use the processed algae as fuel or further fermentation, the latter assuming somewhere down the road cellulosics can be cost-effectively fermented to alcohols.ÂCheersAlexÂ-------Original Message-------ÂÂAlex,
Reading between the lines, there may be some info here that could help when trying to floc out algae.
Also, it says that alum can give a boost to sand filters.
I still like the idea of froth flotation, but who knows what is going to be cost effective?
BobbyOn Thu, Jul 2, 2009 at 12:08 PM, Alex Markin <anzactwo@...> wrote:
See  http://WWW.southshoregunitepools.Com/resources/pdfs/how_alum_works.pdf Alum works to remove suspended particles, not emulsions. Does algae stay in suspension when processed? I'd guess filtration (various options depending on porosity of the resulting cake and gunking) would remove most of it. If not I do not see how flocculants would do anything for algae sourced emulsions.ÂI'd guess an emulsion breaker and demulsifiers separating phases is what you are looking for; not flocculants. An example - http://www.champ-tech.com/onec_prod_emul.aspÂCheersAlexÂPS: In my previous life I was a chemical engineer and have remained heavily involved in the chemical industry world-wide.Â-------Original Message-------ÂSubject: Re: [oil_from_algae] Re: alum???ÂBruce,
Do you think it might work as a flocculant?
I am also considering using it to remove gypsum from drinking water.
BobbyOn Wed, Jul 1, 2009 at 8:05 AM, lendlabs <lendlabs@...> wrote:
???Alum (pronounced /ˈæləm/) is both a specific chemical compound and a class of chemical compounds. The specific compound is the hydrated aluminum potassium sulfate with the formula KAl(SO4)2.12H2O. The wider class of compounds known as alums have the related stoichiometry, AB(SO4)2.12H2O.
--- In oil_from_algae@yahoogroups.com, Bobby Yates Emory <liberty1@...> wrote:
>
> Ron and Gianfranco,
>
> I think that is commonly called Alum and is easily available - this is
> WalMart's listing:
>
> <
> http://www.walmart.com/search/search-ng.do?search_constraint=0&ic=48_0&search_query=alum&Find.x=11&Find.y=8&Find=Find
> >
>
> Bobby
>
> 2009/7/1 Gian Roncolato alchemirg@...
>
> >
> >
> > Dear Ron,
> > In the water treatment industries to clarify the water and flocculatind all
> > te suspended solids it is normally used "Aluminum Sulfate" (liquid form
> > about 8% content), can be used fram 10 to 25 grams per cubic meters.
> > It is not expensive and it work perfectly. It need a "pre-dilution" with
> > water followed by a distribution and good mixture inside the tank.
> > Regards
> > Gianfranco Roncolato
> >
> > ------------------------------
> > To: oil_from_algae@yahoogroups.com
> > From: potrzebie44@...
> > Date: Tue, 30 Jun 2009 13:23:21 -0700
> > Subject: [oil_from_algae] Learning from Failure [1 Attachment]
> >
> > [Attachment(s)<http://co119w.col119.mail.live.com/mail/EditMessageLight.aspx?ReadMessageId=fa6d5360-f2a2-4af6-9a38-e81dcc479708&FolderID=00000000-0000-0000-0000-000000000001&CP=-1&n=1316965772&Action=Reply&AllowUnsafe=False#TopText>from Ron Brown included below]
> >
> > The attached photo shows an algae culture we grew from one slant of
> > Chlorella vulgaris that we got from Carolina Biological Supply Company
> > ($9.00 + $30.00 s&H) in one week. I count that part as a success. But we
> > tried to flocculate it with lime, and that was a little disappointing.
> > "Microalgae Harvesting and
> > Processing: A Literature Review" discusses the use of lime to flocculate
> > algae. a quick search of the internet confirmed that lime has been used to
> > control algae in ponds. Well, it was quick, cheap and easy, but it didn't
> > work. We applied a pound or two of lime that was left over from a
> > construction project to about 300 gallons of culture. I was hoping to be
> > able to scoop up the floc with a household strainer. Although there was a
> > fine white precipitate on the bottom, there was no sign of flocculation.
> >
> >
> > ---rsb
> > Ron Brown
> > 30JUNE2009
> >
> >
> >
> > ------------------------------
> > What can you do with the new Windows Live? Find out<http://www.microsoft.com/windows/windowslive/default.aspx>
> >
> >
>
>
>
> --
> Toward freedom,
>
> Bobby Yates Emory
>
--
Toward freedom,
Bobby Yates Emory
Â
--
Toward freedom,
Bobby Yates Emory
Â
--
Toward freedom,
Bobby Yates Emory
Â
--
Toward freedom,
Bobby Yates Emory