hi everyone,

i m not jst a new user of qbase, and also new in the lab. i did my
quantification pcr on the bio-rad icycler and annlysed the data by
the qBase. now, i meet a problem---how can i do my statistical
analysis using the analysed data ? my experiment is to examine 7
kinds gene expression after treatment and compare the expression
level with the control group( untreated cells). I detected 7 kinds
gene expression level at 5 different timepoints after treatment and
did a duplicate for every sample. according to the qbase output, i
can see the expression level change but i would know further ---- is
this level change statistical significant or not ?? BTW, i also used
two house keeping genes to normalize the RNA amout, but NF outcome is
not ideal ranging from 0.05 to 30,and both of them having a M value
0.96. i searched some relative messages in our group, it seems in my
actual case, i have to add another housekeeping gene in to get a
around 1 NF. dose my saying make any sense? i used the same amount
total RNA doing the RT, and i checked the RNA quality by running a
gel indicating clear nice bands of 28s and 18s.

i m sorry if my Q sounds stupid but i just cant make it by my own.
any help would be appreciated!

thnx