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Dear Jo
Would you please have a look to the questions bellow that rose during an internal meeting?
1) What is/are the advantage(s) to use the mean Ct for calculating the relative quantities (qBase) instead of the min Ct (GeNom)?
2) Knowing such different approach (qBase vs. GeNorm), why is it necessary to estimate firstly the ref. genes in GeNorm and then use them in qBase if we are not applying the same method for calculating the relative quantities.
3)
Would it be possible to implement the GeNorm approach in qBase allowing then comparisons?
Adrian
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