Damon,

Inter-run calibration needs to be performed for every single gene
since run-to-run variation can be gene (mastermix, ...) dependent.

--- In qBase@yahoogroups.com, "damon.tumes" <damon.tumes@...> wrote:
>
> Hi, I have one more question about the inter run calibration performed
> by qbase. If I decied to only include IRC's for one or two control
> genes in my analysis wil qbase use these genes to perform inter run
> calibration on all samples (i.e. all genes in an experiment that are
> present on different runs with just the control IRC's) or will inter
> run calibration only be performed on those genes that have IRC's
> present?
>
> Best Regards,
>
> Damon
>